New versatile approach for analysis of PEG content in conjugates and complexes with biomacromolecules based on FTIR spectroscopy.

Le-Deygen, Irina M.; Kudryashova, Еlena V.

doi:10.1016/j.colsurfb.2016.01.030

Cited by 82 publications

(41 citation statements)

References 28 publications

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“…Changes in the lipid packing density and physical state (e.g., gel or liquid-crystalline) can be detected using characteristic frequency (wavenumber) bands of absorption of lipid methylene, carbonyl, and phosphate groups. High sensitivity of ATR-FTIR spectroscopy in physicochemical studies of liposome complexes with small molecule drugs [46,47] and PEG-chitosan complex [48] has been demonstrated previously.…”

Section: Atr-ftir Spectroscopymentioning

confidence: 68%

Phosphatidylinositol Stabilizes Fluid-Phase Liposomes Loaded with a Melphalan Lipophilic Prodrug

et al. 2021

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Previously, a liposomal formulation of a chemotherapeutic agent melphalan (Mlph) incorporated in a fluid lipid bilayer of natural phospholipids in the form of dioleoylglyceride ester (MlphDG) was developed and the antitumor effect was confirmed in mouse models. The formulation composed of egg phosphatidylcholine (ePC), soybean phosphatidylinositol (PI), and MlphDG (8:1:1, by mol) showed stability in human serum for at least 4–5 h. On the contrary, replacing PI with pegylation of the liposomes, promoted fast dissociation of the components from the bilayer. In this work, interactions of MlphDG-liposomes with the most abundant plasma protein—albumin—in function of the presence of PI in the formulation were explored using Fourier transform infrared spectroscopy. The release of MlphDG from the liposomes was studied by asymmetrical flow field-flow fractionation (AF4) using micelles formed by a polyethylene glycol conjugate with phosphatidylethanolamine to mimic the physiological lipid sink like lipoproteins. Our results show that PI actually protects the membrane of MlphDG-liposomes from the protein penetration, presumably due to pairing between the positively charged MlphDG and negatively charged PI, which compensates for the heterogeneity of the lipid bilayer. The AF4 technique also evidences high stability of the formulation as a drug carrier.

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Section: Atr-ftir Spectroscopymentioning

confidence: 68%

Phosphatidylinositol Stabilizes Fluid-Phase Liposomes Loaded with a Melphalan Lipophilic Prodrug

et al. 2021

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“…A small, broad peak at ≈1115 cm −1 observed for GelMA‐PEG is likely due to the COC bond stretching of the PEG portion of that molecule. [ 24 ]…”

Section: Resultsmentioning

confidence: 99%

A Carbodiimide Coupling Approach for PEGylating GelMA and Further Tuning GelMA Composite Properties

Comeau

Willett

2020

Macro Materials & Eng

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To date the low mechanical strength and high swelling rate of gelatin‐based products have limited their use toward many tissue engineering targets. Here a new approach to tuning methacrylated gelatin (GelMA) properties involving the carbodiimide coupling of polyethylene glycol (PEG) to the GelMA molecule is investigated. PEGylation improves the capacity of the solution to flow and reduces hydrogel swelling, while in composite form with nano‐hydroxyapatite (nHA) particles there is no impact on swelling. Differential scanning calorimetry (DSC) reveals that PEGylation likely restores some of the triple helical nature of gelatin to the GelMA and increases steric hindrance. Furthermore, triethyleneglycol dimethacrylate inclusion in GelMA‐PEG composites is able to address the loss in dynamic stiffness reported as a result of PEGylation. Altogether PEGylated GelMA shows evidence of reduced interaction with water compared to control GelMA, whether alone or in composite form. By tuning this interaction and reducing the volume of water necessary to solvate GelMA, the potential to use GelMA composites for a variety of tissue engineering applications is greatly improved.

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“…The peaks around 1100 cm -1 were the characteristic peak for stretching in C-C, C-O, and C=O bonds as well as bending vibration in C-C and C-O bonds (Chylińska et al 2016;Muthukumaran & Philip 2016). The band at 1146 cm -1 in Figure 1(a) was the main characteristic of polyethylene glycol (PEG) molecules (Deygen & Kudryashova 2016), which can be further used for determination either it was related to trans conformation or gauche conformation with respective to C-C bond while the asymmetric PO 2 stretching region can be observed by the peak at 1240 cm -1 (Varga et al 2013). The significant peak around 1700 cm -1 was due to the presence of stretching vibration at the C=O bond in all materials Varga et al 2013).…”

Section: Resultsmentioning

confidence: 99%

Pegylated Oleic Acid-Lecithin Liposomes (Poll) for Anticancer Drug Delivery

Suk¹,

Chung²,

Misran³

2020

JSM

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Cancer is a major health issue, conferring to more than 14.5 million deaths worldwide. Liposomes, self-assembly amphiphilic bilayer molecules, served as excellent alternative vehicles due to their ability to encapsulate both hydrophobic and hydrophilic anticancer drugs. Conventional liposomes, comprised mainly phospholipids are costineffective, unstable, and easily degraded by the external environment. In this study, we introduced PEGylated oleic acid-lecithin liposomes constructed by using C-18 monounsaturated fatty acids (oleic acid) and soy lecithin, in the presence of DOPEPEG2000 in pH7.4, above their glass transition temperature, T g , by employing the simple thin layer lipid hydration method. FTIR spectrum of oleic acid, soy lecithin, and DOPEPEG2000 was studied. The average particle size without further mechanical interference was 1102.3 nm while the zeta potential value was -18 mV, which is compatible with the zeta potential of the red blood cell. The polydispersity index (PDI) was reduced by 46.2% with the incorporation of the DOPEPEG2000. The morphological study using OPM showed the presence of spherical shape liposomes that exhibit the birefringence effect under the light field and Maltese cross under the dark field. Encapsulation of folinic acid, methotrexate, doxorubicin, or irinotecan resulted in greater than 75% encapsulation efficiency (EE). Half-maximal inhibitory concentration, IC 50 , was significantly reduced in POLL as compared to free anticancer drugs. Our data demonstrate POLL may be a promising alternative vehicle to deliver various anticancer drugs to targeted tumour sites. ABSTRAKBarah merupakan isu kesihatan utama yang menyebabkan lebih 14.5 juta kematian di seluruh dunia. Liposom, iaitu molekul swabentuk dwilapis merupakan penghantar alternatif terbaik berikutan kebolehannya untuk mengkapsulkan ubatan anti barah hidrofobik dan hidrofilik. Liposom yang lazimnya diperbuat daripada fosfolipid adalah tidak efektif daripada segi kos, tidak stabil dan mudah terurai oleh persekitaran luar. Dalam kajian ini, kami memperkenalkan liposom asid olik-lesitin berPEG yang terdiri daripada asid lemak tak tepu C-18 (asid olik) dan lesitin soya, dengan kehadiran DOPEPEG2000 dalam pH7.4, pada suhu lebih tinggi daripada suhu peralihan gelas T g , menggunakan metod hidrasi lapisan nipis lipid. Spektrum FTIR asid olik, lesitin soya, dan DOPEPEG2000 juga turut dikaji. Purata saiz partikel adalah 1102.3 nm manakala keupayaan zeta adalah -18 mV, sesuai dengan keupayaan zeta sel darah merah. Indeks polisebaran telah berkurang sebanyak 46.2% dengan penambahan DOPEPEG2000. Kajian morfologi menggunakan Mikroskop Optikal Pengutuban menunjukkan kewujudan liposom berbentuk sfera yang menghasilkan kesan dwibiasan pada medan cerah dan silang Maltese pada medan gelap. Pengkapsulan asid folinik, metotreksat, doksorubisin atau irinotecan menghasilkan lebih 75% kecekapan pengkapsulan. Kepekatan separa rencatan, IC 50 , telah berkurang dengan signifikan pada POLL berbanding ubatan anti barah. Data menunjukkan POLL mungkin ...

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New versatile approach for analysis of PEG content in conjugates and complexes with biomacromolecules based on FTIR spectroscopy.

Cited by 82 publications

References 28 publications

Phosphatidylinositol Stabilizes Fluid-Phase Liposomes Loaded with a Melphalan Lipophilic Prodrug

Phosphatidylinositol Stabilizes Fluid-Phase Liposomes Loaded with a Melphalan Lipophilic Prodrug

A Carbodiimide Coupling Approach for PEGylating GelMA and Further Tuning GelMA Composite Properties

Pegylated Oleic Acid-Lecithin Liposomes (Poll) for Anticancer Drug Delivery

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