A spike in mechanotransductive adenosine triphosphate release from red blood cells in microfluidic constrictions only occurs with rare donors

Abstract

ObjectiveWan et al (Proc Natl Acad Sci USA, 105, 2008, 16432) demonstrated that RBCs rapidly and transiently release a spike of 300% more ATP shortly downstream from a short microfluidic constriction where the cells experience a sudden increase in shear stress. More recent work by Cinar et al (Proc Natl Acad Sci USA, 112, 2015, 11783), however, yielded no evidence for a similar spike in ATP release downstream of the constriction. Our aim was to determine whether a transient spike in mechanotransduction is the typical response of RBCs to the sudden onset of increased shear.MethodsWe investigate ATP release downstream of a microfluidic constriction for 15 participants using a luciferase‐based photoluminescent assay.ResultsWhile we observe mechanotransductive ATP release from blood drawn from all donors, we find evidence of a spike in ATP concentration after the microfluidic constriction for only 2 of 15 participants. No clear trends in ATP release are found with respect to the magnitude of the applied shear stress, or to the gender, age, or physical activity (Baecke) index of the donor.ConclusionsIn aggregate, all data acquired to date suggest that a spike in mechanotransductive ATP due to a suddenly applied increase in shear stress occurs in blood drawn from only 14% of the population.

Extracellular ATP molecules effects the functional properties of granulocyte plasma membrane

“…В проведенном исследовании изучали функциональные свойства плазмалеммы гранулоцитов, их способность регулировать объем, а также миграционную активность клеток в условиях активации элементов пуринергического сигнального каскада. смоделированная экзогенная нагрузка АтФ соответствовала физиологической концентрации (~10 µМ), которую фиксируют при высвобождении АтФ во время деформационного стресса из клеток крови в сосудах микроциркуляторного русла [12].…”

Dynamics of a lipid vesicle across a microfluidic constriction: how do its membrane properties and microenvironment matter?

“…Further, constriction in human vasculature has an established influence on the clustering of biochemically active particles, including drug delivery agents or activated platelets, having far-reaching consequences in forming blood clots (thrombus) in stenosed blood vessels (Bächer, Schrack & Gekle 2017). Furthermore, constriction-induced elongation of cells is also linked with the mechanical transduction of biological signals, alternatively known as mechanotransduction, in choked flow passages (Wan, Ristenpart & Stone 2008; Mancuso & Ristenpart 2018). From a fundamental fluid dynamic perspective, all these scenarios inevitably feature abrupt constrictions in the flow passage that lead to the rapid convergence of the flow streamlines and thus result in drastic variations in the hydrodynamic shear stress in the exterior medium.…”