A simple method for extracting plant phenolic compounds

Sauvesty, A.; Page, Frederick; Huot, Jean

doi:10.1139/x92-087

Cited by 47 publications

(25 citation statements)

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“…To analyze total nonstructural carbohydrates, amino acids, total phenolics, and condensed tannins, samples were extracted with 80% aqueous ethanol. The samples were placed in a sonicator for 30 min and then centrifuged (Sauvesty et al 1992;A. Hagerman personal communication).…”

Section: Methodsmentioning

confidence: 99%

The effect of elevated CO 2 and N availability on tissue concentrations and whole plant pools of carbon-based secondary compounds in loblolly pine ( Pinus taeda )

1997

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We examined the extent to which carbon investment into secondary compounds in loblolly pine (Pinus taeda L.) is changed by the interactive effect of elevated CO and N availability and whether differences among treatments are the result of size-dependent changes. Seedlings were grown for 138 days at two CO partial pressures (35 and 70 Pa CO) and four N solution concentrations (0.5, 1.5, 3.5, and 6.5 mmol l NONH) and concentrations of total phenolics and condensed tannins were determined four times during plant development in primary and fascicular needles, stems and lateral and tap roots. Concentrations of total phenolics in lateral roots and condensed tannins in tap roots were relatively high regardless of treatment. In the smallest seedlings secondary compound concentrations were relatively high and decreased in the initial growth phase. Thereafter condensed tannins accumulated strongly during plant maturation in all plant parts except in lateral roots, where concentrations did not change. Concentrations of total phenolics continued to decrease in lateral roots while they remained constant in all other plant parts. At the final harvest plants grown at elevated CO or low N availability showed increased concentrations of condensed tannins in aboveground parts. The CO effect, however, disappeared when size differences were adjusted for, indicating that CO only indirectly affected concentrations of condensed tannins through accelerating growth. Concentrations of total phenolics increased directly in response to low N availability and elevated CO in primary and fascicular needles and in lateral roots, which is consistent with predictions of the carbon-nutrient balance (CNB) hypothesis. The CNB hypothesis is also supported by the strong positive correlations between soluble sugar and total phenolics and between starch and condensed tannins. The results suggest that predictions of the CNB hypothesis could be improved if developmentally induced changes of secondary compounds were included.

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Section: Methodsmentioning

confidence: 99%

The effect of elevated CO 2 and N availability on tissue concentrations and whole plant pools of carbon-based secondary compounds in loblolly pine ( Pinus taeda )

1997

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“…Phenolic compounds were extracted according to the method outlined by Sauvesty et al (1992). A known weight of the dry powdered tissues was extracted three times with 70% ethanol at 40°C for 4 h. Each extract was centrifuged for 15 min at 3000g.…”

Section: Extraction and Determination Of Phenolicsmentioning

confidence: 99%

Phytotoxicity of Euphorbia helioscopia L. on Triticum aestivum L. and Pisum sativum L.

Madany

Saleh

2015

Annals of Agricultural Sciences

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Invasive field weeds, such as Euphorbia sp., represent main threat for crop productivity. The present work was conducted to evaluate the phytotoxicity of Euphorbia helioscopia on wheat (Triticum aestivum L.) and pea (Pisum sativum L.). The influence of aqueous extract from shoot of E. helioscopia, at different rates (1.0%, 2.5%, 5.0%, and 10.0%; w/v), on germination and early seedling growth of wheat and pea as well as some of the synchronized physiological aspects was investigated. E. helioscopia aqueous extract severely affected the germination in a concentration dependent manner. Plumule and radicle length, as well as their fresh and dry masses were markedly reduced. Moreover, amylase activity and total soluble sugars were significantly reduced in response to treatment with aqueous Euphorbia extract, in both test plants, whereas, proteolytic activity showed marked improvement. The linear regression analysis revealed the presence of positive linear correlation between germination rate and amylase activity. The stress markers such as proline, phenolics and flavonoids were markedly accumulated upon treatment with E. helioscopia extract. The increment in the level of total phenolics was concomitant with an improvement in phenylalanine ammonia lyase and peroxidase activities. High performance liquid chromatography analysis of E. helioscopia extract revealed the presence of two cinnamic acid derivatives (caffeic and p-coumaric acids), two benzoic acid derivatives (vanillic and syringic acids) and a flavanonol (Dihydroquercetin). To our knowledge, this is the first study to investigate the physiological implications underlying the phytotoxicity of E. helioscopia.ª 2015 Production and hosting by Elsevier B.V. on behalf of Faculty of Agriculture, Ain Shams University.

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“…Além disso, microrganismos proteolíticos são de ocorrência generalizada em solos e biossólidos (HANKIN e HILL, 1978;LOLL e BOLLAG, 1983), confirmando a importância das proteínas como substrato para a atividade microbiana. No caso de fenóis e taninos, normalmente solúveis em água, estes podem formar complexos altamente resistentes com proteínas durante a decomposição de resíduos, resultando em redução das taxas de mineralização do C e do N (SAUVESTY et al, 1992).…”

Section: Relação Entre Degradação E Composição Química Da Mo Dos Biosunclassified

Cinética de degradação da matéria orgânica de biossólidos após aplicação no solo e relação com a composição química inicial

Andrade

Oliveira²,

Cerri³

2006

Bragantia

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O objetivo deste estudo foi avaliar a degradação de biossólidos após aplicação no solo, relacionando com a composição química inicial da matéria orgânica (MO) desses resíduos. Foram utilizados quatro biossólidos e um composto orgânico à base de lodo de esgoto, provenientes de diferentes sistemas de tratamento de esgotos e/ou estabilização do lodo e/ou condicionamento químico para desidratação e/ou etapa complementar visando à melhor adequação ao uso agrícola. A degradação dos biossólidos foi determinada com quantificação do CO2 emanado a partir de experimento de incubação de misturas de amostras de um Latossolo, com dose dos resíduos correspondente a 40 t ha-1. As taxas de degradação da fração orgânica dos resíduos variaram entre 5% e 22%. De modo geral, a degradação da fração orgânica dos biossólidos foi descrita por equação de cinética química com duas fases: a primeira fase caracterizou-se pela elevada velocidade de degradação de compostos orgânicos presentes em quantidades limitadas, cuja exaustão do substrato ocorreu em poucos dias (2 a 20 dias); a segunda fase caracterizouse pela redução da velocidade da reação de degradação e aumento da quantidade de carbono mineralizado (65% do total de C mineralizado no período). A proteína bruta, expressa como porcentagem do conteúdo orgânico dos resíduos, foi o parâmetro que melhor correlacionou com a taxa de degradação dos biossólidos no fim de 70 dias de incubação (r = 0,999 e Prob. > t inferior a 10-4), sendo promissora sua utilização na previsão da taxa de degradação da MO de biossólidos após aplicação no solo. A participação do compartimento protéico foi crescente com o tempo de incubação, comprovando que no início do período de avaliação outros compostos orgânicos mais lábeis funcionaram como fonte de carbono e de energia para a microbiota edáfica.

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A simple method for extracting plant phenolic compounds

Cited by 47 publications

References 0 publications

The effect of elevated CO 2 and N availability on tissue concentrations and whole plant pools of carbon-based secondary compounds in loblolly pine ( Pinus taeda )

The effect of elevated CO 2 and N availability on tissue concentrations and whole plant pools of carbon-based secondary compounds in loblolly pine ( Pinus taeda )

Phytotoxicity of Euphorbia helioscopia L. on Triticum aestivum L. and Pisum sativum L.

Cinética de degradação da matéria orgânica de biossólidos após aplicação no solo e relação com a composição química inicial

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