Microfluidic models of physiological or pathological flow shear stress for cell biology, disease modeling and drug development

Chen, Huaying; Yu, Zhihang; Bai, Siwei; Lü, Hongfei; Xu, Dong-Ling; Chen, Chang; Liu, Di; Zhu, Yonggang

doi:10.1016/j.trac.2019.06.023

Cited by 44 publications

(33 citation statements)

References 145 publications

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“…However, uncontrolled shear stress may have many adverse effects. Elevated fluid shear stress may trigger cell death and tumor angiogenesis . In a study, Rana et al showed that colorectal carcinoma cell line HCT116 undergo almost no cell death within the first 2 min under continuous fluid shear stress of 8–60.5 dyn cm −2 ; however, tumor cell death rate increases to 60% after 20 h .…”

Section: Discussionmentioning

confidence: 99%

A Circulating Bioreactor Reprograms Cancer Cells Toward a More Mesenchymal Niche

et al. 2020

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Cancer is a complex and heterogeneous disease, and cancer cells dynamically interact with the mechanical microenvironment such as hydrostatic pressure, fluid shear, and interstitial flow. These factors play an essential role in cell fate and circulating tumor cell heterogeneity, and can influence the cellular phenotype. In this study, a peristaltic continuous flow reactor is designed and applied to HCT‐116 colorectal carcinoma cells to mimic the fluid dynamics of circulation. With this intervention, a CD44/CD24‐cell subpopulation emerges, and 100 genes are significantly regulated. The expression of cells at 4 h in the flow reactor is very similar to TGF‐ß treatment, which is an inducer of epithelial–mesenchymal transition. ATF3 and SERPINE1 are significantly upregulated in these groups, suggesting that the mesenchymal transition is induced through this signaling pathway. This flow reactor model is satisfactory on its own to reprogram colorectal cancer cells toward a more mesenchymal niche mimicking circulation of the blood.

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Section: Discussionmentioning

confidence: 99%

A Circulating Bioreactor Reprograms Cancer Cells Toward a More Mesenchymal Niche

et al. 2020

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“…2 It has been demonstrated that shear stress modulates different cellular phenomena such as morphology, proliferation, differentiation, metabolism, and communication. 24 Several research groups have therefore developed dynamic systems that are able to provide shear stress while enhancing oxygen and nutrient diffusion at the same time. A variety of solutions have been adopted to generate cell cultures with medium flow using bioreactors with different configurations, as schematized in Figure 2.…”

Section: Dynamic Lung Models: Fluidic Systemsmentioning

confidence: 99%

Breathing in vitro: Designs and applications of engineered lung models

et al. 2021

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The aim of this review is to provide a systematic design guideline to users, particularly engineers interested in developing and deploying lung models, and biologists seeking to identify a suitable platform for conducting in vitro experiments involving pulmonary cells or tissues. We first discuss the state of the art on lung in vitro models, describing the most simplistic and traditional ones. Then, we analyze in further detail the more complex dynamic engineered systems that either provide mechanical cues, or allow for more predictive exposure studies, or in some cases even both. This is followed by a dedicated section on microchips of the lung. Lastly, we present a critical discussion of the different characteristics of each type of system and the criteria which may help researchers select the most appropriate technology according to their specific requirements. Readers are encouraged to refer to the tables accompanying the different sections where comprehensive and quantitative information on the operating parameters and performance of the different systems reported in the literature is provided.

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“…Microfluidic technology can be applied in cell biology, disease research, and drug development [ 71 , 72 , 73 ]. Microfluidic technology can provide us with a lot of mechanical information (shear stress, constraint, base stiffness) [ 74 ].…”

Section: Research Progress In Experimental Measurement Techniques mentioning

confidence: 99%

Recent Advances on the Model, Measurement Technique, and Application of Single Cell Mechanics

Huang

Dai

Shen

et al. 2020

IJMS

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Since the cell was discovered by humans, it has been an important research subject for researchers. The mechanical response of cells to external stimuli and the biomechanical response inside cells are of great significance for maintaining the life activities of cells. These biomechanical behaviors have wide applications in the fields of disease research and micromanipulation. In order to study the mechanical behavior of single cells, various cell mechanics models have been proposed. In addition, the measurement technologies of single cells have been greatly developed. These models, combined with experimental techniques, can effectively explain the biomechanical behavior and reaction mechanism of cells. In this review, we first introduce the basic concept and biomechanical background of cells, then summarize the research progress of internal force models and experimental techniques in the field of cell mechanics and discuss the latest mechanical models and experimental methods. We summarize the application directions of cell mechanics and put forward the future perspectives of a cell mechanics model.

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Microfluidic models of physiological or pathological flow shear stress for cell biology, disease modeling and drug development

Cited by 44 publications

References 145 publications

A Circulating Bioreactor Reprograms Cancer Cells Toward a More Mesenchymal Niche

A Circulating Bioreactor Reprograms Cancer Cells Toward a More Mesenchymal Niche

Breathing in vitro: Designs and applications of engineered lung models

Recent Advances on the Model, Measurement Technique, and Application of Single Cell Mechanics

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