A Simple and Reproducible Method of Isolating Rat Peritoneal Mast Cells in High Yield and Purity

Ph, Cooper; Dr, Stanworth

doi:10.1080/00327487408068766

Cited by 12 publications

(5 citation statements)

References 6 publications

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“…The resulting suspensions contained 80-90 % mast cells as judged microscopically by toluidine blue staining (Cooper and Stanworth, 1974) and radioactive uptake (Langunoff et al, 1960). Viability was determined by the trypan blue dye exclusion test (Phillips, 1973).…”

Section: Mast-cell Isolation and Implantationmentioning

confidence: 99%

Mast cells and tumor angiogenesis

Kessler

Langer

Pless

et al. 1976

Intl Journal of Cancer

247

113

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Tumor angiogenesis factor (TAF) elicits a strong vasoproliferative response when implanted upon the chorioallantoic membrane (CAM) of the chick embryo. This response is first observed stereomicroscopically 2-3 days after implantation. A 40-fold increase in mast cell density is observed within the vicinity of this implant by 24 h. Mast cells that have been isolated from retired breeder Sprague-Dawley rats fail to evoke a vascular reaction when implanted on the CAM. An intermediate role for the mast cell in tumor angiogenesis is suggested.

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Section: Mast-cell Isolation and Implantationmentioning

confidence: 99%

Mast cells and tumor angiogenesis

Kessler

Langer

Pless

et al. 1976

Intl Journal of Cancer

247

113

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“…Mast cells were isolated over bovine serum albumin (BSA, 38% w/v; Sigma) [27], ficoll (30% w/v: Sigma) [30], metrizamide (22.5% w/v; Sigma) [28] or Percoll (density 1.11 g/m l; Pharmacia) [29]. In each case the well-mixed peritoneal cell suspension was carefully layered above the medium, allowing it to flow against the side of a polystyrene tube held in an inclined position.…”

Section: Purification O F Peritoneal Rat Mast Cellsmentioning

confidence: 99%

Contribution of Post-Secretory Mechanisms to the Observed Pattern of Histamine and 5-Hydroxytryptamine Secretion from Peritoneal Rat Mast Cells in Response to Compound 48/80

Purcell¹,

Cohen²,

Hanahoe³

1989

Int Arch Allergy Immunol

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Compound 48/80 induces a greater percentage release of histamine than 5-HT from purified peritoneal rat mast cells such that the log-concentration effect curves diverge. Mast cells were shown to remove histamine and 5-HT applied exogenously, although uptake of 5-HT was in preference to that of histamine. At concentrations of clomipramine and fluoxetine that were shown to inhibit 5-HT uptake, the net percentage release of 5-HT increased in a corresponding manner. Likewise, pargyline and nialamide, which inhibit monoamine oxidase, potentiated the release of 5-HT, although they were without effect upon 5-HT uptake. Observed differences in the pattern of histamine and 5-HT secretion are proposed to be the result of post-secretory events involving amine reuptake and metabolism rather than a reflection of differential release mechanisms.

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“…For some experiments mast cells were separated from other cell types in the peritoneal cavity by the method of Cooper and Stanworth (1974) using a 30 per cent. Ficoll gradient containing 0.25 per cent.…”

Section: Patricia R Mann a N D C N D Cruickshankmentioning

confidence: 99%

An eosinophilotactic factor derived from rat mast cells

Mann¹,

Cruickshank²

1975

The Journal of Pathology

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Rat eosinophils have been showed to respond chemotactically to the supernatant from mast cells which have been disrupted by freezing and thawing and from mast cells which have been specifically degranulated by Compound 48/80 or by antigen challenge or sensitised cells. The fact that the chemotactic response is not dependent on allergic degranulation suggests that the chemotactic factor is present pre-formed in the mast cells rather than being generated in response to an antigen-antibody reaction.

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A Simple and Reproducible Method of Isolating Rat Peritoneal Mast Cells in High Yield and Purity

Cited by 12 publications

References 6 publications

Mast cells and tumor angiogenesis

Mast cells and tumor angiogenesis

Contribution of Post-Secretory Mechanisms to the Observed Pattern of Histamine and 5-Hydroxytryptamine Secretion from Peritoneal Rat Mast Cells in Response to Compound 48/80

An eosinophilotactic factor derived from rat mast cells

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