Extracellular ATP molecules effects the functional properties of granulocyte plasma membrane

Abstract

Extracellular ATP is an auto- and paracrine regulator in the mechanisms of intercellular signaling. It is the trigger starting the purinergic signaling cascade also. The aim of this work to study the effect of extracellular ATP on the functional properties (rigidity, surface potential, adhesive properties and osmoregulatory capabilities of the membrane) of the plasma membrane and the migration activity of granulocytes in experiments in vitro. In the experiment, the granulocyte subpopulation separated from the venous blood of healthy people was used. The experiment samples were incubated with adenosine-5-triphosphate disodium salt trihydrate in concentration 10 мМ that matches of ATP concentration released from blood cells during deformation stress in the microvasculature vessels. The stiffness and surface potential of a cell, the adhesion force between erythrocyte and granulocyte was measured by using the method of atomic force microscopy, tests with hypoosmotic load were performed and the migration activity of granulocytes was studied. As a result of the experiment, a decrease in stiffness and surface potential was found by 53.2% and 32.5 % (р