A simple UV-Vis spectrophotometric method for determination of β-carotene content in raw carrot, sweet potato and supplemented chicken meat nuggets

Biswas, Ashim Kumar; Sahoo, J.; Chatli, Manish Kumar

doi:10.1016/j.lwt.2011.03.017

Cited by 136 publications

(88 citation statements)

References 12 publications

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“…As the methods for the determination of β-carotene, spectrophotometric (Biswas et al 2011;Schierle et al 2002) and chromatographic methods, both in normal and reversed phase, were used (Brabcova et al 2013;Burri et al 1997;Guedes De Pinho et al 2001;Hart and Scott 1995;Khachik et al 1991;Oliver and Palou 2000;RodriguezBernaldo De Quiros and Costa 2006). The chromatographic methods with the electrochemical detection were also described (Ferruzzi et al 1998;Murata et al 1992).…”

Section: Introductionmentioning

confidence: 99%

Assay of β-Carotene in Dietary Supplements and Fruit Juices by TLC-Densitometry

et al. 2014

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β-carotene as a dietary supplement is a matter of interest to anyone who seeks to improve their skin condition or sight. Due to its antioxidative properties, carotenoids are involved in the prevention of severe conditions, such as cancer, heart disease, macular degeneration, or cataract, which makes them an object of scientific study. Thanks to its color, ß-carotene is used as a food colorant, improving the appearance of foodstuff. A presented method was established for the identification and quantification of β-carotene by thin-layer chromatography with densitometric detection. As a stationary phase, TLC Aluminiumoxid 60 F 254 neutral was utilized. It were activated with methanol and dried at the temperature of 60°C for 1 h. The development was carried out in a twin trough glass chamber saturated with a mobile phase consisting of chloroform/methanol/acetone/ammonium hydroxide (10:22:53:0.2, v/v/v/v). TLC scanner was used for densitometric scanning and analysis in the absorbance mode at 450 nm. The method was validated for specificity, linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ). Presented method illustrates simple, economical, and suitable manner for routine quantitative and qualitative determination of β-carotene in dietary supplements and carrot juice.

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Section: Introductionmentioning

confidence: 99%

Assay of β-Carotene in Dietary Supplements and Fruit Juices by TLC-Densitometry

et al. 2014

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“…Carotenoid concentration was obtained by the respective calibration curve, with β-carotene as pattern substance. Data was obtained by the Software SkanIt 2.4.2 RE for Multiskan Spectrum (Biswas et al, 2011). The results were expressed as mg of β-carotene equivalent/100 g dry weight.…”

Section: Carotenoids Determinationmentioning

confidence: 99%

Mangiferin content, carotenoids, tannins and oxygen radical absorbance capacity (ORAC) values of six mango (Mangifera indica) cultivars from the Colombian Caribbean

Morales¹,

Zapata²,

Jaimes³

et al. 2017

J. Med. Plants Res.

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Mango is one of the tropical fruits of greater production and consumption in the world, and a rich source of bioactive compounds, with various functional properties such as antioxidant activity. In Colombia, mango's market is very broad and diverse. However, there are very few studies that determined the content of bioactive secondary metabolites. The objective of this study was to evaluated the content of different metabolites like Mangiferin, carotenoids, tannins, and the antioxidant capacity by oxygen radical absorbance capacity (ORAC) methodology of six cultivars from the Colombian Caribbean region, with total carotenoid values ranging from 24.67 to 196.15 mg of β-carotene/100 g dry pulp; 84.30 to 161.49 mg Catequine eq./100 g dry pulp for the content of condensed tannins, and 91.80 to 259.23 mg/100 g dry pulp for mangiferin content. The ORAC methodology showed important antioxidant activity results, such as the Chancleta variety with the highest value (2163.78 μmol Trolox/100 g dry pulp). In conclusion, the evaluated mango varieties had promising results as functional food of high nutraceutical value, being Chancleta, Criollo and Jobo varieties, the fruits with highest content in bioactive compounds that expressed the best antioxidant activity.

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“…Minerals of Na, K, Fe, Zn, Cu and P were analyzed by using atomic absorption spectrophotometer [8]. Total phenolic compound was analyzed using Biswas et al [11]. The AOAC method was used to determine the oxalate, tannin and phytate contents of feed sample.…”

Section: Laboratory Analysismentioning

confidence: 99%

Nutrient Composition and Digestibility of Taro Leaf in the Diets of Chicken and Effects on the Meat Quality

Melese¹

2017

JNHFE

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Introduction: the high cost of cereals and protein supplements and uncertainty about their sustainable supply for poultry pressed the need to search for other potential non-conventional feed sources which are relatively less used for human consumption. For stopping this problem the inclusion of taro leaf meals in poultry ration may supply high nutrients for the consumers because taro is multipurpose crop for food and feed sources. Taro leaf is rich in proteins and can be of supplementary protein, carotene and trace minerals in chicken diets. To achieve maximum efficiency in meat quality and production, properly balanced ration is a pre-requisite and taro could be possible alternative feed ingredients in this study.Objective: This study was conducted to evaluate the feeding value of taro leaf in broiler rations which was conducted at Debre Zeit Agricultural Research Center. Method and analysis:The study was carried out using four treatments in which the treatments were inclusion of TL at 0% (T1), 3% (T2), 5% (T3) and 7% (T4). The analysis undertaken in this study was feed DM, Crude Fiber (CF), Total Ash (TA), Ether Extract (EE), Crude Protein (CP) and functional properties (WHC, WA, WSI and WAI) of feed offered. The nutritional composition of chicken meat and cost benefit analysis were also evaluated to see the overall benefit of feeding taro. A total of eighty day-old Cobb500 broiler chicks were used for the study and lasted 56 days. At the end of the experiment, four birds were randomly taken from each pen and slaughtered for nutrient composition and protein quality analysis. Results:The result obtained from the study shows that variability was found as taro leaf level increase and from cost and benefit analyses of the study, taro leaf meal is cost effective meal when used as replacement of expensive and conventional meal in broilers rations Conclusion: Therefore, the present study encourage using taro leaf meal as a good source of nutrients for broilers like other tropical leaves

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A simple UV-Vis spectrophotometric method for determination of β-carotene content in raw carrot, sweet potato and supplemented chicken meat nuggets

Cited by 136 publications

References 12 publications

Assay of β-Carotene in Dietary Supplements and Fruit Juices by TLC-Densitometry

Assay of β-Carotene in Dietary Supplements and Fruit Juices by TLC-Densitometry

Mangiferin content, carotenoids, tannins and oxygen radical absorbance capacity (ORAC) values of six mango (Mangifera indica) cultivars from the Colombian Caribbean

Nutrient Composition and Digestibility of Taro Leaf in the Diets of Chicken and Effects on the Meat Quality

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