A simple graphical representation of selectivity in hydrophilic interaction liquid chromatography

Hydrophilic-subtraction model for the characterization and comparison of hydrophilic interaction liquid chromatography columns

“…Based on the magnitude of c values, the capacity of cationexchange for these stationary phases increases: Polysulfoethyl A < BEH HILIC < XCharge WCX < Unitary Silica < Atlantis HILIC Silica. Although PolySulfoethyl A has negative terminal sulfonates, it does not display significant cation-exchange property, consistent with the results reported by Dinh et al [24] and Mohammed et al [47]. This can be explained that the residual aminopropyl groups are protonated in our experiments, which renders PolySulfoethyl A with zwitterionic properties.…”

Hybrid carbon nanoparticles modified core–shell silica: A high efficiency carbon-based phase for hydrophilic interaction liquid chromatography

“…The presence of this water layer has recently been confirmed by Dinh et al using Karl Fischer titrations [13]. In addition to partitioning, other interactions have been shown to be involved in HILIC retention, including H-bonding [14,15], ionexchange [16,17], adsorption [18] and dipole-dipole interactions [17].…”

“…This is consistent with the selectivity observed for a silica monolith coated with the same type of latex (column no. 25) [15]. Coating the latexed core-shell silica with the carboxylate modified carbon nanoparticles results in an increase of the k cytosine /k uracil ratio from 2.9 to 6.9 (i.e.…”

“…On the other hand, the ratio k cytosine /k uracil has been suggested as a measure of the column hydrophilicity based on their difference in octanol/water partition coefficients [4,17]. Using retention ratios rather than absolute retentions have the advantage of cancelling out other parameters affecting retention such as surface area and pore size [15].…”

“…S4) for the effect of pH on the retention of benzoic acid on the hybrid HILIC, the AS9-SC latex coated and the bare core-shell silica phases. simple and convenient selectivity plot to classify different HILIC phases [15]. The HILIC selectivity plot (Fig.…”