Characterization of fibroblast-like cells from the rat olfactory bulb

Soleimani, Masoud; Nadri, Samad; Salehi, Mohammad Saied; Sobhani, Aligholi; Hajarizadeh, Athena

doi:10.1387/ijdb.082710ms

Cited by 4 publications

(5 citation statements)

References 32 publications

(39 reference statements)

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“…Previously, fibroblast‐like cells isolated from adult rat olfactory bulb were shown to have MSC characteristics, as they were capable of differentiating into cells with osteoblastic, adipogenic and chondrogenic properties (Soleimani et al ., ). In this study, we found that neonatal rat olfactory bulb fibroblast‐like cells were able to differentiate into myocardial‐like cells.…”

Section: Discussionmentioning

confidence: 97%

“…MSCs from multiple sources, including olfactory bulb, bone marrow and adipose tissue, can differentiate into cardiomyocytes (Gnecchi et al ., ; Qayyum et al ., ). Olfactory bulb‐derived MSCs have several advantages compared with other MSC sources, including that they are easily enriched, due to their ability to readily adhere to plastic (Soleimani et al ., ), they have a rapid growth rate and take less time to differentiate into cardiogenic cells (within 1 week) compared to MSCs from adipose tissue (about 2 weeks) (Cai et al ., ) and bone marrow (2–4 weeks) (Xing et al ., ; Li et al ., ). In addition, following differentiation, almost all the cardiomyocyte‐like cells from olfactory bulb‐derived MSCs exhibited autonomous contractions which were similar to those of primary cultured rat cardiomyocytes.…”

Section: Discussionmentioning

confidence: 99%

“…To date, the types and functions of stromal cells in the olfactory bulb are still poorly understood. A previous study showed that stromal cells from the olfactory bulb had characteristics of MSCs and could differentiate into osteocytes, chondrocytes and adipocytes (Soleimani et al ., ). MSCs derived from bone marrow, umbilical cord blood and adipose tissue are known to be induced to differentiate into myocardial cells in vitro and in vivo (Cashman et al ., ).…”

Section: Introductionmentioning

confidence: 97%

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Mesenchymal stem cells from rat olfactory bulbs can differentiate into cells with cardiomyocyte characteristics

Huang

Chueh

et al. 2013

J Tissue Eng Regen Med

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Mesenchymal stromal/stem cells (MSCs) are widely distributed in different tissues such as bone marrow, adipose tissues, peripheral blood, umbilical cord and amnionic fluid. Recently, MSC-like cells were also found to exist in rat olfactory bulb and are capable of inducing differentiation into mesenchymal lineages - osteocytes, chondrocytes and adipocytes. However, whether these cells can differentiate into myocardial cells is not known. In this study, we examined whether olfactory bulb-derived MSCs could differentiate into myocardial cells in vitro. Fibroblast-like cells isolated from the olfactory bulb of neonatal rats were grown under four conditions: no treatment; in the presence of growth factors (neuregulin-1, bFGF and forskolin); co-cultured with cardiomyocytes; and co-cultured with cardiomyocytes plus neuregulin-1, bFGF and forskolin. Cell differentiation into myocardial cells was monitored by RT-PCR, light microscopy immunofluorescence, western blot analysis and contractile response to pharmacological treatments. The isolated olfactory bulb-derived fibroblast-like cells expressed CD29, CD44, CD90, CD105, CD166 but not CD34 and CD45, consistent with the characteristics of MSCs. Long cylindical cells that spontaneously contracted were only observed following 7 days of co-culture of MSCs with rat cardiomyocytes plus neuregulin-1, bFGF and forskolin. RT-PCR and western blot analysis indicated that the cylindrical cells expressed myocardial markers, such as Nkx2.5, GATA4, sarcomeric α-actinin, cardiac troponin I, cardiac myosin heavy chain, atrial natriuretic peptide and connexin 43. They also contained sarcomeres and gap junction and were sensitive to pharmacological treatments (adrenal and cholinergic agonists and antagonists). These findings indicate that rat olfactory bulb-derived fibroblast-like cells with MSC characteristics can differentiate into myocardial-like cells.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

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Mesenchymal stem cells from rat olfactory bulbs can differentiate into cells with cardiomyocyte characteristics

Huang

Chueh

et al. 2013

J Tissue Eng Regen Med

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“…Flow cytometry was based on two surface markers, p75 and Thy1 (Au and Roskams, 2003;Soleimani et al, 2008). Cultures were washed three times with PBS and incubated in TE for 5 min to detach the cells.…”

Section: Flow Cytometry: Cell Analysis and Fluorescent Activated Cellmentioning

confidence: 99%

Comparison of bulbar and mucosal olfactory ensheathing cells using FACS and simultaneous antigenic bivariate cell cycle analysis

Kueh

Raisman

Liu

et al. 2011

Glia

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Transplantation of olfactory ensheathing cells (OECs) is a promising route for CNS repair. There have, however, been major discrepancies between the results from different groups. Part of this can be attributed to variations in cell sources and culture protocols. Accurate estimation of the proportions of OECs and their associated fibroblasts (ONFs) and their evolution with time in culture is an essential baseline for establishing the reparative properties of transplants. In this study, we compare the evolution of cultures from the superficial layers of the olfactory bulb with tissue from the olfactory mucosa, both whole and split into lamina propria and epithelial layer. We used FACS based on p75 and Thy1 to provide a robust and objective numerical estimate of the numbers of OECs and ONFs, respectively in the cultures. A novel four color simultaneous antigenic bivariate cell cycle analysis shows that proliferation of OECs is time-limited, and is unable to prevent an overall loss of OECs with time. Overall, the numbers of OECs in the cultures were inversely correlated with the deposition of fibronectin (FN). Further, culture of the cells purified by flow cytometry shows that, whereas the Thy1 population is terminally differentiated, the p75 population from the mucosal samples generates subpopulations with different antigenic phenotypes, including the reappearance of a subpopulation of p75 cells expressing FN. Culturing epithelial samples at high density reveals an unexpected transient stem cell-like population of rapidly proliferating p75 positive cells.

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“…For many years, our work has focused on the isolation and characterization of MSCs from different sources including bone marrow Nadri, 2009), amniotic fluid , olfactory bulb (Soleimani et al, 2008), and trabecular meshwork (Nadri et al, 2013b). MSCs are usually isolated and purified by way of their physical propensity to adhere to the plastic substrate of the cell culture plate.…”

mentioning

confidence: 99%

Isolation and Expansion of Mesenchymal Stem Cells from Human Conjunctival Tissue

Nadri

Yazdani

2015

CP Stem Cell Biology

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Here we describe a simple protocol for the isolation and culture of mesenchymal stem cells (MSCs) from conjunctiva stromal tissue, with applications to stem cell biology and regenerative medicine. This protocol is based on an explant culture protocol for the adhesion and migration of MSCs from tissue biopsy in primary culture and expansion of the MSC population by passaging cells on the surface of plastic culture dishes. Conjunctiva mesenchymal stem cells (CJMSCs) are generally isolated from small biopsies of human conjunctival tissue. Epithelial sheets are carefully loosened and removed by enzyme treatment after 18 hr and placed in fresh medium supplemented with growth factors. When the cells migrating from tissue segments in primary cultures become nearly confluent, the culture is treated with trypsin. A purified population of CJMSCs that retain proliferation and differentiation potential for many passages can be obtained 2 weeks after the initiation of culture. © 2015 by John Wiley & Sons, Inc.

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Characterization of fibroblast-like cells from the rat olfactory bulb

Cited by 4 publications

References 32 publications

Mesenchymal stem cells from rat olfactory bulbs can differentiate into cells with cardiomyocyte characteristics

Mesenchymal stem cells from rat olfactory bulbs can differentiate into cells with cardiomyocyte characteristics

Comparison of bulbar and mucosal olfactory ensheathing cells using FACS and simultaneous antigenic bivariate cell cycle analysis

Isolation and Expansion of Mesenchymal Stem Cells from Human Conjunctival Tissue

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