A Slide Smear Technique for the Diagnosis of Laryngotracheitis

Comparison of cultural methods for primary isolation of infectious laryngotracheitis virus from field material

“…Rapid methods of diagnosis of the disease include haemotoxylin and eosin staining of trachéal sections or smears to demonstrate intranuclear inclusions (Pirozok et al, 1953;Armstrong, 1959), immunofluorescence staining of trachéal smears or sections to demonstrate viral antigen (Braune and Gentry, 1965;Wilks and Kogan, 1979), and direct electron microscopy (EM) of negatively stained preparations of trachéal material to show herpesvirus particles (Van Kammen and Spradbrow, 1976). However, cultural methods may still be required in instances where results of rapid tests are inconclusive.…”