“…Few studies have detected, or focused on methods to detect, HIV in wastewater (Ansari et al, 1992; Kadadou et al, 2022; McCall et al, 2020; Preston et al, 1991). Wastewater surveillance of HIV in high-risk areas could facilitate an increase in status awareness, early application of retroactive and antiviral drugs, and decrease transmission of the viruses in communities.…”
Viruses of concern for quantitative wastewater monitoring are usually selected as a result of an outbreak and subsequent detection in wastewater. However, targeted metagenomics could proactively identify viruses of concern when used as an initial screening tool. To evaluate the utility of targeted metagenomics for wastewater screening, we used ViroCap, a panel of probes designed to target all known vertebrate viruses. Untreated wastewater was collected from wastewater treatment plants (WWTPs) and building-level manholes associated with vulnerable populations in Houston, TX. We evaluated differences in vertebrate virus detection between WWTP and building-level samples, classified human viruses in wastewater, and performed phylogenetic analysis on astrovirus sequencing reads to evaluate targeted metagenomics for subspecies level classification. Vertebrate viruses varied widely across building-level samples. Rarely detected and abundant viruses were identified in WWTP and building-level samples, including enteric, respiratory, and bloodborne viruses. Furthermore, full length genomes were assembled from astrovirus reads and two human astrovirus serotypes were classified in wastewater samples. This study demonstrates the utility of targeted metagenomics as an initial screening step for public health surveillance.
“…Few studies have detected, or focused on methods to detect, HIV in wastewater (Ansari et al, 1992; Kadadou et al, 2022; McCall et al, 2020; Preston et al, 1991). Wastewater surveillance of HIV in high-risk areas could facilitate an increase in status awareness, early application of retroactive and antiviral drugs, and decrease transmission of the viruses in communities.…”
Viruses of concern for quantitative wastewater monitoring are usually selected as a result of an outbreak and subsequent detection in wastewater. However, targeted metagenomics could proactively identify viruses of concern when used as an initial screening tool. To evaluate the utility of targeted metagenomics for wastewater screening, we used ViroCap, a panel of probes designed to target all known vertebrate viruses. Untreated wastewater was collected from wastewater treatment plants (WWTPs) and building-level manholes associated with vulnerable populations in Houston, TX. We evaluated differences in vertebrate virus detection between WWTP and building-level samples, classified human viruses in wastewater, and performed phylogenetic analysis on astrovirus sequencing reads to evaluate targeted metagenomics for subspecies level classification. Vertebrate viruses varied widely across building-level samples. Rarely detected and abundant viruses were identified in WWTP and building-level samples, including enteric, respiratory, and bloodborne viruses. Furthermore, full length genomes were assembled from astrovirus reads and two human astrovirus serotypes were classified in wastewater samples. This study demonstrates the utility of targeted metagenomics as an initial screening step for public health surveillance.
“…The main function of the biosensor is to select the substance to be measured. The main function of signal transducers is to convert the chemical effects generated by the interaction between the biological components and measured substances into electrical signals that can be considered as output [ 37 ]. Thus, it works in two steps.…”
Section: The Principle and Characteristics Of Biosensorsmentioning
In recent years, many different biosensors are being used to monitor physical health. Electrospun nanofiber materials have the advantages of high specific surface area, large porosity and simple operation. These properties play a vital role in biosensors. However, the mechanical properties of electrospun nanofibers are poor relative to other techniques of nanofiber production. At the same time, the organic solvents used in electrospinning are generally toxic and expensive. Meanwhile, the excellent performance of electrospun nanofibers brings about higher levels of sensitivity and detection range of biosensors. This paper summarizes the principle and application of electrospinning technology in biosensors and its comparison with other technologies.
“…Biosensors can be categorized as optical, mechanical, or electrochemical according to the transducers and response signals. Bioreceptors recognize viruses or variants of interest using specific probes such as enzymes, antibodies, and nucleic acids [ 5 ]. Thus far, bioreceptors have served as a key component in achieving high selectivity.…”
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