A Simple Method for Controlling the Degree of PEGylation of Dynorphin A (1–13)

Abstract

Dynorphin A (1-13) was chemically modified by reaction with succinimidyl propionate-monomethoxy polyethylene glycol (mPEG-SPA). To determine the degree and the optimized condition for PEGylation of dynorphin A, the reactions were monitored in different pH buffers at different molar ratios by reversed-phase high performance liquid chromatography (RP-HPLC) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The results showed that the degree of PEGylation for dynorphin A could easily be controlled through adjustment of the molar ratios and the pH. The degree of PEGylation of dynorphin A increased as the molar ratio of dynorphin to MPEG-SPA and the pH increased.

Stability of Co–Ce–Mn mixed-oxide catalysts for CO preferential oxidation in H2-rich gases

“…The area of the low-temperature peaks is much greater for the pre-reduced sample than for the fresh catalyst, indicating that the reduced catalyst can adsorb much more CO 2 than the fresh sample. This may be attributed to that the reduced sample can offer electrons to CO 2 , which favors CO 2 adsorption [30][31][32].…”

Adsorption and Release Mechanism of Polypeptide on Magnetic Mesoporous Carbons

“…Dynorphin A (1-13) (C 75 H 126 N 24 O 15 , mw/1604) 13 , which had a bio-polypeptide containing 13 amino acids, had analgesic effect and played a significant role in regulating the cardiovascular and respiratory systems. Drug loading on the support Magnetic Fe-containing mesoporous carbons with high surface areas and pore volume were synthesized through a simple soft-template route, wherein phenolic resin was used as a carbon precursor, triblock copolymer F127 as a template agent, tetraethyl orthosilicate as a silica precursor and hydrated iron nitrite as an iron source.…”