A study of the attachment phase of phagocytosis by murine macrophages

Allen, Jennifer M.; Cook, G. M. W.

doi:10.1016/0014-4827(70)90629-4

Cited by 29 publications

(2 citation statements)

References 19 publications

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“…A Comment on the Use of Trypsinized Cells in Cytotoxicity Tests.--Trypsin produces various functional and morphological effects on the cell surface (18)(19)(20)(21)(22), some of them even at concentrations as low as 5/zg/ml (19,21). According to Poste (22), and certainly in our own experience, repeated washing and addition of serum (probably to inactivate cell-bound trypsin), are important steps in preserving cell viability after trypsinization.…”

Section: Methodsmentioning

confidence: 99%

Serologically Demonstrable Alloantigens of Mouse Epidermal Cells

Scheid

Boyse

Carswell

et al. 1972

The Journal of Experimental Medicine

269

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Single cells were prepared from mouse tail epidermis by a method which gives high viability counts and so permits their use in cytotoxicity tests. According to tests with standard alloantisera, the antigen phenotype of mouse epidermal cells is H-2+θ+Sk+H-Y+TL-Ly-A-Ly-B,C-PC-. The skin differentiation alloantigen Sk, which is responsible for homograft reactions directed selectively against skin, is expressed also on brain, but not on other cell types; it is present on the transplanted neuroblastoma C1300. Cytotoxicity tests with epidermal cells of H-2 congenic mouse stocks confirm that the Sk locus is not closely linked to H-2. The lymphoid cell differentiation antigen θ also is present on both epidermal cells and brain. Mice frequently retain θ-incompatible or Sk-incompatible skin grafts although they have formed substantial titers of θ or Sk antibody in response to grafting. Male (H-Y) antigen is demonstrable on epidermal cells by cytotoxicity tests with H-Y antibody, as it is also on one other type of cell, spermatozoa.

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Section: Methodsmentioning

confidence: 99%

Serologically Demonstrable Alloantigens of Mouse Epidermal Cells

Scheid

Boyse

Carswell

et al. 1972

The Journal of Experimental Medicine

269

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“…The affinity of mouse peritoneal macrophages to bacteria can be modified by treating the cells with various enzymes attacking the membrane components (Allen and Cook, 1970) . In the case of tubercle bacilli, hydrophobic binding between the bacillary cell wall and the nonpolar molecules of the cell membrane is also considered.…”

mentioning

confidence: 99%

Subcellular and Intercellular Aspects of Tuberculous Infection in Reference to Protection and Sensitization

Kanai¹,

Kondo

1972

JJMSB

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Quantitative Assessment of the Comparative Nanoparticle‐Uptake Efficiency of a Range of Cell Lines

Santos

Varela

Lynch

et al. 2011

Small

242

206

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The mechanism(s) of nanoparticle-cell interactions are still not understood. At present there is little knowledge of the relevant length- and timescales for nanoparticle intracellular entry and localization within cells, or the cell-specificity of nanoparticle uptake and localisation. Here, the effect of particle size on the in-vitro intracellular uptake of model fluorescent carboxyl-modified polystyrene nanoparticles is investigated in various cell lines. A range of micro- and nanoparticles of defined sizes (40 nm to 2 μm) are incubated with a series of cell types, including HeLa and A549 epithelial cells, 1321N1 astrocytes, HCMEC D3 endothelial cells, and murine RAW 264.7 macrophages. Techniques such as confocal microscopy and flow cytometry are used to study particle uptake and subcellular localisation, making significant efforts to ensure reproducibility in a semiquantitative approach. The results indicate that internalization of (nano)particles is highly size-dependent for all cell lines studied, and the kinetics of uptake for the same type of nanoparticle varies in the different cell types. Interestingly, even cells not specialized for phagocytosis are able to internalize the larger nanoparticles. Intracellular uptake of all sizes of particles is observed to be highest in RAW 264.7 cells (a specialized phagocytic cell line) and the lowest in the HeLa cells. These results suggest that (nano)particle uptake might not follow commonly defined size limits for uptake processes, and highlight the variability of uptake kinetics for the same material in different cell types. These conclusions have important implications for the assessment of the safety of nanomaterials and for the potential biomedical applications of nanoparticles.

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A study of the attachment phase of phagocytosis by murine macrophages

Cited by 29 publications

References 19 publications

Serologically Demonstrable Alloantigens of Mouse Epidermal Cells

Serologically Demonstrable Alloantigens of Mouse Epidermal Cells

Subcellular and Intercellular Aspects of Tuberculous Infection in Reference to Protection and Sensitization

Quantitative Assessment of the Comparative Nanoparticle‐Uptake Efficiency of a Range of Cell Lines

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