Improved Bacteriophage-Bacteriocin Applicator

Farmer, J. J.

doi:10.1128/aem.20.3.517-518.1970

Cited by 5 publications

(2 citation statements)

References 1 publication

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“…The bacteriocins (plus one control containing TSB and mitomycin) were then applied to each indicator strain plus a control plate. The bacteriocin lysates can be applied manually with disposable tuberculin syringes; however, 23 bacteriocins and one control can be added simultaneously with the ACCU-DROP bacteriophage-bacteriocin applicator (Sylvana Corp., Millburn, N.J.) as modified by Farmer (6). There was considerable time saving and convenience when the latter method was used.…”

Section: Methodsmentioning

confidence: 99%

Epidemiological Differentiation of Serratia marcescens: Typing by Bacteriocin Production1

Farmer¹

1972

Applied Microbiology

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A new method for comparing and differentiating strains of S. marcescens is described which has proved useful in determining the epidemiology of hospital infections. Strains were grown in Trypticase soy broth, and bacteriocin production was induced with mitomycin C for 5 hr. The bacteriocin lysates were then spotted onto nine standard indicator strains, which were chosen with the aid of computer analysis from the 118 indicators tested. After 24 hr at 37 C, zones of inhibition due to bacteriocins were recorded. One hundred twentynine strains were differentiated into 72 different bacteriocin production patterns, but 11 strains were nontypable. None of the 45 other strains of Enterobacteriaceae produced bacteriocins. Bacteriocin production was a stable epidemiological marker. Colonial mutants always had identical patterns, as did the same strain which has passed from patient to patient through cross-infection. The new technique does not require any specialized equipment and can be used in laboratories with limited budgets. The applications of the new method in cross-infection studies and as a supplement to serological typing are discussed.

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Section: Methodsmentioning

confidence: 99%

Epidemiological Differentiation of Serratia marcescens: Typing by Bacteriocin Production1

Farmer¹

1972

Applied Microbiology

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“…2. IsolatesA and B have identical lysis patterns against12 bacteriophages, but isolate C has a different pattern.…”

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Differentiation of Proteus mirabilis by bacteriophage typing and the Dienes reaction

Hickman¹,

Farmer²

1976

J Clin Microbiol

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A provisional typing schema based on sensitivity to 23 bacteriophages has been established for Proteus mirabilis. Seventy-three bacteriophages were isolated on strains of P. mirabilis (64), P. vulgaris (1), P. morganii (7), and P. rettgeri (1), but those isolated on P. mirabilis were the most useful in differentiating other strains of . mirabilis. From the 73 phages studied, the best 23 were chosen by computer analysis for the provisional system, which was then used to study P. mirabilis infections in a 500-bed general hospital. All patient isolates for 19 months were saved and then compared by bacteriophage typing and the Dienes reaction in a retrospective study. There was evidence for only three instances of cross-infection or -colonization during this time. Bacteriophage typing was very sensitive in differentiating strains, since 200 strains were differentiated into 113 different lysis patterns and 94% were typable. The Dienes reaction was useful at times but often gave reactions that were difficult to read or that changed when the tests were repeated. The bacteriophages described by Schmidt and Jeffries were also evaluated and proved useful in combination with ours. The value of bacteriophage typing was clearly established, and work toward a standardized schema for P. mirabilis should continue.

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Nursery Outbreak of Severe Diarrhœa Due to Multiple Strains of Pseudomonas Æruginosa

et al. 1972

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Improved Bacteriophage-Bacteriocin Applicator

Cited by 5 publications

References 1 publication

Epidemiological Differentiation of Serratia marcescens: Typing by Bacteriocin Production1

Epidemiological Differentiation of Serratia marcescens: Typing by Bacteriocin Production1

Differentiation of Proteus mirabilis by bacteriophage typing and the Dienes reaction

Nursery Outbreak of Severe Diarrhœa Due to Multiple Strains of Pseudomonas Æruginosa

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