Monoclonal Anti-B as a New Blood-Typing Reagent

Sacks, S.H.; Lennox, E.S.

doi:10.1159/000464178

Cited by 20 publications

(22 citation statements)

References 12 publications

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“…The mAbs YTH 53.1 (rat IgG2b), YTH 89.1 (rat IgG2b anti-red cell glycophorin A), YTH 66.9 (rat IgM ; CAMPATH-1), and YTH 3.2 (rat IgG2b CD7 ; CAMPATH-2) were obtained from the fusion of a DA rat spleen with the myeloma line Y3/Ag1 .2 .3 (21). The anti-blood group mAbs NBl/19.112.28 (mouse IgM anti-B) and A15/3D3.92 .1 (mouse anti-A) were the kind gift of Dr. E. Lennox, Celltech, Slough, U.K. (22,23). Polyclonal antiserum against MAC-inhibiting protein was the gift of M. J .…”

Section: Methodsmentioning

confidence: 99%

CD59, an LY-6-like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells.

Davies

Simmons

Hale

et al. 1989

The Journal of Experimental Medicine

584

333

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The lysis of cells by complement requires only the terminal components C5, C6, C7, C8 and C9 and is initiated by the cleavage of C5 to C5b. Sequential addition of C6, C7, C8, and C9 to C5b leads to the formation of the membrane attack complex (MAC)' which, when inserted into the lipid bilayer, can form transmembrane pores (1-5). It is well known that when complement of one species is activated on homologous erythrocytes, lysis is much less efficient than when it is activated on other species of cell, and even among different heterologous cell species the lytic efficiency may be very different. It has long been known that the basis of this variable lytic efficiency is found, at least in part, at the C8 and/or C9 step (6-9). More recently, specific membrane proteins have been described that appear to protect cells from homologous complement . The first of these to be described was the decayaccelerating factor (DAF), a membrane protein of -70 kD molecular mass (10). This protein interferes with the assembly of the C3 converting enzymes both of the classical and alternative pathway (10, 11) and therefore it has only indirect effects on the cell lytic mechanism. A further membrane protein that does restrict homologous lysis, and that has been described both as the C8-binding protein (C8bp) (7, 12) and as homologous restriction factor (HRF) (13), has also been isolated . It seems likely that both these descriptions apply to a single protein of 65 kD molecular mass. In addition, a 55/65-kD MAC-inhibiting protein (MIP) with the capacity to bind C8 and C9 has been identified both on human erythrocyte membranes and in normal human serum (14). The relationship ofthis to HRF/C8bp is not yet clear. Both DAF and HRF/C8bp are bound on cell membranes by a glycolipid anchor (15, 16) and can be eluted from the cell membrane, at least in part, by phosphatidylinositol-specific phospholipase C. These proteins also have the capacity when they are isolated from

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Section: Methodsmentioning

confidence: 99%

CD59, an LY-6-like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells.

Davies

Simmons

Hale

et al. 1989

The Journal of Experimental Medicine

584

333

View full text Add to dashboard Cite

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“…A number of laboratories have produced such reagents [1][2][3]. The use of serological reagents based on monoclonal antibodies for blood typing can offer signif icant advantages over the use of traditional polyclonal products in terms of specificity, cost, batch-to-batch consis tency, availability, laboratory safety and in the release of human plasma for the production of therapeutic products.…”

Section: Introductionmentioning

confidence: 99%

Stability of Murine Monoclonal Anti-A, Anti-B and Anti-A,B ABO Grouping Reagents and A Multi-Centre Evaluation of Their Performance in Routine Use

McGowan¹,

Tod²,

Chirnside³

et al. 1989

Vox Sanguinis

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We have previously reported the production of 3 murine monoclonal reagents for ABO typing (designated ES-9, ES-4 and ES-15). This study presents results of tests of stability of these 3 reagents, together with a fourth murine monoclonal antibody (LM103/107). In addition, data are also presented from a multi-centre evaluation of the performance of the murine monoclonal reagents in routine ABO typing of both donors and patients using a wide variety of techniques, both manual and automated. The potency and stability of the 4 monoclonal antibody based reagents is compared with a broad selection of monoclonal and polyclonal ABO typing reagents. The reagents used for comparison were produced by European and United Stated manufacturers in both the public and private sector and are widely used in routine ABO typing. The Scottish monoclonal reagents have been used successfully to ABO type over 500,000 blood samples in 7 centres within the UK, with no discrepant results.

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“…Good blood grouping reagents have already been produced, some of them being superior to conventional human alloantisera. Most of the MAbs produced by this technique were directed against antigens of the ABO system: anti-A, anti-B, anti-A, B and anti-H [2][3][4][5][6][7][8]. Several other MAbs have been produced against other systems: anti-N, anti-M, anti-Lea, anti-Leb [9][10][11][12][13][14][15], but very few MAbs were specific for the Rh system [10], The anti-Rh MAbs described to date have recognized public determinants such as Rh 17, Rh29orRh 32 [16].…”

Section: Introductionmentioning

confidence: 99%

Murine Monoclonal Antibody Suitable for Use as an Rh Reagent, Anti-e

Bourel¹,

Lecointre²,

Genetet³

et al. 1987

Vox Sanguinis

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The production by the hybridoma technique of a monoclonal antibody which behaves as an anti-e agglutinin is described. A reagent was made from ascitic fluid diluted in RPMI 1640 culture medium and bovine serum albumin. All red blood cells bearing the e antigen were agglutinated; no reactivity was recorded with untreated EE red cells suspensions. However, cross-reactivity was observed with enzyme-pretreated EE cells. The characteristics of this monoclonal antibody are described and are in accordance with the standard references.

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Monoclonal Anti-B as a New Blood-Typing Reagent

Cited by 20 publications

References 12 publications

CD59, an LY-6-like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells.

CD59, an LY-6-like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells.

Stability of Murine Monoclonal Anti-A, Anti-B and Anti-A,B ABO Grouping Reagents and A Multi-Centre Evaluation of Their Performance in Routine Use

Murine Monoclonal Antibody Suitable for Use as an Rh Reagent, Anti-e

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