A simple method for the large-scale preparation of mitochondria from microorganisms

Lang, B. Franz; Burger, Gertraud; Doxiadis, Ilias; Thomas, David Y.; Bandlow, Wolfhard; Kaudewitz, F.

doi:10.1016/0003-2697(77)90295-0

Cited by 119 publications

(23 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sporangia were grown on PmTG (Barr 1986) nutrient agar. Cells were broken with glass beads (Lang et al 1977) and DNA was isolated as described in Cermakian et al (1996). PCR experiments and subsequent cloning and sequencing procedures were also performed as described in Cermakian et al (1996), using the PCR primers R-8.1 and R-3.2.…”

Section: Methodsmentioning

confidence: 99%

On the Evolution of the Single-Subunit RNA Polymerases

et al. 1997

View full text Add to dashboard Cite

Abstract. Many eukaryotic nuclear genomes as well as mitochondrial plasmids contain genes displaying evident sequence similarity to those encoding the singlesubunit RNA polymerase (ssRNAP) of bacteriophage T7 and its relatives. We have collected and aligned these ssRNAP sequences and have constructed unrooted phylogenetic trees that demonstrate the separation of ssRNAPs into three well-defined and nonoverlapping clusters (phage-encoded, nucleus-encoded, and plasmidencoded). Our analyses indicate that these three subfamiles of T7-like RNAPs shared a common ancestor; however, the order in which the groups diverged cannot be inferred from available data. On the basis of structural similarities and mutational data, we suggest that the ancestral ssRNAP gene may have arisen via duplication and divergence of a DNA polymerase or reverse transcriptase gene. Considering the current phylogenetic distribution of ssRNAP sequences, we further suggest that the origin of the ancestral ssRNAP gene closely paralleled in time the introduction of mitochondria into eukaryotic cells through a eubacterial endosymbiosis.

show abstract

Section: Methodsmentioning

confidence: 99%

On the Evolution of the Single-Subunit RNA Polymerases

et al. 1997

View full text Add to dashboard Cite

show abstract

“…Preparation of yeast mitochondria was done according to a modified method by Lang et al [20]. All steps were performed in cold.…”

Section: Preparation Of Mitochondria Submitochondrial Particles and mentioning

confidence: 99%

Cross‐reconstitution of isolated F₁‐ATPase from potato tuber mitochondria with F₁‐depleted beef heart and yeast submitochondrial particles

1987

View full text Add to dashboard Cite

Cross-reconstitution of isolated potato mitochondrial Fr-ATPase with Fr-depleted beef heart and yeast submitochondrial particles is reported. Potato Fr binds to the heterologous membrane and confers oligomycin sensitivity on the ATPase activity of the reconstituted system. Binding of F1 is promoted by the presence of Mg2+ with the maximal stimulatory effect at 20 mM. Mgz+ increase the sensitivity to oligomycin of the reconstituted system consisting of potato F1 and yeast membranes, however, they do not influence oligomytin sensitivity of potato Fr and heef heart membranes.

show abstract

“…All further procedures were carried out at 2 "C. Cells were washed twice with water and twice with homogenization medium (8.5 ' %; mannitol, 30 m M Hepes/KOH, pH 7.4, 100 mM potassium acetate, 2 mM magnesium acetate, 2 mM dithiothreitol). They were broken essentially as described by Lang et al [7]. Up to 5 g cells (wet weight) were suspended in homogenization medium (2.5 ml/g wet weight) together with glass beads (0.45-0.5 mm diameter, 3-4 g/ml suspension) in a 35-ml centrifuge beaker.…”

Section: Growth Of Strainsmentioning

confidence: 99%

Preparation of a mRNA‐Dependent Cell‐Free Translation System from Whole Cells of Saccharomyces cerevisiae

Hofbauer

Fessl

Hamilton

et al. 1982

European Journal of Biochemistry

View full text Add to dashboard Cite

A cell‐free protein‐synthesizing system has been prepared from Saccharomyces cerevisiae by mechanical breakage of cells, isolation of a 30000xg supernatant fraction and removal of endogenous mRNA by treatment with micrococcal nuclease. The system thus isolated is dependent on added mRNA and translates yeast mRNA to discrete products, many of them identical with yeast proteins synthesized in vivo. Activity and properties of this system are comparable to those of other eukaryotic cell‐free translation systems. It offers the following advantages compared to yeast translation systems described previously. (a) Its isolation is simple and fast. (b) Since it is not isolated from spheroplasts there is no danger of its inactivation by contaminants in enzymes used for spheroplast preparation. (c) Isolation appears to be less strain‐dependent and can be carried out starting from cells in various physiological states.

show abstract

A simple method for the large-scale preparation of mitochondria from microorganisms

Cited by 119 publications

References 8 publications

On the Evolution of the Single-Subunit RNA Polymerases

On the Evolution of the Single-Subunit RNA Polymerases

Cross‐reconstitution of isolated F₁‐ATPase from potato tuber mitochondria with F₁‐depleted beef heart and yeast submitochondrial particles

Preparation of a mRNA‐Dependent Cell‐Free Translation System from Whole Cells of Saccharomyces cerevisiae

Contact Info

Product

Resources

About

A simple method for the large-scale preparation of mitochondria from microorganisms

Cited by 119 publications

References 8 publications

On the Evolution of the Single-Subunit RNA Polymerases

On the Evolution of the Single-Subunit RNA Polymerases

Cross‐reconstitution of isolated F1‐ATPase from potato tuber mitochondria with F1‐depleted beef heart and yeast submitochondrial particles

Preparation of a mRNA‐Dependent Cell‐Free Translation System from Whole Cells of Saccharomyces cerevisiae

Contact Info

Product

Resources

About

Cross‐reconstitution of isolated F₁‐ATPase from potato tuber mitochondria with F₁‐depleted beef heart and yeast submitochondrial particles