Optimization of poly-ε-lysine production by Streptomyces noursei NRRL 5126

The production of ε-poly-L: -lysine (ε-PL) by Streptomyces sp. M-Z18 from glycerol was investigated in a 5-L jar-fermenter. Batch fermentations by Streptomyces sp. M-Z18 at various pH values ranging from 3.5 to 4.5 were studied. Based on the analysis of the time course of specific cell growth rate and specific ε-PL formation rate, a novel two-stage pH control strategy was developed to improve ε-PL production by shifting the culture pH from 3.5 to 3.8 after 36 h of cultivation. By applying the strategy, the maximal ε-PL concentration and productivity had a significant improvement and reached 9.13 g L(-1) and 4.76 g L(-1) day(-1), respectively, compared with those in one-stage pH control process where the pH value is controlled at 3.5 (7.83 g L(-1) and 3.13 g L(-1) day(-1)). Fed-batch fermentation with two-stage pH control strategy was also applied to produce ε-PL; final ε-PL concentration of 30.11 g L(-1) was obtained, being 3.3-fold greater than that of batch fermentation. To our knowledge, it is the first report on production of ε-PL from glycerol in fermenter scale and achievement of high ε-PL production with two-stage pH control strategy.

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“…346 [16], Streptomyces noursei NRRL 5126 [17] and Streptomyces sp. M-Z18 [18] were able to ferment glycerol.…”

mentioning

confidence: 99%

Production of ε-poly-l-lysine using a novel two-stage pH control strategy by Streptomyces sp. M-Z18 from glycerol

Chen

Shu

Liao

et al. 2011

Bioprocess Biosyst Eng

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“…Glycerol and glucose were proven to be the best carbon sources by Shima and Sakai [14] through medium composition optimization experiments in 1981. The positive effect of glycerol as a carbon source in e-PL production was also observed by Bankar and Singhal [15]. However, glycerol as a carbon source for e-PL production has only been used in flask experiments, probably because glycerol as a raw material is more expensive than glucose.…”

mentioning

confidence: 58%

Culture medium containing glucose and glycerol as a mixed carbon source improves ε-poly-l-lysine production by Streptomyces sp. M-Z18

Chen

Ren

Dong

et al. 2011

Bioprocess Biosyst Eng

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To improve the efficiency of ε-poly-L-lysine (ε-PL) production by Streptomyces sp. M-Z18, batch and fed-batch fermentations with glucose and glycerol (co-fermentations) were performed. The batch fermentations showed that the initial ratio of glucose to glycerol plays an important role in glucose/glycerol co-fermentation. The optimal glucose/glycerol weight ratio was 30/30; this resulted in a maximum ε-PL productivity of 5.26 g/L/d. Glucose and glycerol were consumed synergistically during the co-fermentation process, and the length of time during which the substrate was exhausted was significantly shortened compared with the single carbon source fermentation. Under optimized conditions, fed-batch fermentations with glucose and glycerol as a mixed carbon source achieved maximum ε-PL concentration and productivity values of 35.14 g/L and 4.85 g/L/d, respectively. These values were respectively 1.43- and 1.39-, and 1.17- and 1.16-folds higher than those obtained from fermentations with glucose and glycerol as single carbon sources. The present study is the first to suggest that glucose/glycerol co-fermentation may be an efficient strategy for ε-PL production by Streptomyces sp. M-Z18.

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“…As a result, the ε-PL secreted by S. albulus IFO 14147 was increased about ninefold (Shih and Shen 2006b). Similarly, Bankar and Singhal optimized medium components using Placket-Burman design method for ε-PL production with S. noursei NRRL 5126 (Bankar and Singhal 2010). Recently, Chheda and Vernekar used orthogonal array method to determine the optimum medium composition for a novel ε-PL producer Bacillus cereus, and the ε-PL titer increased significantly from 36.29 to 83.49 mg/L (Chheda and Vernekar 2014).…”

Section: Fermentative Production Of ɛ-Pl With Waste Feedstockmentioning

confidence: 94%

“…In addition to Streptomyces sp. M-Z18, S. noursei NRRL 5126 and Streptomyces lydicus USE-11 also used glycerol as the main carbon resource (Bankar and Singhal 2010;Hirohara et al 2007). In another study, Xia et al attempted to use hydrolyzed cane molasses to produce ε-PL.…”

Section: Fermentative Production Of ɛ-Pl With Waste Feedstockmentioning

confidence: 99%

Recent advances in the biotechnological production of microbial poly(ɛ-l-lysine) and understanding of its biosynthetic mechanism

Feng

et al. 2016

Appl Microbiol Biotechnol

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Poly(ɛ-L-lysine) (ɛ-PL) is an unusual biopolymer composed of L-lysine connected between α-carboxyl and ɛ-amino groups. It has been used as a preservative in food and cosmetics industries, drug carrier in medicines, and gene carrier in gene therapy. Modern biotechnology has significantly improved the synthetic efficiency of this novel homopoly(amino acid) on an industrial scale and has expanded its industrial applications. In the latest years, studies have focused on the biotechnological production and understanding the biosynthetic mechanism of microbial ɛ-PL. Herein, this review focuses on the current trends and future perspectives of microbial ɛ-PL. Information on the screening of ɛ-PL-producing strains, fermentative production of ɛ-PL, breeding of high-ɛ-PL-producing strains, genomic data of ɛ-PL-producing strains, biosynthetic mechanism of microbial ɛ-PL, and the control of molecular weight of microbial ɛ-PL is included. This review will contribute to the development of this novel homopoly(amino acid) and serve as a basis of studies on other biopolymers.

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Optimization of poly-ε-lysine production by Streptomyces noursei NRRL 5126

Cited by 44 publications

References 26 publications

Production of ε-poly-l-lysine using a novel two-stage pH control strategy by Streptomyces sp. M-Z18 from glycerol

Production of ε-poly-l-lysine using a novel two-stage pH control strategy by Streptomyces sp. M-Z18 from glycerol

Culture medium containing glucose and glycerol as a mixed carbon source improves ε-poly-l-lysine production by Streptomyces sp. M-Z18

Recent advances in the biotechnological production of microbial poly(ɛ-l-lysine) and understanding of its biosynthetic mechanism

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