Contribution of Post-Secretory Mechanisms to the Observed Pattern of Histamine and 5-Hydroxytryptamine Secretion from Peritoneal Rat Mast Cells in Response to Compound 48/80

Abstract

Compound 48/80 induces a greater percentage release of histamine than 5-HT from purified peritoneal rat mast cells such that the log-concentration effect curves diverge. Mast cells were shown to remove histamine and 5-HT applied exogenously, although uptake of 5-HT was in preference to that of histamine. At concentrations of clomipramine and fluoxetine that were shown to inhibit 5-HT uptake, the net percentage release of 5-HT increased in a corresponding manner. Likewise, pargyline and nialamide, which inhibit monoamine oxidase, potentiated the release of 5-HT, although they were without effect upon 5-HT uptake. Observed differences in the pattern of histamine and 5-HT secretion are proposed to be the result of post-secretory events involving amine reuptake and metabolism rather than a reflection of differential release mechanisms.

Changes in histamine and serotonin secretion from rat peritoneal mast cells caused by antidepressants

“…4). This confirms the results of Purcell et al [3,4] which suggests that the increase of serotonin in the medium after the secretion is due to the inhibition of serotonin reuptake into mast cells caused by antidepressants. Our present results further show that the elevation of serotonin in the medium following the application of compound 48/80 caused by antidepressants occurs only in the presence of extracellular Ca 2+ ions (Fig.…”

“…Preincubation of mast cells with some antidepressants (clomipramine and fluoxetine (10 _8-10-Stool/I) before stimulation with compound 48/80 has no effect on the amount of histamine in the incubation medium, but produces a concentration-dependent increase in the content of serotonin in the medium [3]. Since antidepressants are selective inhibitors of serotonin uptake, the increase of serotonin in the medium could be due to an inhibition of serotonin reuptake into mast cells after the secretion [3,4]. However, various psychotropic drugs were also reported to exert an inhibitory effect on the secretion of the amines [5][6][7].…”

Mechanism of airway hyperresponsiveness to adenosine induced by allergen challenge in actively sensitized Brown Norway rats

“…Since the functional correlate is bronchoconstriction it is reasonable to speculate that the tissue location of this sub‐population of mast cells is the lung. Such a scenario might not be unexpected given that mast cells contain both histamine and 5‐HT ( Benditt et al ., 1955 ; Purcell et al ., 1989a , 1989b ; Purcell & Hanahoe 1990a , 1990b ), that there is differential storage of histamine and 5‐HT in different anatomical locations ( Purcell et al ., 1989a ) and that secretion patterns of histamine and 5‐HT vary in mast cells from different locations or in response to different secretagogues ( Purcell et al ., 1989a ; Purcell & Hanahoe, 1990a ). Importantly, the concept receives support from two further experimental observations.…”

Characteristics of the inhibitory effect of tricyclic antidepressants on histamine release from rat peritoneal mast cells

“…In Ca 2 + -containing medium, preincubation of mast cells with amitriptyline inhibits histamine release [1], whereas some other antidepressants (clomipramine, fluoxetine) have no inhibitory effect on the secretion [2,3]. Our previous results show that the inhibitory effect of some tricyclic antidepressants is greater in the absence of extracellular Ca 2 + ions than in the medium containing physiological concentrations of Ca 2 + ions.…”