A single amino acid substitution controls DAF-dependent phenotype of echovirus 11 in rhabdomyosarcoma cells

Novoselov, A. V.; Rezaykin, A.V.; Sergeev, A.G.; Fadeyev, F. A.; Grigoryeva, Julia V.; Sokolova, Zoya I.

doi:10.1016/j.virusres.2012.03.007

Cited by 9 publications

(9 citation statements)

References 33 publications

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“…The identification of a single CV-B6 amino acid that is most likely responsible for the lytic phenotype, more efficient progeny production and DAF-usage is in line with previous reports that have shown strong effects for single or few amino acid substitutions on the receptor usage, cell and tissue tropism and pathogenesis (Al-Hello et al, 2005Caggana et al, 1993;Chua et al, 2008;Cifuente et al, 2011;Gullberg et al, 2010;Halim and Ramsingh, 2000;Kim and Racaniello, 2007;Knowlton et al, 1996;Novoselov et al, 2012;Paananen et al, 2013;Pan et al, 2011;Pelletier et al, 1998;Polacek et al, 2005;Ramsingh and Collins, 1995;Ramsingh et al, 1997;Schmidtke et al, 2000). A phenomenon similar to our CV-B6 model has been described previously using CV-B2-Ohio strain that induces persistent non-cytolytic infection in CAR-deficient RD-cells.…”

Section: Discussionsupporting

confidence: 84%

Enterovirus strain and type-specific differences in growth kinetics and virus-induced cell destruction in human pancreatic duct epithelial HPDE cells

et al. 2015

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a b s t r a c tEnterovirus infections have been suspected to be involved in the development of type 1 diabetes. However, the pathogenetic mechanism of enterovirus-induced type 1 diabetes is not known. Pancreatic ductal cells are closely associated with pancreatic islets. Therefore, enterovirus infections in ductal cells may also affect beta-cells and be involved in the induction of type 1 diabetes. The aim of this study was to assess the ability of different enterovirus strains to infect, replicate and produce cytopathic effect in human pancreatic ductal cells. Furthermore, the viral factors that affect these capabilities were studied.The pancreatic ductal cells were highly susceptible to enterovirus infections. Both viral growth and cytolysis were detected for several enterovirus serotypes. However, the viral growth and capability to induce cytopathic effect (cpe) did not correlate completely. Some of the virus strains replicated in ductal cells without apparent cpe. Furthermore, there were strain-specific differences in the growth kinetics and the ability to cause cpe within some serotypes. Viral adaptation experiments were carried out to study the potential genetic determinants behind these phenotypic differences. The blind-passage of non-lytic CV-B6-Schmitt strain in HPDE-cells resulted in lytic phenotype and increased progeny production. This was associated with the substitution of a single amino acid (K257E) in the virus capsid protein VP1 and the viral ability to use decay accelerating factor (DAF) as a receptor.This study demonstrates considerable plasticity in the cell tropism, receptor usage and cytolytic properties of enteroviruses and underlines the strong effect of single or few amino acid substitutions in cell tropism and lytic capabilities of a given enterovirus. Since ductal cells are anatomically close to pancreatic islets, the capability of enteroviruses to infect and destroy pancreatic ductal cells may also implicate in respect to enterovirus induced type 1 diabetes. In addition, the capability for rapid adaptation to different cell types suggests that, on occasion, enterovirus strains with different pathogenetic properties may arise from less pathogenic ancestors.

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Section: Discussionsupporting

confidence: 84%

Enterovirus strain and type-specific differences in growth kinetics and virus-induced cell destruction in human pancreatic duct epithelial HPDE cells

et al. 2015

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“…Furthermore, analysis of mutations in the complete VP1 region of sub-genotype A1 among AFP and HFMD cases revealed three significant nucleotide mutations causing three corresponding amino acid mutations. As the VP1 region plays an indispensable role in mediating receptor binding and changes in the receptor might directly affect tissue tropism to produce different clinical symptoms 37,38 , these mutations might change some structure or function of the VP1 protein.…”

Section: Discussionmentioning

confidence: 99%

Multiple genotypes of Echovirus 11 circulated in mainland China between 1994 and 2017

Yan

Chen

et al. 2019

Sci Rep

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Echovirus 11 (E-11) is one of the most frequently isolated enteroviruses causing meningitis and other diseases such as hand, foot, and mouth disease (HFMD) and acute flaccid paralysis (AFP). Fifty-nine newly determined E-11 VP1 sequences from the China AFP and HFMD surveillance network and 500 E-11 VP1 sequences obtained from the GenBank database, which were associated with 12 categories of diseases, were screened for phylogenetic analysis. Based on the standard method of genotype classification, E-11 strains circulated worldwide were reclassified into six genotypes as A, B, C, D, E, and F, in which genotype F is newly divided, and genotypes A and C are further divided into A1–5 and C1–4 by this research, whereas genotype D was still divided into D1–5 as in a previous study of Oberste et al . Sub-genotype A1 was the predominant sub-genotype in mainland China between 2008–2017, whereas sub-genotype D5 was the predominant sub-genotype circulated outside China from 1998–2014. However, genotype and sub-genotype spectra showed statistical significance among AFP and HFMD cases (χ 2 = 60.86, P < 0.001), suggesting that different genotypes might have a tendency to cause different diseases. Strengthening the surveillance of E-11 might provide further information about pathogenic evolution or specific nucleotide mutation associated with different clinical diseases.

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“…The fact that E7, E13 and E19 have been documented to all use the same cell surface receptor (Decay-Accelerating Factor [DAF]) for entry into susceptible cells 2,29,30 , the differing assay results for the three EVs suggest inhibition might not have occurred at entry. However, if their footprints 31 on the receptor differ or if alternate cell surface receptors 32,33 exist for one or more of the Echoviruses analysed in this study, then it is possible that the observed phenotype might be due to attachment or entry inhibition.…”

Section: Na-not Active; Si-selective Index; Ic 50 -50% Inhibitory Conmentioning

confidence: 95%

Enterovirus inhibiting activities of two lupane triterpenoids and anthraquinones from senna siamea stem bark against three serotypes of echovirus

Ogbole¹,

Akinleye²,

Faleye³

et al. 2019

actapharm

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Echovirus 7, 13 and 19 are part of the diseases-causing enteroviruses identified in Nigeria. Presently, no treatment modality is clinically available against these enteric viruses. Herein, we investigated the ability of two anthraquinones (physcion and chrysophanol) and two lupane triterpenoids (betulinic acid and lupeol), isolated from the stem bark of Senna siamea, to reduce the viral-induced cytopathic effect on rhabdomyosarcoma cells using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5diphenyltetrazolium bromide) colorimetric method. Viral-induced CPE by E7 and E19 was inhibited in the presence of all tested compounds, E13 was resistant to all the compounds except betulinic acid. Physcion was the most active with IC 50 of 0.42 and 0.33 μg/mL on E7 and E19, respectively. We concluded that these compounds from Senna siamea possess anti-enteroviral activities and betulinic acid may represent a potential therapeutic agent to control E7, E13, and E19 infections, especially due its ability to inhibit CPE caused by the impervious E13.

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A single amino acid substitution controls DAF-dependent phenotype of echovirus 11 in rhabdomyosarcoma cells

Cited by 9 publications

References 33 publications

Enterovirus strain and type-specific differences in growth kinetics and virus-induced cell destruction in human pancreatic duct epithelial HPDE cells

Enterovirus strain and type-specific differences in growth kinetics and virus-induced cell destruction in human pancreatic duct epithelial HPDE cells

Multiple genotypes of Echovirus 11 circulated in mainland China between 1994 and 2017

Enterovirus inhibiting activities of two lupane triterpenoids and anthraquinones from senna siamea stem bark against three serotypes of echovirus

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