Expression of gastrointestinal carcinoma‐associated antigen (GICA) detected in human fetal tissues by monoclonal antibody NS‐19‐9

Olding, Lars B.; Thurin, Jan; Svalander, Christian; Koprowski, Hilary

doi:10.1002/ijc.2910340208

Cited by 26 publications

(1 citation statement)

References 13 publications

(11 reference statements)

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“…Low amounts of sialyl Le a antigen are also present on normal epithelial cells (Atkinson et al, 1982;Arends et al, 1983). In the human fetus, this carbohydrate epitope is expressed in epithelial cells of different organs, mainly of endodermal origin (Olding et al, 1984).…”

mentioning

confidence: 99%

Sialyl Lewis(a): a tumor-associated carbohydrate antigen involved in adhesion and metastatic potential of cancer cells.

Ugorski

Laskowska²

2002

Acta Biochim Pol

101

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Neoplastic transformation is often associated with characteristic changes in the expression of the sialyl Lewis(a) and sialyl Lewis(x) antigens, representing typical tumor-associated carbohydrate antigens. High amounts of sialyl Lewis(a) are present in human adenocarcinomas of the colon, pancreas and stomach. A growing amount of data suggests that this carbohydrate structure is the ligand for E-selectin. Sialylated Lewis structures present on the surface of tumor cells are carried by the carbohydrate chains of glycoproteins and glycolipids. There are several lines of evidence showing that sialyl Lewis(a) is responsible for the adhesion of human cancer cells to endothelium. E-selectin present on endothelial cells mediates these interactions. Selectins and their carbohydrate ligands can thus play an important role in the selective homing of tumor cells during metastasis. However, the presence of sialyl Lewis(a) antigen on the surface of tumor cells and their adhesion to E-selectin-expressing cells in in vitro adhesion assay by itself can not be directly related to metastatic properties of all cancer cells.

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mentioning

confidence: 99%

Sialyl Lewis(a): a tumor-associated carbohydrate antigen involved in adhesion and metastatic potential of cancer cells.

Ugorski

Laskowska²

2002

Acta Biochim Pol

101

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ras oncogene p21 as a tumor marker in the cytodiagnosis of gastric and colonic carcinomas

Czerniak

Herz

Koss

et al. 1987

Cancer

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This study was undertaken to determine whether the expression of ras oncogene product p21 can be used as a tumor cell marker of gastric and colonic carcinoma in brush smears. To detect p21 an immunocytochemical assay with RAP-5 monoclonal antibody was used. Benign epithelial gastric cells obtained from normal gastric mucosa or benign gastric lesions reacted negatively in 12 out of 13 cases. Similarly, benign epithelial colonic cells from normal colon or benign colonic lesions were negative for p21 in nine out of ten cases. Weakly positive reaction, confined to a few cell clusters only, was observed in one smear of a benign gastric ulcer and one smear of chronic ulcerative colitis. All 20 smears from colonic carcinoma and all 20 smears of gastric carcinoma contained cells that stained positively for p21, and the degree of tumor differentiation had no impact on the staining pattern. The results recorded in this study show that the immunocytochemical assay for the ras oncogene product may prove to represent a new tool for the cytodiagnosis of gastric and colonic carcinomas.

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Expression of gastrointestinal carcinoma‐associated antigen (GICA) detected in human fetal tissues by monoclonal antibody NS‐19‐9

Olding

Thurin

Svalander

et al. 1984

Intl Journal of Cancer

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The expression of a gastrointestinal carcinoma-associated antigen (GICA), a monosialoganglioside, was investigated in tissues from human fetuses of various gestational ages (10-40 weeks). Mouse monoclonal antibody NS-19-9, generated in mice immunized with SW1116 human colon carcinoma cells, was used along with a second polyclonal antibody to mouse IgG to detect antigen expression as visualized by means of the biotin-avidin-peroxidase assay. Sections of snap-frozen tissues or tissues fixed in 4% formaldehyde, in mercury chloride-formaldehyde, or in Bouin's solution were used. GICA was consistently detected in the mucosal epithelium of the small intestine. In contrast, the mucosal epithelium of the colon-rectum contained no detectable GICA, nor could the antigen be detected biochemically in extracts from the large intestine. GICA was usually found in the epithelium of the larynx, trachea and main bronchi as well as in the conjunctiva, lacrimal and salivary glands, gall bladder and ductus choledochus, and in the epithelium of the renal pelvis of early and mid-gestation fetuses.

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Expression of gastrointestinal carcinoma‐associated antigen (GICA) detected in human fetal tissues by monoclonal antibody NS‐19‐9

Cited by 26 publications

References 13 publications

Sialyl Lewis(a): a tumor-associated carbohydrate antigen involved in adhesion and metastatic potential of cancer cells.

Sialyl Lewis(a): a tumor-associated carbohydrate antigen involved in adhesion and metastatic potential of cancer cells.

ras oncogene p21 as a tumor marker in the cytodiagnosis of gastric and colonic carcinomas

Expression of gastrointestinal carcinoma‐associated antigen (GICA) detected in human fetal tissues by monoclonal antibody NS‐19‐9

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