Serologically Demonstrable Alloantigens of Mouse Epidermal Cells

Scheid, Margrit P.; Boyse, Edward A.; Carswell, Elizabeth A.; Old, Lloyd J.

doi:10.1084/jem.135.4.938

Cited by 269 publications

(53 citation statements)

References 42 publications

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“…These cells simply differentiated into mesenchymal lineages in appropriate media. To identify rat MSCs, many researchers propose that these cells should express a high level of CD29 (receptor for ECM) and CD90 (Thy) (found on the surfaces of thymocytes, neurons, epidermal cells and fibroblasts) (Mirsky et al, 1975;Reif et al, 1964;Scheid et al, 1972;Stern et al, 1973), and lack expression of hematopoietic epitopes (CD45, CD34, CD11b, CD31) (Marcus et al, 2007). Flow cytometry analysis of OBFLCs is in agreement with these previous data.…”

Section: Discussionsupporting

confidence: 76%

Characterization of fibroblast-like cells from the rat olfactory bulb

Soleimani¹,

Nadri²,

Salehi³

et al. 2008

Int. J. Dev. Biol.

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The isolation and characterization of stem cells from an alternative tissue is a subject of intensive investigation. In the present study, we have focused on the characterization of fibroblastic cells in olfactory bulb tissue of the rat. To this end, 4-6 week old rats were killed and their olfactory bulb tissue was dissected out. Olfactory bulb derived fibroblast-like cells were recovered by adhesion to cell culture plastic. The plastic adherent cultivated cells were induced to differentiate along osteoblastic, adipogenic and chondrogenic lineages. Furthermore, the expression of some surface antigens was investigated. We obtained purified cells with spindle shaped morphology in primary culture, which differentiated into mesenchymal lineages. These cells expressed CD29 and CD90 (Thy1.1) surface antigens, but not CD31, CD34 and CD45. Our results indicate that fibroblast-like cells from the olfactory bulb are mesenchymal stem cells in nature. Taken together, our data suggest that olfactory bulb tissue may constitute a new source of mesenchymal stem cells and could be used for the treatment of injury.

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Section: Discussionsupporting

confidence: 76%

Characterization of fibroblast-like cells from the rat olfactory bulb

Soleimani¹,

Nadri²,

Salehi³

et al. 2008

Int. J. Dev. Biol.

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“…Although collagenase treatment did not cause lymphocytes or hepatocytes to become autoimmunogenic, one might speculate that other tissues such as skin or other endocrine glands (24,25) (26). Although few studies on organ or tissue specific antigens have been reported (22,27), more recently these antigens have been invoked to explain the variability of alloimmunogenicity of different organs, e.g., kidney vs. skin (28), and as a potential cause of islet allograft rejection (3).…”

Section: Discussionmentioning

confidence: 99%

Responses of Canine Lymphocytes to Allogeneic and Autologous Islets of Langerhans in Mixed Cell Cultures

Rabinovitch¹,

Fuller²,

Mintz³

et al. 1981

J. Clin. Invest.

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“…Epidermal cells were obtained from the tail skin by trypsinization according to the method of Scheid et al (4) with minor modifications. In one experiment, epidermal cells were separated into fractions enriched and depleted of Fc-positive cells.…”

Section: Cellsmentioning

confidence: 99%

Role of antigen-presenting cells in the development and persistence of contact hypersensitivity.

Ptak¹,

Różycka²,

Askenase³

et al. 1980

The Journal of Experimental Medicine

156

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Three outcomes pertinent to contact sensitivity (CS) follow immunization with various forms of trinitrophenylated (TNP) substrates: (a) specific immunological unresponsiveness for CS is induced when immunization favors activation of splenic suppressor cells. This state is achieved by intravenous injection of trinitrophenyl-conjugated to various types of cells, such as peritoneal exudate cells (PEC). (b) A short-lived or evanescent form of CS is induced when immunization reduces activation of the suppressor circuit. This can be achieved by subcutaneous immunization with trinitrophenyl conjugated to syngeneic PEC, by pretreatment with cyclophosphamide to diminish suppression before intravenous immunization, or by altering the mode of antigen presentation by using TNP-substrate that has undergone phagocytosis. (c) A long-lived form of CS is induced when trinitrophenyl is presented to the immune system on skin cells either by contact skin painting with reactive trinitrophenyl, or by subcutaneous, or even intravenous injection of trinitrophenyl-conjugated epidermal cells. In fact, trinitrophenyl-conjugated epidermal cells induced CS even when the suppressor circuit was activated by intravenous coadministration of TNP-PEC. This implies that antigen presentation on epidermal cells induces sensitized cells that are relatively resistant to suppression. The cell type(s) in the skin that are primarily responsible for this potent form of antigen presentation are most likely Langerhans cells, because they can be concentrated by virtue of their Fc receptors and they are Ia positive. Thus, both the anatomical site where antigen is first encountered by the immune apparatus, as well as the nature of the cells which present the antigen, determine whether a CS response will ensue, as well as whether it will be evanescent or long-lasting.

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Serologically Demonstrable Alloantigens of Mouse Epidermal Cells

Cited by 269 publications

References 42 publications

Characterization of fibroblast-like cells from the rat olfactory bulb

Characterization of fibroblast-like cells from the rat olfactory bulb

Responses of Canine Lymphocytes to Allogeneic and Autologous Islets of Langerhans in Mixed Cell Cultures

Role of antigen-presenting cells in the development and persistence of contact hypersensitivity.

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