Comparison of cultural methods for primary isolation of infectious laryngotracheitis virus from field material

Hughes, Carolyn S.; Jones, Richard C.

doi:10.1080/03079458808436448

Cited by 46 publications

(31 citation statements)

References 12 publications

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“…The third chorioallantoic membrane passage for the six field isolates was titrated in chicken kidney (CK) cells. CK cells were also utilized to expand the USDA reference strain as previously described by Hughes & Jones (1988). The viral plaque assay was performed in the chicken liver tumour cell line LMH (Kawaguchi et al, 1987), as previously described by Devlin et al (2006).…”

Section: Methodsmentioning

confidence: 99%

Pathogenicity and growth characteristics of selected infectious laryngotracheitis virus strains from the United States

et al. 2009

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In a recent study, several US infectious laryngotracheitis virus (ILTV) strains and field isolates were genotyped by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) into nine different genotypes. All of the commercial poultry isolates were identified within genotypes IV, V, and VI. Based on the PCR-RFLP, Group IV isolates were characterized as genetically identical to the chicken embryo origin (CEO) vaccines, Group V as genetically closely related to the CEO vaccines, and Group VI as genetically different to the vaccine strains. The objective of this study was to determine the pathogenicity and growth characteristics of six ILTV commercial poultry isolates as compared with the CEO vaccine. Two isolates representative of PCR-RFLP Groups IV, V, and VI were selected. Differences in disease severity, viral tissue distribution in chickens, and plaque formation ability in cell culture were observed among viral genotypes IV, V, and VI, and between V-A and V-B isolates. Mild respiratory clinical signs were produced by IV-A, IV-B and the CEO vaccine, while VI-A and VI-B isolates produced severe respiratory signs and severe depression, and during the peak of clinical signs both isolates were re-isolated from the conjunctiva, sinus, trachea and thymus. Similarly to Group VI isolates, V-A and V-B produced severe respiratory signs, depression, and were re-isolated from conjunctiva, sinus, and trachea; on cell culture, both isolates produced significant larger plaques than any of the other isolates analysed. Overall, differences in pathogenicity and growth characteristics were observed among genetically closely related US ILTV isolates; however, complete genomes will be necessary to identify molecular determinants linked to the pathogenic viral phenotypes.

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Section: Methodsmentioning

confidence: 99%

Pathogenicity and growth characteristics of selected infectious laryngotracheitis virus strains from the United States

et al. 2009

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“…ILTV has been propagated in a variety of avian cell cultures, such as chicken embryo liver (CEL), chicken embryo kidney (CEK), and chicken kidney cell cultures (CK). The virus causes rounding of the nucleoli, chromatin displacement, increase of refractiveness, swelling of cells, as well as syncytium formation as the result of cytoplasmic fusion (Hughes & Jones, 1988;Garcia et al, 2014). Chicken embryo liver cells are the most susceptible for the primary isolation of the virus from clinical material (Garcia et al, 2014).…”

Section: Laboratory Host Systemsmentioning

confidence: 99%

Epidemiology of Avian Infectious Laryngotracheitis with Special Focus to South America: an update

Parra

Nuñez

Ferreira

2016

Rev. Bras. Cienc. Avic.

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“…Diagnosis of ILT most often is accomplished by virus isolation (Hughes & Jones, 1988), detection of intranuclear inclusion bodies in tissues using histopathology (Seifried, 1931), or detection of antigen in tissues using fluorescent antibody (FA) procedures (Hitchner et a.l, 1977;Ide, 1978;Wilks & Kogan, 1979). Isolation of ILT virus in embryonated eggs or cell cultures is readily accomplished (Hughes & Jones, 1988); however, these procedures are slow and labour-intensive. Histopathology also tends to be a slow and cumbersome procedure, as considerable time and labour are required for fixation, processing and evaluation of tissues.…”

Section: Introductionmentioning

confidence: 99%

Rapid diagnosis of infectious laryngotracheitis using a monoclonal antibody‐based immunoperoxidase procedure

1992

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SUMMARYAn indirect immunoperoxidase (IP) procedure using monoclonal antibody was developed for detection of infectious laryngotracheitis (ILT) virus antigen in frozen tissue sections. This IP procedure was compared with an indirect immunofluorescent antibody (FA) procedure, histopathology and virus isolation for detection of ILT virus in tracheas of experimentally infected chickens. Compared with virus isolation, sensitivity and specificity of IP were 72 and 93%, respectively; sensitivity and specificity of FA were 53 and 90%, respectively. Histopathological detection of ILT virus infection was highly specific (98%), but sensitivity was poor (42%). These findings indicate potential usefulness of the IP procedure for ILT diagnosis.

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Comparison of cultural methods for primary isolation of infectious laryngotracheitis virus from field material

Cited by 46 publications

References 12 publications

Pathogenicity and growth characteristics of selected infectious laryngotracheitis virus strains from the United States

Pathogenicity and growth characteristics of selected infectious laryngotracheitis virus strains from the United States

Epidemiology of Avian Infectious Laryngotracheitis with Special Focus to South America: an update

Rapid diagnosis of infectious laryngotracheitis using a monoclonal antibody‐based immunoperoxidase procedure

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