Enhanced Production of Poly (γ-glutamic acid) from Bacillus licheniformis NCIM 2324 by Using Metabolic Precursors

Bajaj, Ishwar B.

doi:10.1007/s12010-008-8427-5

Cited by 60 publications

(32 citation statements)

References 29 publications

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“…The highest Lornithine production and growth were obtained with 5:5 of the ratio of KAc and succinic acid. Addition of tricarboxylic acid cycle intermediates also resulted in the increase of production of poly (γ-glutamic acid) (36.9 %) by Bacillus licheniformis [25]. L-ornithine is formed from glutamate as poly (γ-glutamic acid).…”

Section: Effect Of Tricarboxylic Acid Cycle Intermediates On L-ornithmentioning

confidence: 99%

Optimization of Fermentation Conditions of the Engineered Corynebacterium Glutamicum to Enhance L-Ornithine Production by Response Surface Methodology

Lu¹,

Jiang²,

Chen³

et al. 2011

J Biotechnol Biomaterial

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Section: Effect Of Tricarboxylic Acid Cycle Intermediates On L-ornithmentioning

confidence: 99%

Optimization of Fermentation Conditions of the Engineered Corynebacterium Glutamicum to Enhance L-Ornithine Production by Response Surface Methodology

Lu¹,

Jiang²,

Chen³

et al. 2011

J Biotechnol Biomaterial

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“…Interestingly, B. subtilis (chungkookjang) could grow in the medium containing 25% of NaCl, and the broth apparent viscosity decreased with the increase in salt concentration, but the γ-PGA volumetric yield decreased by 29.5% when the NaCl concentration increased from 0.5% to 5% [6]; thus, it is more attractive to understand the regulatory effects of NaCl on γ-PGA production. B. subtilis and Bacillus licheniformis are the most prominent bacteria producing γ-PGA [4][5][6][7][8][9][10]. To the best of our knowledge, the isolation of halotolerant B. licheniformis as a γ-PGA producer has not been reported.…”

Section: Introductionmentioning

confidence: 98%

Isolation of Halotolerant Bacillus licheniformis WX-02 and Regulatory Effects of Sodium Chloride on Yield and Molecular Sizes of Poly-γ-Glutamic Acid

Wei

Chen

2009

Appl Biochem Biotechnol

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A poly-gamma-glutamic acid (gamma-PGA) productive strain, halotolerant bacterium WX-02 was isolated from the saline soil of China (Yingcheng). By physiological, biochemical, and 16S rDNA sequence analysis methods, the strain was identified as Bacillus licheniformis. The effect of NaCl concentration on gamma-PGA production by WX-02 was investigated in modified E (ME) medium. It was found that the gamma-PGA production was salt-inducible, and the highest volumetric yield of gamma-PGA (13.86 g/l) was attained with 8% of NaCl. It was also observed that the molecular size of gamma-PGA decreased when the NaCl concentration increased. This was the first report of isolation and identification of a gamma-PGA productive strain, halotolerant B. licheniformis. This study provided a simple strategy for controlling the yield and molecular size of gamma-PGA by WX-02.

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“…For example, γ-PGAs from Bacillus anthracis and Natrialba aegyptiaca are comprised only of D-glutamic acid or L-glutamic acid, respectively (Hezayen et al, 2001;Scorpio et al, 2007). The molecular weight of γ-PGA also depends on the microorganism and culture conditions used (Bajaj and Singhal, 2009;Wu et al, 2010). Such differences may also affect flocculation behavior.…”

Section: Introductionmentioning

confidence: 99%

Flocculation of Real Sewage Sludge Using Poly-γ-glutamic Acid Produced by <i>Bacillus</i> sp. Isolated from Soil

Liu

Yamashita

Tachibana

et al. 2017

J. Chem. Eng. Japan / JCEJ

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To promote the use of sewage sludge on farmland, a occulant compatible with agricultural application is required. In general, an inorganic occulant such as aluminium sulfate is used at large sewage disposal plants. However, there is concern that aluminium accumulates when it is returned to farmland, and therefore a biodegradable rather than an inorganic occulant is required. To obtain biodegradable occulants produced by microorganisms, the latter were isolated from soils and sewage sludges, and the occulation activity of the culture broths was evaluated. Here, real sewage sludge was used for the evaluation because practical use was regarded as an important consideration. As a result, two strains, V13 and RK14, were selected and identi ed as Bacillus megaterium and Bacillus licheniformis, respectively. It was also found that occulation was caused by poly-γ-glutamic acid (γ-PGA). γ-PGA from RK14 cells had considerably higher molecular weight than commercial γ-PGA, and the enantiomeric composition di ered from that of γ-PGA from other B. licheniformis. To obtain mutants with high γ-PGA productivity, RK14 cells were treated with ethyl methanesulfonate, and a mutant (RK14-46) was selected. The culture medium suitable for γ-PGA production by RK14-46 cells was totally di erent from that by RK-14 cells. The γ-PGA production reached 21.1 g/L when RK14-46 cells were cultured in the optimum semisynthetic medium at 30°C for 2 d. Furthermore, the combination of γ-PGA obtained from RK14-46 cells and chitosan, a cationic occulant, was very e ective for occulation of real sewage sludge.

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Enhanced Production of Poly (γ-glutamic acid) from Bacillus licheniformis NCIM 2324 by Using Metabolic Precursors

Cited by 60 publications

References 29 publications

Optimization of Fermentation Conditions of the Engineered Corynebacterium Glutamicum to Enhance L-Ornithine Production by Response Surface Methodology

Optimization of Fermentation Conditions of the Engineered Corynebacterium Glutamicum to Enhance L-Ornithine Production by Response Surface Methodology

Isolation of Halotolerant Bacillus licheniformis WX-02 and Regulatory Effects of Sodium Chloride on Yield and Molecular Sizes of Poly-γ-Glutamic Acid

Flocculation of Real Sewage Sludge Using Poly-γ-glutamic Acid Produced by <i>Bacillus</i> sp. Isolated from Soil

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