A study of gastrointestinal cancer‐associated antigen (GICA) in human fetal organs

Raux, Hélène; Labbé, François; Fondanèche, Marie-Claude; Koprowski, Hilary; Burtin, P

doi:10.1002/ijc.2910320309

Cited by 29 publications

(11 citation statements)

References 7 publications

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“…CA 19-9 is not as widely distributed as CA 125 during fetal life. Raux et al (15) detected this antigen in the fetal gastrointestinal tract but not in other fetal organs. The CA 19-9 antigen has been detected only in trace amounts in normal adult digestive organs (15), but the tissue expression and serum concentrations of this antigen are increased in malignant gastrointestinal diseases (9,24).…”

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confidence: 99%

“…Raux et al (15) detected this antigen in the fetal gastrointestinal tract but not in other fetal organs. The CA 19-9 antigen has been detected only in trace amounts in normal adult digestive organs (15), but the tissue expression and serum concentrations of this antigen are increased in malignant gastrointestinal diseases (9,24). For this reason, CA 19-9 has been viewed as an oncofetal antigen for gastrointestinal tract, although it is obviously not organ-or tumor-specific (25,26).…”

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confidence: 99%

“…Although CA 125 and CA 19-9 are known to be expressed in fetal tissues (14)(15)(16), the ontogenesis of these antigens has not been studied. The serum levels of these markers in infancy have neither been thoroughly explored.…”

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confidence: 99%

“…In a 2-y-old boy with a sacrococcygeal yolk sac tumor and elevated serum APF and CA 125 level at diagnosis, a malignant recurrence was associated with elevated APP, but normal CA 125 levels. A 2-y-old boy operated on for a testicular embryonal carcinoma had serum CA 125 and CA 19-9 are expressed in fetal tissues (14)(15)(16), but serum concentrations of these antigens at various gestational ages or during infancy have not been studied earlier.…”

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Tumor Markers CA 125 and CA 19–9 in Cord Blood and During Infancy: Developmental Changes and Use in Pediatric Germ Cell Tumors

et al. 1995

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Tumor Markers CA 125 and CA 19–9 in Cord Blood and During Infancy: Developmental Changes and Use in Pediatric Germ Cell Tumors

et al. 1995

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“…In addition, MAbs G9, (28) B72.3, (29) and 19.9, (30) each of which are known to be reactive with a sialic acid containing carbohydrate epitope, were nonreactive with either the untreated or de-O-acetylated derivative of the normal colon mucin. MAbs B72.3 and G9 were reactive with HCT2 whereas 19.9 was not.…”

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confidence: 98%

Monoclonal Antibody G47 Engineered to be Reactive with Colorectal Tumor Mucin

Gold

2001

Hybridoma and Hybridomics

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We have previously shown that the normal adult colon produces a sialomucin containing the core trisaccharide 1,3 N-acetylgalactosamine. This structure was shown to be the epitope for a polyclonal antiserum that demonstrated colon "specific" activity. Antiserum binding is dependent upon the presence of O-acetylated sialic acids present at high concentrations in normal adult colon tissue. However, O-acetylation of sialic acids is decreased in colorectal cancer. Indeed, approximately 50% of colorectal carcinomas are nonreactive with this antiserum. In the current work, we used a de-O-acetylated, normal colon mucin as immunogen to generate monoclonal antibody (MAb) G47. Untreated normal colon mucins having a high O-acetylated sialic acid content were essentially nonreactive with G47. Removal of O-acetyl groups by saponification generated a reactive mucin derivative while subsequent treatment with neuraminidase abolished reactivity. By immunoperoxidase procedures MAb-G47 was reactive with approximately 85% of colorectal tumors while exhibiting relatively low reactivity with normal colon tissue. Mucins isolated from normal colon had on average less than 10% of the specific epitope as compared with mucins derived from colorectal tumors (p < 0.01). Initial immunohistochemical studies on tumors of noncolonic origin revealed few positive cases. The potential of MAb-G47 to assist in the diagnosis and/or prognosis of colorectal cancer is now being studied.

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Expression of gastrointestinal carcinoma‐associated antigen (GICA) detected in human fetal tissues by monoclonal antibody NS‐19‐9

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Thurin

Svalander

et al. 1984

Intl Journal of Cancer

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The expression of a gastrointestinal carcinoma-associated antigen (GICA), a monosialoganglioside, was investigated in tissues from human fetuses of various gestational ages (10-40 weeks). Mouse monoclonal antibody NS-19-9, generated in mice immunized with SW1116 human colon carcinoma cells, was used along with a second polyclonal antibody to mouse IgG to detect antigen expression as visualized by means of the biotin-avidin-peroxidase assay. Sections of snap-frozen tissues or tissues fixed in 4% formaldehyde, in mercury chloride-formaldehyde, or in Bouin's solution were used. GICA was consistently detected in the mucosal epithelium of the small intestine. In contrast, the mucosal epithelium of the colon-rectum contained no detectable GICA, nor could the antigen be detected biochemically in extracts from the large intestine. GICA was usually found in the epithelium of the larynx, trachea and main bronchi as well as in the conjunctiva, lacrimal and salivary glands, gall bladder and ductus choledochus, and in the epithelium of the renal pelvis of early and mid-gestation fetuses.

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A study of gastrointestinal cancer‐associated antigen (GICA) in human fetal organs

Cited by 29 publications

References 7 publications

Tumor Markers CA 125 and CA 19–9 in Cord Blood and During Infancy: Developmental Changes and Use in Pediatric Germ Cell Tumors

Tumor Markers CA 125 and CA 19–9 in Cord Blood and During Infancy: Developmental Changes and Use in Pediatric Germ Cell Tumors

Monoclonal Antibody G47 Engineered to be Reactive with Colorectal Tumor Mucin

Expression of gastrointestinal carcinoma‐associated antigen (GICA) detected in human fetal tissues by monoclonal antibody NS‐19‐9

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