A Simple and Rapid RP‐HPLC Method for the Estimation of Nevirapine in Bulk and Pharmaceutical Dosage Forms

Abstract

A reverse phase HPLC method is described for the determination of nevirapine in tablet dosage forms. Chromatography was carried on an ODS column using a mixture of methanol and water (89:11 v/v) as the mobile phase at a flow rate of 1 mL/min with detection at 284 nm. The retention time of the drug was 3.22 min. The detector response was linear in the concentration of 25-200 mcg/mL. The limit of detection and limit of quantification was 0.004 and 0.0121 mcg/mL respectively. The percentage assay of nevirapine was 99.52%. The method was validated by determining its sensitivity, accuracy and precision. The proposed method is simple, economical, fast, accurate and precise and hence can be applied for routine quality control of nevirapine in bulk and tablet dosage forms.

Application of MWCNT/Ag‐Pt Nanocomposite Modified GCE for the Detection of Nevirapine in Pharmaceutical Formulation and Biological Samples

“…The most successful analysis technique requires high specificity, sub-nanograms detection limits in biological fluids and robustness. Various reported methods for NVP determination, involves use of reverse phase liquid chromatography (RP-HPLC [Kabra et al, 2009;Mohanraj et al, 2008], capillary electrophoresis , HPLC-UV [Marinho et al, 2014], LC-MS/MS [Zhang et al, 2010] and GC-MS [Vogel et al, 2010]. Some of these methods have low sensitivity, time consuming sample preparation and costly (equipment and analysis).…”