Determination of picomole amounts of formaldehyde in air and bio-fluids based on its enhanced myoglobin-luminol chemiluminescence reaction

Xie, Xiaofeng; Song, Zhenghua; Shao, Xiaodong

doi:10.1080/03067310600929266

Cited by 7 publications

(1 citation statement)

References 9 publications

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“…It was reported that the binding of ligand to Mb could significantly increase or inhibit luminol−Mb CL reaction. The procedures of determination of nitrite, formaldehyde, gatiloxacin, and clindamycin were established, and the possible interaction mechanisms were given by our group − . In this work, the possible mechanism of luminol−Mb−melamine reaction was investigated using the UV and CL method, and the results are shown in Figure and Table .…”

Section: Applicationsmentioning

confidence: 99%

Subpicogram Determination of Melamine in Milk Products Using a Luminol−Myoglobin Chemiluminescence System

Wang

Chen

Gao

et al. 2009

J. Agric. Food Chem.

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A sensitive chemiluminescence method based on a luminol-myoglobin system is proposed for the determination of melamine in milk products. It was found that the mixed solutions of melamine and myoglobin could react to form a complex on line, which could greatly inhibit the chemiluminescence intensity generated from the reaction between luminol and myoglobin. The decrease in chemiluminescence intensity was proportional to the concentration of melamine, giving a calibration graph linear over the concentration from 10 pg mL(-1) to 50 ng mL(-1) (R(2) = 0.9988) with a detection limit of 3 pg mL(-1) (3sigma). At a flow rate of 2.0 mL min(-1), one analysis cycle, including sampling and washing, could be accomplished in 20 s with a relative standard deviation of <4.0%. The proposed method was applied successfully to the determination of melamine in milk products, and the recovery was from 93.4 to 106.5%. The possible mechanism of luminol-myoglobin-melamine reaction is given.

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Section: Applicationsmentioning

confidence: 99%

Subpicogram Determination of Melamine in Milk Products Using a Luminol−Myoglobin Chemiluminescence System

Wang

Chen

Gao

et al. 2009

J. Agric. Food Chem.

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Picomole‐level Formaldehyde Determination in Gaseous and Beer Samples Using Flow Injection Chemiluminescence Analysis

Tan

Song

2013

J Chinese Chemical Soc

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Based on the linear enhancement of formaldehyde (FA) within 7.0~1000 pmol l -1 on luminol-bovine serum albumin (BSA) chemiluminescence (CL) system, FA determination in air and beer samples using CL with flow injection (FI) was proposed. The detection limit was 2.5 pmol l -1 (3s) and the relative standard deviations were less than 4.5% (n = 7). At a flow rate of 2.0 mL min -1 , a whole analysis from sampling to washing only needed 32 s, offering a sample throughput of 112 h -1 . This proposed method was successfully utilized to determine FA vapor pressure in liquid (121.8 ± 3.8 Pa), FA content in real air sample (8.93 ± 0.03 mg m -3 ), and FA levels in beer (199.5 ± 5.6~225.2 ± 3.5 mg l -1 ), giving determination recoveries from 90.7% to 109.3%. The mechanism of BSA-FA interaction was also investigated, showing FA binding to BSA was a spontaneous process mainly through hydrogen bonding and van der Waals force by FI-CL, with binding constant K of 1.89 × 10 6 l mol -1 and the number of binding sites n of 0.86. Molecular docking analysis further revealed FA could enter into the pocket at subdomain IIA of BSA, with K of 1.71 × 10 5 l mol -1 and DG of -29.68 kJ mol -1 .Keywords: Formaldehyde; Vapor pressure; Real air sample; Beer samples; Bovine serum albumin; Luminol; FI-CL. INTRODUCTIONFormaldehyde (FA), a colorless pungent chemical with high water solubility, is usually released as a fixative for pathologists, a byproduct of combustion and off-gassing of various building products, leading to wide existence in foods, industry and environment.1,2 It has attracted intensive attention in public 3-5 because of its negative impacts on human health. Chemiluminescence (CL) in combination with FI technique has also been favorably utilized in FA determination, 42-44 due to its conspicuous merits in terms of high sensitivity, low limit of detection (LOD), wide dynamic concentration response range, automatability and less reagent consumption. [45][46][47][48][49] However, so far no FI-CL using luminol-bovine serum albumin (BSA) system has been described for FA determination. In this current work, it was found that FA can remarkably enhance CL intensity from luminol-BSA system, and the CL intensity increments were linear with FA concentrations from 7.0 to 1000 pmol l -1 . Based on the good linear enhancement, a simple FI-CL method using luminol-BSA system was first developed to determine FA. RESULTS AND DISCUSSIONThe relative CL intensity-time profile of luminol-BSA-FA system The relative CL intensity-time profile of luminol-BSA-FA system was given in Fig. 4 (I max referred to the maximum CL intensity, and T max referred to the time for the I max ). The studied concentrations of luminol, BSA and FA were 5.0 × 10 -5 , 5.0 × 10 -9 and 3.0 × 10 -10 mol l -1 , respectively. It can be seen that the I max of luminol-BSA system (curve ¡) is 219 with the T max of 4.1 s, and then vanishes in the following 28 s; however in the presence of FA (curve l), the I max of luminol-BSA system strikingly increases about 1.92-fold to 420 with the...

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Detection of formaldehyde vapor using conductive polymer films

Antwi-Boampong

BelBruno

2013

Sensors and Actuators B: Chemical

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Determination of picomole amounts of formaldehyde in air and bio-fluids based on its enhanced myoglobin-luminol chemiluminescence reaction

Cited by 7 publications

References 9 publications

Subpicogram Determination of Melamine in Milk Products Using a Luminol−Myoglobin Chemiluminescence System

Subpicogram Determination of Melamine in Milk Products Using a Luminol−Myoglobin Chemiluminescence System

Picomole‐level Formaldehyde Determination in Gaseous and Beer Samples Using Flow Injection Chemiluminescence Analysis

Detection of formaldehyde vapor using conductive polymer films

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