PCR‐based gut content analysis of insect predators: using ribosomal ITS‐1 fragments from prey to estimate predation frequency

Hoogendoorn, Marlijn; Heimpel, George E.

doi:10.1046/j.1365-294x.2001.01316.x

Cited by 185 publications

(202 citation statements)

References 41 publications

(67 reference statements)

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“…There have also been a few studies using target sequences within multi-copy nuclear ribosomal genes. For example Hoogendoorn and Heimpel (2001) investigated predation of the European corn borer Ostrinia nubilalis (Hu¨bner) (Lepidoptera: Crambidae) by Coleomegilla maculata (DeGeer) (Coleoptera: Coccinellidae) and H. axyridis through targeting a section of the ITS 1 region. Other researchers have rejected the ITS 1 region as it can show high levels of intraspecific variation.…”

Section: Principles Of a Dna Based Igp Studymentioning

confidence: 99%

“…For example, Hoogendoorn and Heimpel (2001) investigated detectability of four different sized fragments (492, 369, 256 and 150 bp) of the nuclear ribosomal ITS 1 sequence of O. nubilalis within the gut of C. maculata. The shortest (150 bp) fragment was present for the full 12 h of the feeding assay, whereas the two longest fragments disappeared after 4 h and 5 h respectively.…”

Section: Principles Of a Dna Based Igp Studymentioning

confidence: 99%

“…Hoogendoorn and Heimpel 2001;Juen and Traugott 2005;Weber and Lundgren 2009b). It is, however, possible to make some generalisations about detection periods.…”

Section: Detection Period Of Prey After a Predation Eventmentioning

confidence: 99%

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Detecting arthropod intraguild predation in the field

et al. 2011

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The process of biological control carries a distinct risk that an alien biological control agent (BCA) will become established as an invasive alien species with an associated threat to the local ecosystem biodiversity. It is imperative that a wide-ranging environmental risk assessment (ERA) is performed before the release of any BCA. This should include considering various potential but difficult to observe ecological interactions between the BCA and members of the native community, including disruption of intraguild relationships. Detection of intraguild predation (IGP) events involving predatory arthropods in the field can be done by analyzing their gut contents. Polymerase chain reaction (PCR) is a sensitive and specific tool to identify target prey DNA within a predator's gut. This paper reviews the efficiency of a DNA based approach for detecting IGP in the field, compared with detection by the use of monoclonal antibodies or gas chromatography. Prey specificity, detection times after prey consumption, capacity for quantification, multiple prey targeting and the time and costs involved in developing and using the different methods are considered.

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Section: Principles Of a Dna Based Igp Studymentioning

confidence: 99%

Section: Principles Of a Dna Based Igp Studymentioning

confidence: 99%

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Detecting arthropod intraguild predation in the field

et al. 2011

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“…Recently, gut content analysis using molecular markers have been developed and used for many predatory species (e.g., Agustí et al, 2003;Greenstone, 2003;Hoogendoorn and Heimpel, 2001;Itou et al, 2013;Kamikawa et al, 2016;Symondson, 2002;Wari et al, 2014;Zhang et al, 2007). Phytoseiid mites─especially specialist species─tend to consume spider-mite eggs (Blackwood et al, 2001;Furuichi et al, 2005), and the small size of this preferred prey item hampers detection in the gut.…”

Section: Introductionmentioning

confidence: 99%

Gut-content analysis of predatory phytoseiid mites using fluorescent-labeled polymerase chain reaction: age of spider-mite eggs influences detection rates

Hinomoto

2017

J. Acarol. Soc. Jpn.

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As spider mites become increasing resistant to chemical acaricides, interest in establishing biological control programs using natural enemies has also risen, requiring verification of spidermite predators. Although some phytoseiid mites are considered effective predators in agroecosystems, their small size makes it difficult to confirm predation through field observation. Polymerase chain reaction (PCR) can be an effective detection technique and has been used commonly in studies on microfaunal predator-prey interactions. However, preliminary data revealed that ordinary agarose gel electrophoresis cannot detect PCR products from phytoseiid mites that consumed spider-mite eggs. In this study, I used fluorescent-labeled primers and genetic analyzers to successfully amplify the gut contents of phytoseiid mites and confirmed they were derived from spider-mite eggs based on fragment analysis. The results indicated that spidermite eggs can be detected ≥24 h post-oviposition, but not within 3 h. Thus, fluorescent PCR is an effective tool for elucidating predator-prey interactions among microfaunal food chains.

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“…Molecular DNA makers have enjoyed wide use for diagnostic purposes within and between species in insects Paskewitz 1996, Hoogendoorn andHeimpel 2001). Polymerase chain reaction (PCR)-based analysis allows assays of small amounts of DNA from individual insects as well as permitting high throughput of large numbers of samples.…”

mentioning

confidence: 99%