Rapid diagnosis of infectious laryngotracheitis using a monoclonal antibody‐based immunoperoxidase procedure

Abstract

SUMMARYAn indirect immunoperoxidase (IP) procedure using monoclonal antibody was developed for detection of infectious laryngotracheitis (ILT) virus antigen in frozen tissue sections. This IP procedure was compared with an indirect immunofluorescent antibody (FA) procedure, histopathology and virus isolation for detection of ILT virus in tracheas of experimentally infected chickens. Compared with virus isolation, sensitivity and specificity of IP were 72 and 93%, respectively; sensitivity and specificity of FA were 53 and 90%, respectively. Histopathological detection of ILT virus infection was highly specific (98%), but sensitivity was poor (42%). These findings indicate potential usefulness of the IP procedure for ILT diagnosis.

Enfeksiyöz Laringotraheitis İle Doğal Enfekte Yumurta Tavuklarında Solunum Kanalı Lezyonları Üzerine İmmunohistokimyasal Çalışmalar

“…On the other hand, FA test was found to be as reliable as histopathological methods [18] . However, it needs for fluorescence microscopy has been reported as a negative aspect [9] . Williams et al [19] compared virus isolation, electron microscopy, and PCR methods and they found that most sensitive methods were PCR, virus isolation and electron microscopy, respectively.…”

“…In an experimental study, immunopositive stainings were observed in laryngeal and tracheal tissues, especially in the cytoplasm of epithelial desquamated and unciliated cells such as goblet cells and mucous gland epithelia [36] . Guy et al [9] reported that in their experimental study, they found positive reactions in tracheal mucosal epithelia and desquamated epithelial cells. In this study, immunopositive reactions were observed in the larynx and tracheal epithelium, luminal epithelial cells and gland epithelium.…”

“…Immunohistochemical methods have recently been used to diagnose ILT by many researchers [8,9,11,[32][33][34] . IHC methods have been shown to be helpful for diagnosing ILT when macroscopical and histopathological findings are not possible [35] .…”

“…These inclusion bodies can also be found in syncytial giant cells. It was emphasized that the inclusion bodies were encountered within the first 5 days of the disease and that disappeared with the progression of the sickness [9,10] .…”

Epidemiology of Avian Infectious Laryngotracheitis with Special Focus to South America: an update

“…Lymphocytes, histiocytic, and blood cells migrate into the mucosa and submucosa in 2-3 days (Garcia et al, 2014). Intranuclear inclusion bodies in the tracheal and conjunctival epithelial cells are present for a few days (1-5 dpi) in the early stages of infection, and then disappear due to necrosis and sloughing of the epithelium (Guy et al, 1992).…”