The production by the hybridoma technique of a monoclonal antibody which behaves as an anti-e
agglutinin is described. A reagent was made from ascitic fluid diluted in RPMI 1640 culture medium and bovine
serum albumin. All red blood cells bearing the e antigen were agglutinated; no reactivity was recorded with untreated
EE red cells suspensions. However, cross-reactivity was observed with enzyme-pretreated EE cells. The characteristics
of this monoclonal antibody are described and are in accordance with the standard references.
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