A solo luxI-type gene directs acylhomoserine lactone synthesis and contributes to motility control in the marine sponge symbiont Ruegeria sp. KLH11

Zan, Jindong; Choi, Okhee; Meharena, Hiruy S.; Uhlson, Charis L.; Churchill, Mair E. A.; Hill, Russell T.; Fuqua, Clay

doi:10.1099/mic.0.083956-0

Cited by 21 publications

(23 citation statements)

References 31 publications

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“…Marine Alphaproteobacteria have received little attention as heterologous production hosts and while being a rich source of novel natural products, 148,152 the molecular tools for their modification are generally limited to knockouts. 153,154 Their terrestrial relatives have been successfully assessed for the heterologous expression of natural products, e.g. the lasso peptide brevunsin by Sphingomonas subterranea 155 or a triterpene in Rhodococcus capsulatus.…”

Section: Other Future Marine Cell Factoriesmentioning

confidence: 99%

Marine Proteobacteria as a source of natural products: advances in molecular tools and strategies

et al. 2019

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Section: Other Future Marine Cell Factoriesmentioning

confidence: 99%

Marine Proteobacteria as a source of natural products: advances in molecular tools and strategies

et al. 2019

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“…Recently, a LuxI solo named SscI has been identified in the marine sponge symbiont Ruegeria sp. (30), revealing that similar to DARs, AHLs might fulfill functions other than acting as quorum sensing signal in those bacteria.…”

Section: Discussionmentioning

confidence: 99%

Dialkylresorcinols as bacterial signaling molecules

Brameyer

Kresovic

Bode

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

119

108

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It is well recognized that bacteria communicate via small diffusible molecules, a process termed quorum sensing. The best understood quorum sensing systems are those that use acylated homoserine lactones (AHLs) for communication. The prototype of those systems consists of a LuxI-like AHL synthase and a cognate LuxR receptor that detects the signal. However, many proteobacteria possess LuxR receptors, yet lack any LuxI-type synthase, and thus these receptors are referred to as LuxR orphans or solos. In addition to the wellknown AHLs, little is known about the signaling molecules that are sensed by LuxR solos. Here, we describe a novel cell-cell communication system in the insect and human pathogen Photorhabdus asymbiotica. We identified the LuxR homolog PauR to sense dialkylresorcinols (DARs) and cyclohexanediones (CHDs) instead of AHLs as signals. The DarABC synthesis pathway produces the molecules, and the entire system emerged as important for virulence. Moreover, we have analyzed more than 90 different Photorhabdus strains by HPLC/MS and showed that these DARs and CHDs are specific to the human pathogen P. asymbiotica. On the basis of genomic evidence, 116 other bacterial species are putative DAR producers, among them many human pathogens. Therefore, we discuss the possibility of DARs as novel and widespread bacterial signaling molecules and show that bacterial cell-cell communication goes far beyond AHL signaling in nature.

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“…It has been observed that the marine sponge symbiont Ruegeria sp. harbours, in addition to two pairs of luxR/I homologues, the gene sscI , which encodes a LuxI solo producing an AHL that contributes to motility . One final explanation for the presence of LuxI solos in some bacteria is paracrine signalling.…”

Section: Luxi Solosmentioning

confidence: 99%

“…harbours, in addition to two pairs of luxR/I homologues, the gene sscI,w hich encodes aL uxI solo producing an AHL that contributes to motility. [74] One final explanation for the presence of LuxI solos in some bacteria is paracrine signalling. This phenomenon involves cell-cellc ommunication over much shorter distances than that seen by QS.…”

Section: Luxi Solosmentioning

confidence: 99%

New Vocabulary for Bacterial Communication

et al. 2019

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Quorum sensing (QS) is widely accepted as a procedure that bacteria use to converse. However, prevailing thinking places acyl homoserine lactones (AHLs) at the forefront of this communication pathway in Gram‐negative bacteria. With the advent of high‐throughput genomics and the subsequent influx of bacterial genomes, bioinformatics analysis has determined that the genes encoding AHL biosynthesis, originally discovered to be indispensable for QS (LuxI‐like proteins and homologues), are often absent in QS‐capable bacteria. Instead, the sensing protein (LuxR‐like proteins) is present with an apparent inability to produce any outgoing AHL signal. Recently, several signals for these LuxR solos have been identified. Herein, advances in the field of QS are discussed, with a particular focus on recent research in the field of bacterial cell–cell communication.

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A solo luxI-type gene directs acylhomoserine lactone synthesis and contributes to motility control in the marine sponge symbiont Ruegeria sp. KLH11

Cited by 21 publications

References 31 publications

Marine Proteobacteria as a source of natural products: advances in molecular tools and strategies

Marine Proteobacteria as a source of natural products: advances in molecular tools and strategies

Dialkylresorcinols as bacterial signaling molecules

New Vocabulary for Bacterial Communication

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