A statistical approach to optimization of fermentative production of poly(γ-glutamic acid) from Bacillus licheniformis NCIM 2324

Bajaj, Ishwar B.; Lele, S. S.; Singhal, Rekha S.

doi:10.1016/j.biortech.2008.06.047

Cited by 67 publications

(46 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The dummy variables, which are not assigned any values, introduce some redundancy required by the statistical procedure (Bajaj et al, 2009;Bankar et al, 2009). …”

Section: Effect Of Initial Ph and Temperaturementioning

confidence: 99%

Optimization of poly-ε-lysine production by Streptomyces noursei NRRL 5126

Bankar

Singhal

2010

Bioresource Technology

View full text Add to dashboard Cite

“…The dummy variables, which are not assigned any values, introduce some redundancy required by the statistical procedure (Bajaj et al, 2009;Bankar et al, 2009). …”

Section: Effect Of Initial Ph and Temperaturementioning

confidence: 99%

Optimization of poly-ε-lysine production by Streptomyces noursei NRRL 5126

Bankar

Singhal

2010

Bioresource Technology

View full text Add to dashboard Cite

“…For the production of PGA, a medium reported by Bajaj et al [18] was used, which contained (g/l) glycerol, 62.4; citric acid, 15.2; L-glutamic acid, 20; ammonium sulfate, 8.0; K 2 HPO 4 , 1; MgSO 4 ·7H 2 O, 0.5; CaCl 2 ·2H 2 O, 0.2; MnSO 4 ·7H 2 O, 0.05. Initial pH of the medium was adjusted to 6.5 by using 2 N NaOH and/or 2 N HCl.…”

Section: Bacterial Strain and Mediummentioning

confidence: 99%

“…Concentration of citric acid was determined by the method described by Marrier and Boulet [22]. Concentration of glutamic acid was determined by HPLC under conditions that were identical to that for quantification of PGA [18].…”

Section: Effect Of Tca Cycle Intermediated On Pga Productionmentioning

confidence: 99%

Enhanced Production of Poly (γ-glutamic acid) from Bacillus licheniformis NCIM 2324 by Using Metabolic Precursors

Bajaj

2008

Appl Biochem Biotechnol

View full text Add to dashboard Cite

The aim of the present work was to study the effect of addition of different amino acids and tricarboxylic acid cycle intermediates as metabolic precursors on the production of poly (gamma-glutamic acid) (PGA) by Bacillus licheniformis NCIM 2324. A maximum yield of 35.75 g/l was obtained with the medium supplemented with 0.5 mM L-glutamine and 10 mM alpha-ketoglutaric acid as compared to 26.12 g/l PGA achieved with the control in the absence of metabolic precursors. Addition of precursors also enhanced the utilization of L-glutamic acid during fermentation.

show abstract

“…In the present study, P. aeruginosa grown and produced EPS at range of pH values. In any microbial fermentation process, pH is one of a critical factor, because medium pH directly influences the physiology of a microorganism by affecting nutrient solubility and uptake, cell membrane morphology, EPS activity and product formation (Bajaj et al, 2009). In this study, EPS production was found to be high at pH 7.5 (6.38 ± 0.381 g/l) and this was found to be optimum (Fig.…”

Section: Resultsmentioning

confidence: 60%

Production of Antibacterial and Larvicidal Extracellular Polysaccharide from Pseudomonas aeruginosa Strain b01 Isolated from Wastewater

Benit¹,

Roslin²

2017

Int.J.Curr.Res.Aca.Rev.

View full text Add to dashboard Cite

Article InfoThe aim of this study is to optimize extracellular polysaccharide (EPS) production from Pseudomonas aeruginosa B01 with potent antibacterial and larvicidal activity. EPS producing P. aeruginosa B01 was isolated from wastewater. Process parameters were optimized to enhance the production of EPS. EPS was fractionated with two-, three-volume of various solvent and antibacterial and larvicidal activities were evaluated. The bacterial cell growth was maximum after 48 h fermentation period, however, EPS production was maximum after 72 h. EPS production was found to be high when this organism was grown in the medium containing 1% maltose, 1% ammonium sulphate and 0.1% Mn 2+ ions. EPS production was maximum at pH 7.5 and at 30 °C. Ethanol precipitated fractions showed high activity than other tested solvents. It was further subjected to Thin Layer Chromatography. EPS showed potent larvicidal activity against mosquito larva and showed potent activity against various pathogenic bacteria.

show abstract

A statistical approach to optimization of fermentative production of poly(γ-glutamic acid) from Bacillus licheniformis NCIM 2324

Cited by 67 publications

References 25 publications

Optimization of poly-ε-lysine production by Streptomyces noursei NRRL 5126

Optimization of poly-ε-lysine production by Streptomyces noursei NRRL 5126

Enhanced Production of Poly (γ-glutamic acid) from Bacillus licheniformis NCIM 2324 by Using Metabolic Precursors

Production of Antibacterial and Larvicidal Extracellular Polysaccharide from Pseudomonas aeruginosa Strain b01 Isolated from Wastewater

Contact Info

Product

Resources

About