New Vocabulary for Bacterial Communication

Tobias, Nicholas J.; Brehm, Jannis; Kresovic, Darko; Brameyer, Sophie; Bode, Helge B.; Heermann, Ralf

doi:10.1002/cbic.201900580

Cited by 33 publications

(27 citation statements)

References 108 publications

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“…LuxR is then labeled LuxR solo, and the receptor is called SdiA ( 16 ). For instance, Escherichia coli has the SdiA receptor without being able to produce an auto-inducer ( 17 ). This ability to “listen” to other bacterial species is essential in complex ecosystem like the gut microbiota, where hundreds of different bacteria coexist.…”

Section: Introductionmentioning

confidence: 99%

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Impact of N-Acyl-Homoserine Lactones, Quorum Sensing Molecules, on Gut Immunity

2020

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Among numerous molecules found in the gut ecosystem, quorum sensing (QS) molecules represent an overlooked part that warrants highlighting. QS relies on the release of small molecules (auto-inducers) by bacteria that accumulate in the environment depending on bacterial cell density. These molecules not only are sensed by the microbial community but also interact with host cells and contribute to gut homeostasis. It therefore appears entirely appropriate to highlight the role of these molecules on the immune system in dysbiosis-associated inflammatory conditions where the bacterial populations are imbalanced. Here, we intent to focus on one of the most studied QS molecule family, namely, the type I auto-inducers represented by N- acyl-homoserine lactones (AHL). First described in pathogens such as Pseudomonas aeruginosa , these molecules have also been found in commensals and have been recently described within the complex microbial communities of the mammalian intestinal tract. In this mini-review, we will expound on this emergent field of research. We will first recall evidence on AHL structure, synthesis, receptors, and functions regarding interbacterial communication. Then, we will discuss their interactions with the host and particularly with agents of the innate and adaptive gut mucosa immunity. This will reveal how this new set of molecules, driven by microbial imbalance, can interact with inflammation pathways and could be a potential target in inflammatory bowel disease (IBD). The discovery of the general impact of these compounds on the detection of the bacterial quorum and on the dynamic and immune responses of eukaryotic cells opens up a new field of pathophysiology.

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Section: Introductionmentioning

confidence: 99%

“…strain 5_7_47FAA ( 64 ). In addition, bacteria like E. coli, Enterobacter , or Klebsiella are part of the normal gut microbiota and express, as mentioned above, the receptor SdiA and therefore can sense AHL ( 17 ). One must highlight the fragility of small molecules like AHL.…”

Section: Introductionmentioning

confidence: 99%

Impact of N-Acyl-Homoserine Lactones, Quorum Sensing Molecules, on Gut Immunity

2020

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“…However, provided the subtle differences in LuxI synthase homologies and resulting chemical diversity of produced AHLs 34 , we should also consider that these synthases could instead utilize an acyl-ACP or CoA thioester precursor not available to the heterologous E. coli host, and we are actively exploring cultivation conditions that might induce native AHL production from A. gephyra 55 , 59 . While numerous bacteria have been observed to possess orphaned LuxR-type AHL receptors, a functional solo LuxI synthase without any cognate LuxR receptor also present in the genome has yet to be reported 21 , 23 , 24 , 62 . Although a functional orphaned LuxI-type synthase capable of producing AHLs has been reported from the sponge symbiont Ruegeria sp.…”

Section: Discussionmentioning

confidence: 99%

Identification of a solo acylhomoserine lactone synthase from the myxobacterium Archangium gephyra

Albataineh

Duke

Misra

et al. 2021

Sci Rep

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Considered a key taxon in soil and marine microbial communities, myxobacteria exist as coordinated swarms that utilize a combination of lytic enzymes and specialized metabolites to facilitate predation of microbes. This capacity to produce specialized metabolites and the associated abundance of biosynthetic pathways contained within their genomes have motivated continued drug discovery efforts from myxobacteria. Of all myxobacterial biosynthetic gene clusters deposited in the antiSMASH database, only one putative acylhomoserine lactone (AHL) synthase, agpI, was observed, in genome data from Archangium gephyra. Without an AHL receptor also apparent in the genome of A. gephyra, we sought to determine if AgpI was an uncommon example of an orphaned AHL synthase. Herein we report the bioinformatic assessment of AgpI and discovery of a second AHL synthase from Vitiosangium sp. During axenic cultivation conditions, no detectible AHL metabolites were observed in A. gephyra extracts. However, heterologous expression of each synthase in Escherichia coli provided detectible quantities of 3 AHL signals including 2 known AHLs, C8-AHL and C9-AHL. These results suggest that A. gephyra AHL production is dormant during axenic cultivation. The functional, orphaned AHL synthase, AgpI, is unique to A. gephyra, and its utility to the predatory myxobacterium remains unknown.

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“…While P. fallax showed susceptibility by displaying between 5 and 41 new mass features as a response to different concentrations of DMSO, ethanol, and toluene, C. coralloides only produced 4 new mass features in total as a response to all tested solvents and concentrations. (Figure 6) [42]. It has been shown in manifold research papers that coculture is a productive way of exploiting the biosynthetic potential of microbes [43].…”

Section: Strain New Mass Features On M9 Fex New Mass Features On Gms Fexmentioning

confidence: 99%

“…Ba3, which has been identified as cyclo-(Tyr-Pro) ( Figure S21), is a cyclic dipeptide with antimicrobial activity against gram-negative strains and has so far not been reported in literature for strain B. amyloliquefaciens DSM7, although strains from the B. subtilis group, are generally regarded as producers of cyclic peptides [41,42]. It is produced under various culture conditions: elevated temperature, Mg 2+ -limitation, and two different complex media.…”

Section: Suitability Of Tested Culture Conditions For the Generation mentioning

confidence: 99%

Triaging of Culture Conditions for Enhanced Secondary Metabolite Diversity from Different Bacteria

Schwarz¹,

Lütz²

2020

Preprint

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Over the past decade, the One Strain Many Compounds (OSMAC) approach has been established for silent gene cluster activation and elicitation of secondary metabolite production, but so far the full secondary metabolome of a biosynthetically promising bacterium has not been elucidated yet. Here, we investigate the ability of seven categories of OSMAC conditions to enhance the diversity of new mass features from bacterial strains with little literature coverage but high biosynthetic potential. The strains Bacillus. amyloliquefaciens DSM7, Corallococcus. coralloides DSM2259, Pyxidicoccus. fallax HKI727, Rhodococcus. jostii DSM44719, and Streptomyces. griseochromogenes DSM40499 were selected after genome mining with antiSMASH. After cultivation under OSMAC conditions, the generated extracts were subjected to LC-MS and MZmine analysis to determine new mass features and evaluate the tested culture conditions. 4 predicted compounds, bacillibactin, desferrioxamine B, myxochelin A, and surfactin, were identified and up to 147 new mass features were detected in the generated extracts, which greatly surpasses the number of predicted gene clusters. Among the new mass features are bioactive compounds that were so far unreported for the strains such as cyclo-(Tyr-Pro) from DSM7 and nocardamin from DSM2259. Furthermore, the tested culture conditions were evaluated regarding their suitability for the generation of new mass features from the selected strains and promising new starting points for further screenings are postulated. Especially culture conditions with little prior literature coverage are responsible for the activation of predicted gene clusters

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New Vocabulary for Bacterial Communication

Cited by 33 publications

References 108 publications

Impact of N-Acyl-Homoserine Lactones, Quorum Sensing Molecules, on Gut Immunity

Impact of N-Acyl-Homoserine Lactones, Quorum Sensing Molecules, on Gut Immunity

Identification of a solo acylhomoserine lactone synthase from the myxobacterium Archangium gephyra

Triaging of Culture Conditions for Enhanced Secondary Metabolite Diversity from Different Bacteria

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