Enfeksiyöz Laringotraheitis İle Doğal Enfekte Yumurta Tavuklarında Solunum Kanalı Lezyonları Üzerine İmmunohistokimyasal Çalışmalar

Yavuz, Orhan; Özdemir, Özcan; Aras, Zeki; Terzi, Funda

doi:10.9775/kvfd.2017.18805

Cited by 2 publications

(4 citation statements)

References 14 publications

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“…In the present study, the immunohistochemical analysis was showed a high sensitivity through the detection of immunoreactivity and the distribution of ILTV antigen in the syncytial cells, inflammatory cells and epithelial cells of larynx, trachea and lung is used to confirm the occurrence of the disease allowing visualization of viral antigen distributed in different tissues cannot be predicted by PCR as mentioned by Fuchs et al (7). Highest IHC positivity has always been found in the trachea and lung as reported by Yavuz et al (34).…”

Section: Discussionsupporting

confidence: 53%

“…We consider the anatomopathological examination as the primary diagnostic tool and plays an important role in the diagnosis of ILTV infection, particularly in the acute phase of the viral infection by observation of multinucleated syncytial cells containing intranuclear eosinophilic inclusion bodies, a pathognomonic finding for ILTV infection (23,34).…”

Section: Discussionmentioning

confidence: 99%

“…Twenty samples of lung and trachea from different farms that showed severe clinical signs and histopathological lesion score were applied for immunohistochemistry. All the immunohistochemical procedures were performed as previously described by Yavuz et al (34). Tissue sections of lung and trachea were incubated overnight with rabbit polyclonal anti-ILTV-gE antibody diluted 1:400 (Cat.no.…”

Section: Immunohistochemical Analysismentioning

confidence: 99%

“…Gross lesions of ILT can be observed in the larynx and trachea as mucoid or bloody casts obstructing the laryngeal and tracheal lumina, conjunctivitis, swelling of the infraorbital sinuses, and nasal discharge (22). Other lesions are observed in the lungs including congestion and airsacculitis (34).…”

Section: Introductionmentioning

confidence: 99%

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Anatomopathological, ultrastructural, immunohistochemical and molecular characterization of infectious laryngotracheitis outbreaks in poultry farms in Egypt (2018–2020)

El-Saied¹,

El-Mahdy²,

Sakr³

et al. 2021

BJVP

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Infectious laryngotracheitis (ILT) is a severe respiratory disease, which causes high morbidity and mortality in affected birds. In our study, ILT were reported in 42 farms from nine governates over two years (2018–2020) that showed clinical signs of ILT including dyspnea, blood expectoration of, excessive lacrimation, rattling, conjunctivitis. The disease affected different chicken breeds and age groups despite vaccination with licensed and commonly used vaccines. Samples of larynx, trachea, lungs and air sacs were examined and collected for histopathological, ultrastructural, immunohistochemical examination and molecular detection. Gross examination of laryngeal and tracheal lumen revealed different types of exudate varied from catarrhal to fibrinonecotric, also pneumonia and airsacculitis were detected. Histopathological examination showed different alternation in larynx, trachea, lung and air sac as characteristic syncytial cells containing intranuclear inclusion body hanged in fibrinoheterphilic exudate that precent in laryngeal, tracheal, bronchial and parabronchial lumen and air sacs. Tracheal lesion scoring system was used to categorize the severity of lesion in different governates. Tracheal lesion score showed that 6.02%, 26.5%, 43.3% of the birds exhibited mild, moderate, and severe changes, respectively, while 24.18% of the birds exhibited very severe changes. Furthermore, severe cases were related to the Qalyubia , Fayoum then Sharkia Governorate. Moreover, immunohistochemistry was used to detect viral particles in syncytial cells, inflammatory cells beside epithelium of trachea and lung. Transmission electron microscopy enabled the detection of virus particles and demonstrated that heterophils could be infected. PCR targeting a region in the thymidine kinase gene and glycoprotein gJ gene confirmed the presence of infectious laryngotracheitis ILT virus-specific DNA. In conclusion, anatomopathological, immunohistochemical, molecular and ultrastructural findings showed increased of ILTV severity in Egypt. Larynx, trachea, lungs and air sac should be collected and examined that aid in diagnosis. Importance of good biosecurity level to be considered.

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Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Section: Immunohistochemical Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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