A spectral imaging array biosensor and its application in detection of leukemia cell

Fang, Xiangyi; Liu, Chunlong; Cheng, Xiaoli; Wang, Yaolin; Yang, Yucong

doi:10.1016/j.snb.2011.02.035

Cited by 13 publications

(12 citation statements)

References 24 publications

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“…The details of the spectral SPRi instrument have been reported previously [21,22]. Briefly, with the incident angle fixed, we synchronously obtained SPR spectrum curves for each Au spot on the array using scanning wavelength in the detection procedure.…”

Section: Instrumental Methodsmentioning

confidence: 99%

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Clenbuterol Assay by Spectral Imaging Surface Plasmon Resonance Biosensor System

Yao

Fang

et al. 2015

Appl Biochem Biotechnol

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To prevent illegal use of clenbuterol and for quality control in the food industry, more efficient and reliable methods for clenbuterol detection are needed. In this study, clenbuterol was detected using a spectral imaging surface plasmon resonance sensor system via two inhibition methods: (1) the target site compensation method, in which anti-clenbuterol antibody was immobilized on the sensor chip as a bioprobe and (2) the solution competition method in which a clenbuterol-BSA conjugate was immobilized on the sensor chip as the bioprobe. The detectable clenbuterol concentration ranged between 6.25 and 100 μg/mL for both methods. The clenbuterol limit of detection for the target site compensation method and solution competition method are estimated to be 6.7 and 4.5 μg/mL, respectively. The proposed methods were successfully applied to the detection of clenbuterol molecules and were found to have high specificity and high-throughput and were label free and operationally convenient.

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Section: Instrumental Methodsmentioning

confidence: 99%

“…However, most SPR biosensors only provide a single sample measurement, which is time consuming and of low efficiency. Previously, we developed and reported a spectral imaging surface plasmon resonance (spectral SPRi) biosensor system [21,22] for multichannel measurement. In this work, we have applied the SPRi for CLEN detection.…”

Section: Introductionmentioning

confidence: 99%

Clenbuterol Assay by Spectral Imaging Surface Plasmon Resonance Biosensor System

Yao

Fang

et al. 2015

Appl Biochem Biotechnol

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“…The process of reactions on the sensor chip was characterized by changes in wavelength resonance. A detailed optical layout was described in our previous publications [23,24].…”

Section: Instrumental Methodsmentioning

confidence: 99%

Spectral surface plasmon resonance imaging for the detection of clenbuterol via three-dimensional immobilization of bioprobes

Yao

Fang

et al. 2015

Analytical Biochemistry

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“…Moreover, in comparison with flow cytometry, the system was cheaper and easier to construct because it did not require cell separation and labelling, and may therefore become a convenient alternative when immunophenotyping acute leukemias [65]. However, the approach has two major drawbacks: the complete loss of morphological parameters, and the theoretical difficulty of developing biosensors that can detect multiple cell surface markers (neither of which hamper the use of flow cytometry).…”

Section: Applications In Oncohematologymentioning

confidence: 99%

Biosensors in Clinical Practice: Focus on Oncohematology

Fracchiolla

Artuso

Cortelezzi

2013

Sensors

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Biosensors are devices that are capable of detecting specific biological analytes and converting their presence or concentration into some electrical, thermal, optical or other signal that can be easily analysed. The first biosensor was designed by Clark and Lyons in 1962 as a means of measuring glucose. Since then, much progress has been made and the applications of biosensors are today potentially boundless. This review is limited to their clinical applications, particularly in the field of oncohematology. Biosensors have recently been developed in order to improve the diagnosis and treatment of patients affected by hematological malignancies, such as the biosensor for assessing the in vitro pre-treatment efficacy of cytarabine in acute myeloid leukemia, and the fluorescence resonance energy transfer-based biosensor for assessing the efficacy of imatinib in chronic myeloid leukemia. The review also considers the challenges and future perspectives of biosensors in clinical practice.

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A spectral imaging array biosensor and its application in detection of leukemia cell

Cited by 13 publications

References 24 publications

Clenbuterol Assay by Spectral Imaging Surface Plasmon Resonance Biosensor System

Clenbuterol Assay by Spectral Imaging Surface Plasmon Resonance Biosensor System

Spectral surface plasmon resonance imaging for the detection of clenbuterol via three-dimensional immobilization of bioprobes

Biosensors in Clinical Practice: Focus on Oncohematology

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