Marine Proteobacteria as a source of natural products: advances in molecular tools and strategies

Buijs, Yannick; Bech, Pernille Kjersgaard; Vazquez-Albacete, Dario; Bentzon-Tilia, Mikkel; Sonnenschein, Eva C.; Gram, Lone; Zhang, Sheng-Da

doi:10.1039/c9np00020h

Cited by 44 publications

(33 citation statements)

References 162 publications

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“…While, administration of daidzein significantly reversed the situation with boosted Proteobacteria and Deinococcus-Thermus instead of Bacteroidetes compared with SBM group and made the microbial composition close to the FM group in terms of beta diversity analysis. Although the increased abundance of Proteobacteria is usually found to be associated with many human disease 50,51 , some marine Proteobacteria can produce bioactive compounds with anti-cancer and antibiotic activity 52 . Proteobacteria are the prominent gut microbiota in intestine of fish 53 , and its relative abundance is even up to 90.64% in turbot 54 .…”

Section: Discussionmentioning

confidence: 99%

The protective role of daidzein in intestinal health of turbot (Scophthalmus maximus L.) fed soybean meal-based diets

Liu

et al. 2021

Sci Rep

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Soybean meal-induced enteropathy (SBMIE) is prevalent in aquaculture. The aim of this study is to evaluate the role of daidzein on SBMIE of juvenile turbot (Scophthalmus maximus L.) by feeding with fish meal diet (FM), soybean meal diet (SBM, 40% fish meal protein in FM replaced by soybean meal protein) and daidzein diet (DAID, 40 mg/kg daidzein supplemented to SBM) for 12 weeks. We found that daidzein supplementation elevated the gene expression of anti-inflammatory cytokine TGF-β, decreased gene expression of pro-inflammatory cytokines TNF-α and signal molecules p38, JNK and NF-κB. SBM up-regulated the genes expression related to oxidative stress and apoptosis, but dietary daidzein restored it to the similar level with that in FM group. Moreover, dietary daidzein up-regulated gene expression of tight junction protein, and modified the intestinal microbial profiles with boosted relative abundance of phylum Proteobacteria and Deinococcus–Thermus, genera Sphingomonas and Thermus, species Lactococcus lactis, and decreased abundance of some potential pathogenic bacteria. In conclusion, dietary daidzein could ameliorate SBM-induced intestinal inflammatory response, oxidative stress, mucosal barrier injury and microbiota community disorder of turbot. Moreover, p38, JNK and NF-κB signaling might be involved in the anti-inflammatory process of daidzein, and daidzein itself might act as an antioxidant to resist SBM-induced oxidative damage.

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Section: Discussionmentioning

confidence: 99%

The protective role of daidzein in intestinal health of turbot (Scophthalmus maximus L.) fed soybean meal-based diets

Liu

et al. 2021

Sci Rep

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“…Many marine proteobacterial populations are found to produce valuable natural bioactive compounds like astaxanthin, haliangicin, ecotin, bryostatins etc. and these compounds benefit nutritionally and physiologically to the host animal 32 . Also, the colonization depends on the type of carbon sources supplied.…”

Section: Resultsmentioning

confidence: 99%

Bioaugmentation of biofloc system with enzymatic bacterial strains for high health and production performance of Penaeus indicus

Panigrahi

Esakkiraj

Das

et al. 2021

Sci Rep

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The beneficial effects of two probiotic bacterial strains Marinilactibacillus piezotolerans and Novosphingobium sp. during the culture of Indian white shrimp, Penaeus indicus, under biofloc and clear water system were evaluated. The experimental variation were CW1 (M. piezotolerans in clear water), BFT1 (biofloc + M. piezotolerans), CW2 (Novosphingobium sp. in clear water), BFT2 (biofloc + Novosphingobium sp.) and control (without bacterial strains and biofloc). Growth and survival considerably increased in probiotic bio-augmented treatments. Probiotic incorporation significantly improved water quality, especially ammonia reduction. Microbiota analysis from gut samples taken from different treatments revealed varied microbial population structure among clear water culture, biofloc culture and control. Proteobacteria and Firmicutes were the top phyla observed in the treatments which were significantly higher in bio-augmented systems than the control. Vibrio genera were predominantly observed in control and clear water system compared to that of biofloc systems. Immune genes were significantly altered in response to probiotic gut microbial supplementation than the control. Higher gene expression profile of important immune genes was observed in the biofloc reared shrimps. Expression of digestive enzyme related genes such as trypsin, chymotrypsin, cathepsin L, cathepsin B and alpha amylase were also upregulated significantly in probiotic supplementation especially in the biofloc treatments. Proteomic analysis of hepatopancreas of shrimps from different treatments was carried out by using 2D gel electrophoresis and MALDI-TOF analysis. The proteins were mostly related to growth and stress tolerance. Eukaryotic initiation factor 4E binding protein was expressed in all the groups and it was high in biofloc treated animals followed by animals treated solely with probiotics compared to those of control groups. The results concludes that biofloc already proved as an effective culture method for healthy shrimp production and supplementation of probiotic bacterial strains registered additional benefit for growth, survival, microbial, immunological status of P, indicus culture.

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“…Although Proteobacteria is the most abundant phylum in the ocean, representing 55% of ocean bacteria (Sunagawa et al, 2015), these bacteria are scarcely explored in terms of their biotechnological potential while holding many biosynthetic gene clusters in their genomes, potentially linked with the production of bioactive compounds (Buijs et al, 2019). More than 15% of their genome is dedicated to natural products biosynthesis with structural features that include halogenation, sulfur-containing heterocycles, non-ribosomal peptides and polyketides with unusual biosynthetic pathways (Timmermans et al, 2017).…”

Section: Bacteria and Archaeamentioning

confidence: 99%

“…More than 15% of their genome is dedicated to natural products biosynthesis with structural features that include halogenation, sulfur-containing heterocycles, non-ribosomal peptides and polyketides with unusual biosynthetic pathways (Timmermans et al, 2017). Thus, proteobacteria are promising cell factories and remain an attractive source of new drug leads (Buijs et al, 2019). For example, more than 20 biosynthetic gene clusters are found in a single genome of pigmented Pseudoalteromonas strains (Paulsen et al, 2019), comparable to the prolific phylum Actinobacteria.…”

Section: Bacteria and Archaeamentioning

confidence: 99%

The Essentials of Marine Biotechnology

Rotter

Barbier²,

Bertoni

et al. 2021

Front. Mar. Sci.

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Coastal countries have traditionally relied on the existing marine resources (e.g., fishing, food, transport, recreation, and tourism) as well as tried to support new economic endeavors (ocean energy, desalination for water supply, and seabed mining). Modern societies and lifestyle resulted in an increased demand for dietary diversity, better health and well-being, new biomedicines, natural cosmeceuticals, environmental conservation, and sustainable energy sources. These societal needs stimulated the interest of researchers on the diverse and underexplored marine environments as promising and sustainable sources of biomolecules and biomass, and they are addressed by the emerging field of marine (blue) biotechnology. Blue biotechnology provides opportunities for a wide range of initiatives of commercial interest for the pharmaceutical, biomedical, cosmetic, nutraceutical, food, feed, agricultural, and related industries. This article synthesizes the essence, opportunities, responsibilities, and challenges encountered in marine biotechnology and outlines the attainment and valorization of directly derived or bio-inspired products from marine organisms. First, the concept of bioeconomy is introduced. Then, the diversity of marine bioresources including an overview of the most prominent marine organisms and their potential for biotechnological uses are described. This is followed by introducing methodologies for exploration of these resources and the main use case scenarios in energy, food and feed, agronomy, bioremediation and climate change, cosmeceuticals, bio-inspired materials, healthcare, and well-being sectors. The key aspects in the fields of legislation and funding are provided, with the emphasis on the importance of communication and stakeholder engagement at all levels of biotechnology development. Finally, vital overarching concepts, such as the quadruple helix and Responsible Research and Innovation principle are highlighted as important to follow within the marine biotechnology field. The authors of this review are collaborating under the European Commission-funded Cooperation in Science and Technology (COST) Action Ocean4Biotech – European transdisciplinary networking platform for marine biotechnology and focus the study on the European state of affairs.

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Marine Proteobacteria as a source of natural products: advances in molecular tools and strategies

Abstract: This review covers the recent advances in molecular tools and strategies for studies and use of natural products from marine Proteobacteria.

Cited by 44 publications

References 162 publications

The protective role of daidzein in intestinal health of turbot (Scophthalmus maximus L.) fed soybean meal-based diets

The protective role of daidzein in intestinal health of turbot (Scophthalmus maximus L.) fed soybean meal-based diets

Bioaugmentation of biofloc system with enzymatic bacterial strains for high health and production performance of Penaeus indicus

The Essentials of Marine Biotechnology

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