A Study of the Effect of Carbon Dioxide on the Germination and Outgrowth of Spores of <i>Clostridium butyricum</i> using a Slide Culture Technique

Hambleton, R.; Rigby, G. J.

doi:10.1111/j.1365-2672.1970.tb02248.x

Cited by 13 publications

(4 citation statements)

References 15 publications

(11 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…perfringens spores but inhibited that of B. cereus spores (Figs 1-2). These results are consistent with earlier observations showing that the germination of Clostridium spores was stimulated by 5% carbon dioxide in nitrogen or by bicarbonate (Wynne & Foster 1948;Hambleton et al 1970;Holland et al 1970). The results are also consistent with the observations that the germination of Bacillus spores can be inhibited by 1 atm carbon dioxide (L-alanine-adenosine initiated germination) or by bicarbonate (Cross et al 1973;Enfors & Molin 1978b).…”

Section: Discussionsupporting

confidence: 92%

“…Carbon dioxide stimulates spore germination of several Clostridium spp. (Hambleton & Rigby 1970;Holland et al 1970;King & Gould 1971) and is essential for others (Wynne & Foster 1948;Treadwell et al 1958;King & Gould 1971). With Bacillus spores the absence of carbon dioxide does not affect germination (Wynne & Foster 1948) and high concentrations of sodium bicarbonate or carbon dioxide inhibit germination (Cross el al.…”

mentioning

confidence: 99%

See 1 more Smart Citation

The Influence of High Concentrations of Carbon Dioxide on the Germination of Bacterial Spores

Enfors¹,

Molin²

1978

Journal of Applied Bacteriology

View full text Add to dashboard Cite

The influence of carbon dioxide at 1–55 atm on the germination of Clostridium sporogenes, Clostridium perfringens and Bacillus cereus spores in a complex medium was studied. The germination studies at atmospheric pressure were done in the pH range 5.2–6.7. Controls at the same pH were done in 100% nitrogen. Carbon dioxide at atmospheric pressure (1 atm) inhibited the spore germination of B. cereus spores but strongly enhanced the germination rate of those of the clostridia. Spore germination of Cl. sporogenes and Cl. perfringens was inhibited completely at 10 atm and at 25 atm, respectively. The germination rate in carbon dioxide or nitrogen was generally higher at pH 6.7 than at 5.2–6.0.

show abstract

Section: Discussionsupporting

confidence: 92%

mentioning

confidence: 99%

The Influence of High Concentrations of Carbon Dioxide on the Germination of Bacterial Spores

Enfors¹,

Molin²

1978

Journal of Applied Bacteriology

View full text Add to dashboard Cite

show abstract

“…The apparatus, medium (YPG), spore suspension of Cl. butyricum NCTC 7423 (SSl) and methods for determining the % germination and outgrowth were as described in the preceding paper (Hambleton & Rigby, 1970).…”

Section: Methodsmentioning

confidence: 99%

The Effect of Oxygen on the Germination and Outgrowth of Spores of Clostridium butyricum

Hambleton

Rigby

1970

Journal of Applied Bacteriology

Self Cite

View full text Add to dashboard Cite

Summary. The germination of spores of Clostridium butyricum, the emergence of germ cells and replication of vegetative cells in atmospheres of different oxygen content have been quantitatively investigated by a nutrient agar slide technique. The sensitivity to oxygen of (1) the process of germination, (2) germinated spores or the process of emergence and (3) cellular replication increases progressively.

show abstract

“…There are, however, reports indicating that spore ger-mination of certain species of Clostridia depends on, or is actively potentiated by, the presence of bicarbonate (Holland et al 1969(Holland et al , 1970Hambleton and Rigby 1970;King and Gould 1971). It is not clear whether this stimulating effect applies equally to spores of Bacillus species because of inadequate and conflicting evidence.…”

Section: Introductionmentioning

confidence: 99%

Interfering mechanism of sodium bicarbonate on spore germination of Bacillus stearothermophilus

Cheung

Sun

1998

J Appl Microbiol

View full text Add to dashboard Cite

ND S .Q . S U N. 1998. Spore germination of Bacillus stearothermophilus was progressively inhibited as the concentrations of sodium bicarbonate (NaHCO 3 ) in the germination media increased from 0% to 1·0% (w/v). The inhibitory effect of NaHCO 3 was attributed to the release of HCO − 3 and its alkaline properties, each of which played a different role. At low concentrations (³ 0·3%), the inhibitory effect of NaHCO 3 was mainly due to bicarbonate. As NaHCO 3 increased from 0·3% to higher concentrations, the effect of HCO − 3 reached a plateau while the alkalinating effect became the more dominant inhibitory factor. Fourier transform infrared (FTIR) analysis reveals that sodium bicarbonate reacted with the carboxyl group (1570 cm −1 ) of some acidic amino-acid residues of protein in the spore, leading to a less orientated structure. A shift of two units towards the longer frequency for carboxyl groups indicates that a stronger interaction was formed between the carboxyl group and the Na ¦ ion. The largest ratio of peak height between the absorbance of carboxylate (1570 cm −1 ) and of amide II (1546 cm −1 ) of spores after pretreatment with 0·3% sodium bicarbonate reflects the biggest structural alterations of keratin-like proteins in the spore. The role of NaHCO 3 in enhancing the sporicidal effect of glutaraldehyde is discussed.

show abstract

A Study of the Effect of Carbon Dioxide on the Germination and Outgrowth of Spores of Clostridium butyricum using a Slide Culture Technique

Cited by 13 publications

References 15 publications

The Influence of High Concentrations of Carbon Dioxide on the Germination of Bacterial Spores

The Influence of High Concentrations of Carbon Dioxide on the Germination of Bacterial Spores

The Effect of Oxygen on the Germination and Outgrowth of Spores of Clostridium butyricum

Interfering mechanism of sodium bicarbonate on spore germination of Bacillus stearothermophilus

Contact Info

Product

Resources

About