A signal-switchable electrochemiluminescence biosensor based on the integration of spherical nucleic acid and CRISPR/Cas12a for multiplex detection of HIV/HPV DNAs

Zhao, Kai-Ren; Wang, Li; Liu, Pengfei; Hang, Xiao-Min; Wang, Hui-Yi; Ye, Shu-Ying; Liu, Zhijun; Liang, Guoxi

doi:10.1016/j.snb.2021.130485

Cited by 42 publications

(27 citation statements)

References 49 publications

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“…In recent years, CRISPR/Cas9-mediated antiviral protocols to manipulate infectious human viruses have been applied efficiently. In this regard, the CRISPR/Cas9 system has shown remarkable efficacy against human immunodeficiency virus (HIV), hepatitis B virus (HBV), and HPV [ 45 , 46 ].…”

Section: Crispr/cas9 Applications In Viral Infections and Genetic Dis...mentioning

confidence: 99%

“…CRISPR/Cas9 tools have great capacity for the diagnosis and treatment of cancer, including (1) the use of CRISPR/Cas9-based diagnostic systems SHERLOCK and DETECTR for cancer diagnostics, (2) providing TCR knockout (KO) CAR-T cells (universal CAR-T cells), (3) KO of inhibitory receptors such as PD-1 and LAG-3 to promote the capability of cancer immunotherapy, (4) elimination of oncogenic virus-like HPV, (5) and establishment of in vivo tumor models by eliciting mutations in several genes [ 7 , 45 , 95 – 97 ] (Fig. 2 ).…”

Section: Crispr/cas9 In Cancersmentioning

confidence: 99%

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CRISPR/Cas9 application in cancer therapy: a pioneering genome editing tool

et al. 2022

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The progress of genetic engineering in the 1970s brought about a paradigm shift in genome editing technology. The clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9 (CRISPR/Cas9) system is a flexible means to target and modify particular DNA sequences in the genome. Several applications of CRISPR/Cas9 are presently being studied in cancer biology and oncology to provide vigorous site-specific gene editing to enhance its biological and clinical uses. CRISPR's flexibility and ease of use have enabled the prompt achievement of almost any preferred alteration with greater efficiency and lower cost than preceding modalities. Also, CRISPR/Cas9 technology has recently been applied to improve the safety and efficacy of chimeric antigen receptor (CAR)-T cell therapies and defeat tumor cell resistance to conventional treatments such as chemotherapy and radiotherapy. The current review summarizes the application of CRISPR/Cas9 in cancer therapy. We also discuss the present obstacles and contemplate future possibilities in this context.

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Section: Crispr/cas9 Applications In Viral Infections and Genetic Dis...mentioning

confidence: 99%

Section: Crispr/cas9 In Cancersmentioning

confidence: 99%

CRISPR/Cas9 application in cancer therapy: a pioneering genome editing tool

et al. 2022

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Section: Biosensors For the Detection Of Pathogensmentioning

confidence: 99%

“…Electrochemiluminescence (ECL) biosensors for the detection of viruses, namely HIV and human papilloma virus (HPV) have been reported [64] , [65] , [66] . Zhao et al integrated spherical nucleic acid (SNA) with CRISPR/Cas12a for the detection of HIV and HPV [64] . An “on/off” signal switchable biosensor was fabricated such that a sandwich structure is formed by the connection of the target HIV DNA to loaded SNA.…”

Section: Biosensors For the Detection Of Pathogensmentioning

confidence: 99%

Recent advances in the biosensors application for the detection of bacteria and viruses in wastewater

Kadadou¹,

Tizani²,

Wadi³

et al. 2022

Journal of Environmental Chemical Engineering

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“…To achieve the trans-cleavage of the ssDNA reporter through the CRISPR/Cas system on electrochemical sensors, ssDNA was fixed onto the working gold electrode through Au-SH bonds [ [44] , [45] , [46] ]. Although stable, gold electrodes or screen-printed gold electrodes are more expensive than carbon-glass electrodes or screen-printed carbon electrodes, and the formation of Au-SH bond takes as long as 12 h [ [47] , [48] , [49] , [50] , [51] , [52] ]. To solve this problem, we employed CeO 2 nanorods and poly (allylamine hydrochloride) (PAH) to randomly immobilize the ssDNA onto a screen-printed carbon electrode (SPCE).…”

Section: Introductionmentioning

confidence: 99%