“…ELISA has many subtle variations (e.g., direct, indirect, and sandwich ELISA), each of which offers distinct advantages and disadvantages under certain circumstances. Currently, the indirect ELISA and dot blot when used with specific antipest monoclonal antibodies (MAb) are the most common immunoassays used for examining predator gut content (Stuart and Greenstone, 1990;Hagler et al, 1991Hagler et al, , 1992Hagler et al, , 1995Hagler and Naranjo, 1994a,b;Greenstone and Hunt, 1993;Symondson and Liddell, 1993a,b). While each of the immunoassay formats described above has distinct advantages and disadvantages for examining predator gut content, to date, no experimental data exist that directly compares the efficacy of all these various immunoassays.…”