Variation in the Efficacy of Several Predator Gut Content Immunoassays

Hagler, James R.

doi:10.1006/bcon.1998.0612

Cited by 38 publications

(25 citation statements)

References 28 publications

(39 reference statements)

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“…In addition, large amounts of nontarget insect proteins are released during the grinding process, which reduces the sensitivity of the indirect ELISA (V.P.J., unpublished data; Hagler 1998). However, this shortcoming can be overcome by diluting the sample with buffer before it is added to the well (V.P.J., unpublished data) or in some cases by converting the indirect ELISA to a sandwich ELISA (Hagler 1998).…”

Section: Discussionmentioning

confidence: 99%

An Inexpensive Immunomarking Technique for Studying Movement Patterns of Naturally Occurring Insect Populations

et al. 2006

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Section: Discussionmentioning

confidence: 99%

An Inexpensive Immunomarking Technique for Studying Movement Patterns of Naturally Occurring Insect Populations

et al. 2006

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“…The data presented here and elsewhere (Hagler 1998;Greenstone and Shufran 2003;Chen et al 2000) suggest that there is a huge discrepancy in the sensitivity of gut content assays. Our aim was not to endorse one technique over the other, but rather to provide an assessment of the pros and cons of each procedure.…”

Section: Predator Feeding Trialsmentioning

confidence: 54%

“…Clearly, more rigorous studies are needed to compare the eYcacy of the two types of gut content assay formats among various predator species (Hagler 1998).…”

Section: Predator Feeding Trialsmentioning

confidence: 99%

Identifying the predator complex of Homalodisca vitripennis (Hemiptera: Cicadellidae): a comparative study of the efficacy of an ELISA and PCR gut content assay

et al. 2008

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A growing number of ecologists are using molecular gut content assays to qualitatively measure predation. The two most popular gut content assays are immunoassays employing pest-specific monoclonal antibodies (mAb) and polymerase chain reaction (PCR) assays employing pest-specific DNA. Here, we present results from the first study to simultaneously use both methods to identify predators of the glassy winged sharpshooter (GWSS), Homalodisca vitripennis (Germar) (Hemiptera: Cicadellidae). A total of 1,229 arthropod predators, representing 30 taxa, were collected from urban landscapes in central California and assayed first by means of enzyme-linked immunosorbent assay (ELISA) using a GWSS egg-specific mAb and then by PCR using a GWSS-specific DNA marker that amplifies a 197-base pair fragment of its cytochrome oxidase gene (subunit I). The gut content analyses revealed that GWSS remains were present in 15.5% of the predators examined, with 18% of the spiders and 11% of the insect predators testing positive. Common spider predators included members of the Salticidae, Clubionidae, Anyphaenidae, Miturgidae, and Corinnidae families. Common insect predators included lacewings (Neuroptera: Chrysopidae), praying mantis (Mantodea: Mantidae), ants (Hymenoptera: Formicidae), assassin bugs (Hemiptera: Reduviidae), and damsel bugs (Hemiptera: Nabidae). Comparison of the two assays indicated that they were not equally effective at detecting GWSS remains in predator guts. The advantages of combining the attributes of both types of assays to more precisely assess field predation and the pros and cons of each assay for mass-screening predators are discussed.

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“…Furthermore, is particularly suited to large-scale testing of field samples, such as might be required, for example, in surveys of predation that remains one of the most difficult to study ecological processes but one that is critical to understand if predators are efficient in the agricultural pest control (Naranjo and Hagler 2001). In general, the indirect ELISA is considered a very sensitive method (Hagler 1998) and the S. oleae AS showed to be sufficiently sensitive and accurate to be used in predation studies with field-collected coccinellids. However, the efficiency of indirect ELISA can depend on the predator: prey protein ratio, as shown by .…”

Section: Discussionmentioning

confidence: 99%

Identification of predator–prey relationships between coccinellids and Saissetia oleae (Hemiptera: Coccidae), in olive groves, using an enzyme-linked immunosorbent assay

et al. 2008

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A polyclonal antiserum (AS) was developed and characterized for the detection of immature stages of the black-scale, Saissetia oleae, in whole body homogenized field-collected coccinellid species, using an indirect enzyme-linked immunosorbent assay (ELISA). The indirect ELISA showed to be sensitive to the S. oleae AS, detecting a protein content between 0.118 and 0.0374 lg mL -1 . The specificity of the ELISA was tested by assaying a range of sympatric predators and alternative preys with the S. oleae AS. Coccinellid larvae obtained the highest cross-reaction and a positive-negative threshold was established at 0.674 lg mL -1 protein. A total of 1,322 coccinellids were field-collected in three olive groves located in Trás-osMontes (northeast of Portugal) by the beating technique and were analyzed to detect S. oleae proteins in their guts. Fieldcollected coccinellids which attained a S. oleae protein concentration equivalent higher than the threshold were considered as a positive reaction. In the overall collected coccinellids, 21.2% reacted positively with the S. oleae AS. Chilocorus bipustulatus and coccinellid larvae obtained the highest percentages of positives with 43.4 and 40.8%, respectively. The greatest frequency of positive responses occurred at the beginning of July, mid-August, and midOctober coinciding with the occurrence of the first, second and third instar nymphs of S. oleae, respectively. Thus, in this study, the role of coccinellids as natural control agents of S. oleae was highlighted by the number of individuals and species that tested positive for S. oleae AS.

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Variation in the Efficacy of Several Predator Gut Content Immunoassays

Cited by 38 publications

References 28 publications

An Inexpensive Immunomarking Technique for Studying Movement Patterns of Naturally Occurring Insect Populations

An Inexpensive Immunomarking Technique for Studying Movement Patterns of Naturally Occurring Insect Populations

Identifying the predator complex of Homalodisca vitripennis (Hemiptera: Cicadellidae): a comparative study of the efficacy of an ELISA and PCR gut content assay

Identification of predator–prey relationships between coccinellids and Saissetia oleae (Hemiptera: Coccidae), in olive groves, using an enzyme-linked immunosorbent assay

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