A solid-phase fluoroimmunoassay for the quantitation of antibodies to Toxoplasma gondii

Comparison of an enzyme-linked immunoassay and a quantitative indirect fluorescent-antibody test with the conventional indirect fluorescent-antibody test for detecting antibodies to Toxoplasma gondii

“…Applications of ELISA (3,6,9,12,13,15) and QIF (4,5,11,14) to toxoplasmosis serology have been described separately, and commercial applications that exploit these methods are now available to the routine clinical laboratory. This report compares the ELISA and QIF assays with the standard indirect fluorescent-antibody (IFA) test.…”

“…QIF. A QIF assay for antibodies to T. gondii was developed based on the methods of Walls et al 15and others (5,10,11). The fluorometer, samplers, and reciprocating shaker were obtained from International Diagnostic Technology.…”