A smartphone-based three-in-one biosensor for co-detection of SARS-CoV-2 viral RNA, antigen and antibody

Dou, Yanzhi; Su, Jing; Chen, Shixing; Li, Tie; Wang, Lihua; Li, Yiyang; Song, Shiping; Fan, Chunhai

doi:10.1039/d2cc01297a

Cited by 14 publications

(15 citation statements)

References 19 publications

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“…On the other hand, it is fast because the synthesis is done in less than 10 s and does not need RNA extraction and amplification. It is user friendly because its use is limited to mixing the reaction components and the color changes are clear to the naked eye, while new systems such as CRISPR are based on PCR (RT-PCR) and fluorescent reporter or the smartphone-based electrochemical sensing systems need electrochemical detector and biosensor chip. − Simplicity of the new conjugation method with hybridization and annealing without amplification and denaturation steps enabled it to perform in the μPAD.…”

Section: Resultsmentioning

confidence: 99%

Exploitation of N-Gene of SARS-CoV-2 to Develop a New Rapid Assay by ASOs@AuNPs

2022

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A naked-eye (equipment-free), label-free (cost-effective), and RNA extraction-free (to speed up) method for SARS-CoV-2 (as a case study of RNA viruses) detection is developed. Here, the DNA is being used as a template for in situ formation of anisotropic gold nanoparticles (AuNPs) without any chemical modification or DNA labeling. In this study, synthesized AuNPs for the direct detection of N-gene (nucleocapsid phosphoprotein) of SARS-CoV-2 are exploited. To this aim, antisense oligonucleotides (ASOs) with an extra poly guanine tail (G12) were designed. Thus, in the presence of its viral target RNA gene and ASOs@AuNPs-RNA hybridization, there was a red shift in its localized surface plasmon resonance (LSPR), and the intensity of the LSPR peak at 690 nm of throat swab samples was compared to the threshold cycle (Ct) of a reverse-transcriptase real-time polymerase chain reaction (RT-qPCR) (as a gold standard). Results suggested that the plasmonic biosensor can detect a very low amount of SARS-CoV-2 with a detection limit close to RT-qPCR. Simplicity of the new conjugation method with hybridization and annealing without amplification and denaturation steps enabled it to perform in a microfluidic paper-based analytical device.

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Section: Resultsmentioning

confidence: 99%

Exploitation of N-Gene of SARS-CoV-2 to Develop a New Rapid Assay by ASOs@AuNPs

2022

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“…Recently, a monolithic chip utilizes microfluidic chain reactions powered by an on-chip paper pump to implement an eight-step ELISA protocol autonomously [38] , allowing quantitative detection of antibodies against the N protein of SARS-CoV-2 in saliva. In addition, heterogeneous sensing interfaces for different types of analytes can be integrated into a unified microarray, which enables “all-in-one” assays of multiple biomarkers including nucleic acids and proteins [ 19 , 39 ], holding promise for facile yet comprehensive diagnosis.…”

Section: System Integration For Real-world Detection Of Pathogensmentioning

confidence: 99%

“…The development of mobile smart devices (e.g., smartphones) has also facilitated signal processing, data analysis, and generation of readable conclusions. Recently, smart devices with customized apps have been combined with bioassay chips for diverse infectious diseases including COVID-19 [39] , [40] , [41] . Further, the development of high-speed mobile networks (e.g., 5G networks), big data, artificial intelligence (AI) and cloud computing is enabling remote medical observation, analysis, prognosis, and management, which is helpful for patients in resource-restricted settings, and can relieve the burden on hospital infrastructure during pandemics ( Fig.…”

Section: System Integration For Real-world Detection Of Pathogensmentioning

confidence: 99%

Advancing pathogen detection for airborne diseases

Zhai

Wei²,

Wang³

et al. 2023

Fundamental Research

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“…Another multiplexed electrochemical platform was developed by Dou et al for SARS-CoV-2 detection. The smart sensor recognized the S gene RNA, protein, and antibody simultaneously and collects the data wirelessly [ 115 ]. As well as SARS-CoV-2 variations, HIV, Ebola, and Zika viruses were also targeted by multiplexed sensors with smartphone integration [ 25 , 116 , 117 ].…”

Section: Smartphone-based Multiplexed Biosensingmentioning

confidence: 99%

Smartphone-Based Multiplexed Biosensing Tools for Health Monitoring

et al. 2022

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Many emerging technologies have the potential to improve health care by providing more personalized approaches or early diagnostic methods. In this review, we cover smartphone-based multiplexed sensors as affordable and portable sensing platforms for point-of-care devices. Multiplexing has been gaining attention recently for clinical diagnosis considering certain diseases require analysis of complex biological networks instead of single-marker analysis. Smartphones offer tremendous possibilities for on-site detection analysis due to their portability, high accessibility, fast sample processing, and robust imaging capabilities. Straightforward digital analysis and convenient user interfaces support networked health care systems and individualized health monitoring. Detailed biomarker profiling provides fast and accurate analysis for disease diagnosis for limited sample volume collection. Here, multiplexed smartphone-based assays with optical and electrochemical components are covered. Possible wireless or wired communication actuators and portable and wearable sensing integration for various sensing applications are discussed. The crucial features and the weaknesses of these devices are critically evaluated.

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A smartphone-based three-in-one biosensor for co-detection of SARS-CoV-2 viral RNA, antigen and antibody

Abstract: Rapid and comprehensive diagnostic methods are necessary for early identification and monitoring of SARS-CoV-2. Here, we have developed a universal and portable three-in-one biosensor linked to smartphone for co-detection of...

Cited by 14 publications

References 19 publications

Exploitation of N-Gene of SARS-CoV-2 to Develop a New Rapid Assay by ASOs@AuNPs

Exploitation of N-Gene of SARS-CoV-2 to Develop a New Rapid Assay by ASOs@AuNPs

Advancing pathogen detection for airborne diseases

Smartphone-Based Multiplexed Biosensing Tools for Health Monitoring

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