A Simplified Approach to the Phage Typing of Staphylococcus Aureus

Wolfson, Stanley W.; Ghitter, Leah R.

doi:10.1093/ajcp/34.1_ts.92

Cited by 5 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…B. Wilson, University of Wisconsin, Madison. The phages were dispensed with a Sylvana Accu-Drop apparatus and Sylvana cartridges equipped with 27-gauge needles (Wolfson and Ghitter, 1960).…”

Section: Methodsmentioning

confidence: 99%

Bacteriophage Types and Antibiotic Sensitivity of Staphylococci from Bovine Milk and Human Nares1

Jones¹,

Bennett²

1965

Applied Microbiology

View full text Add to dashboard Cite

AND F. W. BENNETT. Bacteriophage types and antibiotic sensitivity of staphylococci from bovine milk and human nares. Appl. Microbiol. 13:725-731. 1965.-The number, phage types, and antibiotic sensitivity of coagulase-positive staphylococci from grade A raw milk samples produced on 40 farms in the Athens, Ga., milkshed were determined. Counts of mannitol-positive staphylococci in milk ranged from 100 to 3,580 per milliliter, with an arithmetic mean of 1,047. Examination of the nares of 48 dairymen on 34 of the farms also revealed that 13 (27%) were carriers of coagulase-positive staphylococci. Isolates from milk (412) and from nares (39) were tested against the Coles, Seto-Wilson, and International phage sets and 87, 68, and 56%, respectively, proved typable. Nine isolates were not typable. Each of the 33 phages used lysed one or more of the isolates. Staphylococcal phage types per milk sample ranged from 0 to 5, 0 to 7, and 0 to 8, with arithmetic means of 1.9, 2.3, and 2.3, respectively. Of the 13 narial carriers, 7 harbored staphylococci of one or more of the same phage types as those isolated from the milk at the respective farms. Randomly selected isolates were tested against high and low concentrations of 12 common antibiotics. All were either moderately sensitive or resistant to polymixin B. Over 30% were moderately sensitive or resistant to dihydrostreptomycin and penicillin individually. With but few exceptions, all isolates were sensitive to chlortetracycline, bacitracin, carbomycin, chloramphenicol, erythromycin, neomycin, novobiocin, oxytetracycline, and tetracycline individually.

show abstract

Section: Methodsmentioning

confidence: 99%

Bacteriophage Types and Antibiotic Sensitivity of Staphylococci from Bovine Milk and Human Nares1

Jones¹,

Bennett²

1965

Applied Microbiology

View full text Add to dashboard Cite

show abstract

“…One of the most useful devices for multiple inoculation, recently reviewed by Hartman (1), is the Accu-Drop multidropper (2) (Scientific Products Co., Evanston, Ill.). This instrument allows bacteriophages or bacteriocins to be inoculated onto bacterial lawns without needleto-agar contact.…”

mentioning

confidence: 99%

Improved Bacteriophage-Bacteriocin Applicator

Farmer

1970

Applied Microbiology

View full text Add to dashboard Cite

show abstract

Bacteriophage Types and Antibiotic Sensitivity of Staphylococci from Bovine Milk and Human Nares

Jones

Bennett

1965

Appl Microbiol

View full text Add to dashboard Cite

The number, phage types, and antibiotic sensitivity of coagulase-positive staphylococci from grade A raw milk samples produced on 40 farms in the Athens, Ga., milkshed were determined. Counts of mannitol-positive staphylococci in milk ranged from 100 to 3,580 per milliliter, with an arithmetic mean of 1,047. Examination of the nares of 48 dairymen on 34 of the farms also revealed that 13 (27%) were carriers of coagulase-positive staphylococci. Isolates from milk (412) and from nares (39) were tested against the Coles, Seto-Wilson, and International phage sets and 87, 68, and 56%, respectively, proved typable. Nine isolates were not typable. Each of the 33 phages used lysed one or more of the isolates. Staphylococcal phage types per milk sample ranged from 0 to 5, 0 to 7, and 0 to 8, with arithmetic means of 1.9, 2.3, and 2.3, respectively. Of the 13 narial carriers, 7 harbored staphylococci of one or more of the same phage types as those isolated from the milk at the respective farms. Randomly selected isolates were tested against high and low concentrations of 12 common antibiotics. All were either moderately sensitive or resistant to polymixin B. Over 30% were moderately sensitive or resistant to dihydrostreptomycin and penicillin individually. With but few exceptions, all isolates were sensitive to chlortetracycline, bacitracin, carbomycin, chloramphenicol, erythromycin, neomycin, novobiocin, oxytetracycline, and tetracycline individually.

show abstract

A Simplified Approach to the Phage Typing of Staphylococcus Aureus

Cited by 5 publications

References 0 publications

Bacteriophage Types and Antibiotic Sensitivity of Staphylococci from Bovine Milk and Human Nares1

Bacteriophage Types and Antibiotic Sensitivity of Staphylococci from Bovine Milk and Human Nares1

Improved Bacteriophage-Bacteriocin Applicator

Bacteriophage Types and Antibiotic Sensitivity of Staphylococci from Bovine Milk and Human Nares

Contact Info

Product

Resources

About