A study of the mechanism of small-molecule soybean-protein-derived peptide supplement to promote sleep in a mouse model

Yi, Guofu; Safdar, Bushra; Zhang, Yihao; Li, You; Liu, Xinqi

doi:10.1039/d0ra00389a

Cited by 9 publications

(6 citation statements)

References 39 publications

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“…The overexpression of certain cytokines has been linked to the presence of certain clusters of QoL, including sleep disturbances (Neefjes et al, 2013). In this study, inflammation was significantly reduced in the peptide group probably in response to improved nutritional status, and we speculate that this may have impacted the differences in the trend of sleep disturbances between the two study arms, which is in agreement with findings by Yi et al (2020).…”

Section: Discussionsupporting

confidence: 88%

Soypeptide supplementation attenuates weight loss and improves quality of life among brain cancer patients in a pilot study

Amakye

Huang

et al. 2023

Food Frontiers

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Inability to meet nutrition needs resulting from multiple diseases‐related, and individual factors contribute significantly to malnutrition and poor disease outcome among cancer patients. Strategies capable of delivering metabolically efficient nutrients with less digestive and metabolic stress without adding bulk to the diet of patients may be suitable for dealing with cancer‐related malnutrition and quality of life (QoL). We sought to test the hypothesis that peptide supplementation could attenuate weight loss and improve QoL among brain cancer patients compared to conventionally treated controls. In a non‐blind pilot study, brain cancer patients received 9 g soybean peptide supplementation twice per day (peptide group, n = 9) and compared with conventional treatment (control group, n = 10) over two chemotherapy cycles of 42 days. Compared to the controls, soypeptide supplementation attenuated weight loss with 5.4% increase in weight. Moreover, QoL, assessed using the European Organization for Research and Treatment of Cancer QLQ‐C30, revealed that physical (8.1 ± 15.6, p < .047) and emotional (7.5 ± 3.5, p < .033) functioning as well as summary scores (7.1 ± 2.4, p < .048) improved, whereas others remain stable. Molecular docking simulation suggested that the peptides could induce body weight through the inhibition of angiotensin‐converting enzyme (ACE) upon digestion. Daily soypeptide supplementation could enhance weight gain and improve QoL among brain cancer patients possibly by inhibiting ACE activity. However, a follow‐up study with a larger sample size in a double‐blind randomized trial is required to validate these preliminary findings.

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Section: Discussionsupporting

confidence: 88%

Soypeptide supplementation attenuates weight loss and improves quality of life among brain cancer patients in a pilot study

Amakye

Huang

et al. 2023

Food Frontiers

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“…SBPs were prepared according to research reports in our laboratory. In short, soybean protein is mainly obtained by enzymatic hydrolysis of various proteases [ 14 ]. As shown in Figure 5 , the single peptide of 99% purity was synthesized and bought from GL Biochem Ltd., (Shanghai, China), TSP.…”

Section: Methodsmentioning

confidence: 99%

“…Foods rich in tryptophan not only promote sleep but also prevent neurodegenerative diseases [ 12 ]. Soybean protein is generally known as full-value protein, rich in tryptophan as well as soybean peptides (SBP) prepared by enzymatic hydrolysis that can resist oxidative stress, promote sleep, be free of allergies, and can be widely consumed [ 13 , 14 , 15 , 16 , 17 , 18 ]. Moreover, compared to drug prevention, SBPs are safe and have no side effects as a food source.…”

Section: Introductionmentioning

confidence: 99%

“…However, there is a lack of information on the influence of SBPs on oxidative damage to nerve cells and their neuroprotective mechanism, especially about SBPs involvement in SIRT3-mediated neuroprotection. According to our previous study, the single peptides (SWGEDWGEIW, TSP) with higher content and containing tryptophan were identified by Nano-LC/MS-MS from SBP [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

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The SWGEDWGEIW from Soybean Peptides Reduce Oxidative Damage-Mediated Apoptosis in PC-12 Cells by Activating SIRT3/FOXO3a Signaling Pathway

Zhou

et al. 2022

Molecules

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The goal of the investigation was to study the protective effects of the SWGEDWGEIW (the single peptide, TSP) from soybean peptides (SBP) on hydrogen peroxide (H2O2)-induced apoptosis together with mitochondrial dysfunction in PC-12 cells and their possible implications to protection mechanism. Meanwhile, the SBP was used as a control experiment. The results suggested that SBP and TSP significantly (p < 0.05) inhibited cellular oxidative damage and ROS-mediated apoptosis. In addition, SBP and TSP also enhanced multiple mitochondrial biological activities, decreased mitochondrial ROS levels, amplified mitochondrial respiration, increased cellular maximal respiration, spare respiration capacity, and ATP production. In addition, SBP and TSP significantly (p < 0.05) raised the SIRT3 protein expression and the downstream functional gene FOXO3a. In the above activity tests, the activity of TSP was slightly higher than that of SBP. Taken together, our findings suggested that SBP and TSP can be used as promising nutrients for oxidative damage reduction in neurons, and TSP is more effective than SBP. Therefore, TSP has the potential to replace SBP and reduce neuronal oxidative damage.

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“…Another way SBPs inhibiting on apoptosis is via the activation of PI3K-AKT and inhibition of the apoptosis pathway [ 38 ]. SBPs regulate tryptophan hydroxylase (THP) and serotonin-N-acetyltransferase (AANAT) proteins to increase sleep [ 39 ]. SWGEDWGEIW (the single peptide, TSP) in SBPs reduces oxidative damage-mediated apoptosis in PC-12 cells by activating the SIRT3/FOXO3a signaling pathway [ 40 ].…”

Section: Introductionmentioning

confidence: 99%

The SWGEDWGEIW from Soybean Peptides Reduces Insulin Resistance in 3T3-L1 Adipocytes by Activating p-Akt/GLUT4 Signaling Pathway

Sang

Zhu

et al. 2023

Molecules

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Diabetes mellitus, a group of metabolic disorders characterized by persistent hyperglycemia, affects millions of people worldwide and is on the rise. Dietary proteins, from a wide range of food sources, are rich in bioactive peptides with anti-diabetic properties. Notably, the protective mechanism of the single peptide SWGEDWGEIW (TSP) from soybean peptides (SBPs) on insulin resistance of adipocytes in an inflammatory state was investigated by detecting the lipolysis and glucose absorption and utilization of adipocytes. The results showed that different concentrations of TSP (5, 10, 20 µg/mL) intervention can reduce 3T3-L1 adipocytes’ insulin resistance induced by inflammatory factors in a dose-dependent manner and increase glucose utilization by 34.2 ± 4.6%, 74.5 ± 5.2%, and 86.7 ± 6.1%, respectively. Thus, TSP can significantly alleviate the lipolysis of adipocytes caused by inflammatory factors. Further mechanism analysis found that inflammatory factors significantly reduced the phosphorylation (p-Akt) of Akt, two critical proteins of glucose metabolism in adipocytes, and the expression of GLUT4 protein downstream, resulting in impaired glucose utilization, while TSP intervention significantly increased the expression of these two proteins. After pretreatment of adipocytes with PI3K inhibitor (LY294002), TSP failed to reduce the inhibition of p-Akt and GLUT4 expression in adipocytes. Meanwhile, the corresponding significant decrease in glucose absorption and the increase in the fat decomposition of adipocytes indicated that TSP reduced 3T3-L1 adipocytes’ insulin resistance by specifically activating the p-Akt/GLUT4 signal pathway. Therefore, TSP has the potential to prevent obesity-induced adipose inflammation and insulin resistance.

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A study of the mechanism of small-molecule soybean-protein-derived peptide supplement to promote sleep in a mouse model

Abstract: Here, the effects of dietary supplementation with small-molecule soybean-protein-derived peptide (SBP) on sleep duration in mice are described.

Cited by 9 publications

References 39 publications

Soypeptide supplementation attenuates weight loss and improves quality of life among brain cancer patients in a pilot study

Soypeptide supplementation attenuates weight loss and improves quality of life among brain cancer patients in a pilot study

The SWGEDWGEIW from Soybean Peptides Reduce Oxidative Damage-Mediated Apoptosis in PC-12 Cells by Activating SIRT3/FOXO3a Signaling Pathway

The SWGEDWGEIW from Soybean Peptides Reduces Insulin Resistance in 3T3-L1 Adipocytes by Activating p-Akt/GLUT4 Signaling Pathway

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