A study of alkaline phosphatase isoenzyme electrophoresis on cellulose acetate compared with agar, agarose and acrylamide in the presence or absence of triton X-100

Laan, Harry; Diaz, D.; Szakaly, M.

doi:10.1016/0009-8981(79)90449-2

Cited by 8 publications

(1 citation statement)

References 18 publications

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“…To recognize the origin of the different isoenzymes of ALP present in the serum or plasma, different techniques can be used (GADE et al, 2011), such as inhibition by Lphenylalanine (KREISHER et al, 1965), urea (BAHR;WILKINSON, 1967), levamisole (CHAN; KELLEN, 1975), homoarginine (FISHMAN; SIE, 1970), electrophoresis (LAAN et al, 1979) and ion exchange chromatography (BARRAL; COSTA, 1990). According to Matos;Sant'Ana (1996), these methods vary widely in cost and complexity.…”

Section: Introductionmentioning

confidence: 99%

Bone alkaline phosphatase in donkeys obtained by the thermal inactivation method

Aranha¹,

Araújo

Guerra

et al. 2018

AVB

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Article history The bone alkaline phosphatase has clinical importance in pathological orthopedic conditions as a marker of the osteoblastic activity, especially in malignant bone tumors and therapeutic monitoring of osteoporosis and other diseases. The reaction of Roy quantitative and qualitative technique of thermal inactivation was applied to obtain bone alkaline phosphatase isoenzyme of male and female donkeys, without defined breed (SRD) aged from seven to 14 months, subjected to the same diet, divided into two groups, composed of five animals each, being the group 1 (G1) kept in confinement and the Group 2 (G2) in traction activity. Blood samples were collected in eight times with an interval of 90 days, during 21 months. The percentage of thermostable isoenzyme was 35 for the G1 and 32 for the G2. It was concluded that the measurements of bone alkaline phosphatase by the thermal inactivation method showed no changes when compared among the groups, demonstrating that the activity of animal traction exerted by the donkeys do not interfere in bone metabolism of these animals.

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Section: Introductionmentioning

confidence: 99%

Bone alkaline phosphatase in donkeys obtained by the thermal inactivation method

Aranha¹,

Araújo

Guerra

et al. 2018

AVB

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α1 Isoenzyme of alkaline phosphatases clinical importance and value for the detection of liver metastases

Viot

Thyss

Schneider

et al. 2006

Cancer

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Measurement of the α1 (fast liver) fraction of alkaline phosphatases in the serum for 217 cancer patients, 92 patients with nonmalignant hepatic affections and 131 controls, revealed that the α1 fraction offers better global value (94%), sensitivity (96%), and specificity (93%) than γGT or total alkaline phosphatase determinations for the detection of liver metastases during cancer. Initial data from study of the time of appearance of the α1 fraction reveals that this fraction shows up earlier than rises in the γGT or total alkaline phosphatases. Results of a multiparametric study conducted on the α1 fraction and various hepatic enzymatic tests (SGOT, SGPT, GLDH, ALP, γGT) indicate that the α1 fraction used alone is better than any other test or combination of tests for biological detection of liver metastases. As concerns the influence of chemotherapy on the appearance of the α1 fraction, the majority of the drugs used for anticancer chemotherapy do not seem to affect measurement of the α1 ALP fraction. The α1 fast liver fraction of alkaline phosphatases, detected by electrophoresis on cellulose acetate, can be considered one of the best known tests for the detection of liver metastases. Cancer 52:140‐145, 1983.

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Comparative study of gamma glutamyl transferase, alkaline phosphatase and its αl isoenzyme as biological indicators of liver metastases

Viot

Thyss

Viot

et al. 1981

Clinica Chimica Acta

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A study of alkaline phosphatase isoenzyme electrophoresis on cellulose acetate compared with agar, agarose and acrylamide in the presence or absence of triton X-100

Cited by 8 publications

References 18 publications

Bone alkaline phosphatase in donkeys obtained by the thermal inactivation method

Bone alkaline phosphatase in donkeys obtained by the thermal inactivation method

α1 Isoenzyme of alkaline phosphatases clinical importance and value for the detection of liver metastases

Comparative study of gamma glutamyl transferase, alkaline phosphatase and its αl isoenzyme as biological indicators of liver metastases

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