A study of the chemiluminescence behavior of myoglobin with luminol and its analytical applications

Abstract

A chemiluminescence signal at 425 nm was observed when ferric state myoglobin was mixed with luminol in alkaline medium. Because the signal was remarkably enhanced in the presence of Fe(CN)6(4-), analytical applications were investigated in a flow-injection system. The increase in chemiluminescence was linearly dependent on myoglobin concentration in the range 0.1 to 100 nmol L(-1), and the limit of detection was 0.04 nmol L(-1) with relative standard deviation 3.2% (3 sigma). It was also found that binding of Mb with the ligands CN-, SCN-, and F- significantly inhibited the chemiluminescence reaction. The linear dynamic ranges for the ligands were 1.0-300.0, 0.1-3.0, and 0.5-100.0 nmol L(-1), and the limits of detection (S/N=3) 0.4, 0.04, and 0.2 nmol L(-1), for F-, CN-, and SCN-, respectively. The relative standard deviations were 5.32%, 6.13%, and 3.38% for 0.1 nmol L(-1) CN-, 0.5 nmol L(-1) SCN-, and 1.0 nmol L(-1) F-, respectively. At a flow rate of 2.0 mL min(-1) the assay could be accomplished in 1 min, including sampling and washing. The method has been successfully applied to the determination of myoglobin in human urine and F- in water samples. A possible mechanism of chemiluminescence production by myoglobin and luminol is presented.

Determination of picomole amounts of formaldehyde in air and bio-fluids based on its enhanced myoglobin-luminol chemiluminescence reaction

“…It was previously reported that myoglobin (Mb), which contains a single iron protoporphyrin or haem moiety in the ferric state, namely Mb(Fe(III)), could react with luminol yielding CL emission [11]. Also, in this work, it was first found that the specific binding of Mb(Fe(III)) with formaldehyde could accelerate electron transfer between Mb and luminol, producing a stronger CL signal at 425 nm.…”

Sensitive determination of gentiopicroside in medicine and bio‐fluids using luminol‐myoglobin chemiluminescence combined with flow injection technique

“…[15] In this paper, it was fi rst observed that GPS could remarkably inhibit the CL reaction between luminol and myoglobin. The decrement of CL signal was linear with the logarithm of GPS concentration over the range from 10.0 pg mL −1 to 500.0 ng mL −1 (r 2 = 0.9992) with relative standard deviations of less than 3% (n = 5).…”

Rapid determination of clindamycin in medicine with myoglobin–luminol chemiluminescence system

“…It was previously reported that myoglobin (Mb), which contains a single iron protoporphyrin or heme moiety in the ferric state Mb(Fe III ) reacts with luminol yielding CL emission. This was exploited for the determination of myoglobin [17]. It was also found that ligands such as fluoride, cyanide and thiocyanate binding to the iron ion in the heme structure inhibit the CL reaction.…”

“…It was also found that ligands such as fluoride, cyanide and thiocyanate binding to the iron ion in the heme structure inhibit the CL reaction. This was used for the indirect determination of these ions [17]. In this paper it is described that clindamycin also inhibits the CL reaction between luminol and myoglobin.…”