A signal processor made from DNA assembly and upconversion nanoparticle for pharmacokinetic study

Yao, Chunde; Tang, Jianpu; Zhu, Chenxu; Yang, Sen; Tang, Han; Dong, Luxi; Zhang, Chengqi; Tang, Qianyun; Liu, Peifeng; Yang, Dayong

doi:10.1016/j.nantod.2021.101352

Cited by 23 publications

(15 citation statements)

References 58 publications

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“…When studying the DNA supramolecular assembly on the interfaces of nanoscale particles, the chemical groups’ contribution in DNA molecules was considered to be more prominent. For example, the phosphate groups in DNA are able to form covalent bonds with ions, − and the hexameric rings of DNA bases can interact with other aromatic rings through π–π stacking . In addition, the DNA utilized for analysis can interact with DNA-formed nanoparticles through hydrogen bonds .…”

Section: Dna Assembly On the Interfaces Of Nanoscale Particlesmentioning

confidence: 99%

Section: Dna Assembly On the Interfaces Of Nanoscale Particlesmentioning

confidence: 99%

“…On the other hand, the supramolecular assembly of DNA on the interface of UCNPs is able to enrich DNA functional modules in a certain region, which is an effective means to improve the accuracy of bioanalysis. A DNA supramolecular assembly strategy at the nanoparticle interface was proposed, e.g., the assembly of ultralong DNA chains at the interface of UCNPs, to construct a nanoscale signal probe of the UCNP-DNA complex (UCDC) excited by near-infrared light . Through the exquisite design of the DNA sequence, the ultralong DNA chain was integrated with a hairpin region to load a chemotherapy drug, a DNA aptamer region to target tumor cells, and a conformational transformation region to release drug in response to a tumor microenvironment.…”

Section: Dna Assembly On the Interfaces Of Nanoscale Particlesmentioning

confidence: 99%

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DNA Supramolecular Assembly on Micro/Nanointerfaces for Bioanalysis

Yao

Tang

et al. 2022

Acc. Chem. Res.

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Metrics & MoreArticle Recommendations CONSPECTUS: Facing increasing demand for precision medicine, materials chemistry systems for bioanalysis with accurate molecular design, controllable structure, and adjustable biological activity are required. As a genetic biomacromolecule, deoxyribonucleic acid (DNA) is created via precise, efficient, and mild processes in life systems and can in turn precisely regulate life activities. From the perspective of materials chemistry, DNA possesses the characteristics of sequence programmability and can be endowed with customized functions by the rational design of sequences. In recent years, DNA has been considered to be a potential biomaterial for analysis and has been applied in the fields of bioseparation, biosensing, and detection imaging. To further improve the precision of bioanalysis, the supramolecular assembly of DNA on micro/nanointerfaces is an effective strategy to concentrate functional DNA modules, and thus the functions of DNA molecules for bioanalysis can be enriched and enhanced. Moreover, the new modes of DNA supramolecular assembly on micro/nanointerfaces enable the integration of DNA with the introduced components, breaking the restriction of limited functions of DNA materials and achieving more precise regulation and manipulation in bioanalysis. In this Account, we summarize our recent work on DNA supramolecular assembly on micro/nanointerfaces for bioanalysis from two main aspects. In the first part, we describe DNA supramolecular assembly on the interfaces of microscale living cells. The synthesis strategy of DNA is based on rolling-circle amplification (RCA), which generates ultralong DNA strands according to circular DNA templates. The templates can be designed with complementary sequences of functional modules such as aptamers, which allow DNA to specifically bind with cellular interfaces and achieve efficient cell separation. In the second part, we describe DNA supramolecular assembly on the interfaces of nanoscale particles. DNA sequences are designed with functional modules such as targeting, drug loading, and gene expression and then are assembled on interfaces of particles including upconversion nanoparticles (UCNPs), gold nanoparticles (AuNPs), and magnetic nanoparticle (MNPs). The integration of DNA with these functional particles achieves cell manipulation, targeted tumor imaging, and cellular regulation. The processes of interfacial assembly are well controlled, and the functions of the obtained bioanalytical materials can be flexibly regulated. We envision that the work on DNA supramolecular assembly on micro/nanointerfaces will be a typical paradigm for the construction of more bioanalytical materials, which we hope will facilitate the development of precision medicine.

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Section: Dna Assembly On the Interfaces Of Nanoscale Particlesmentioning

confidence: 99%

Section: Dna Assembly On the Interfaces Of Nanoscale Particlesmentioning

confidence: 99%

Section: Dna Assembly On the Interfaces Of Nanoscale Particlesmentioning

confidence: 99%

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DNA Supramolecular Assembly on Micro/Nanointerfaces for Bioanalysis

Yao

Tang

et al. 2022

Acc. Chem. Res.

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“…Under the action of DNA ligase (such as T4 DNA ligase or Taq DNA ligase) and a single strand primer that is partially complementary to the 5′ and 3′ ends of the template, the end of 5′ phosphoric acid (5′-P) and end of 3′ hydroxyl (3′-OH) of the DNA template are linked and cycled to form a circular template for subsequent amplification. 67 Circular template amplification relies on DNA polymerase with chain displacement and continuous synthesis activity. Phi29 DNA polymerase and Bst DNA polymerase are the most commonly used.…”

Section: Mechanism and Classification Of Rca Technologymentioning

confidence: 99%

Recent advances in biological detection with rolling circle amplification: design strategy, biosensing mechanism, and practical applications

Gao

Huang²,

Wang

et al. 2022

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“…Tang et al constructed a DNA/UCNP nanocomplex to achieve NIR-excited real-time drug-release monitoring. 62 Firstly, the circular DNA template was interfacially assembled onto the surface of UCNP. Then the ultra-long single-stranded DNA was synthesized by RCA which contained multiple repeated functional modules including aptamer sequence, drug loading sequence, and response sequence.…”

Section: Introductionmentioning

confidence: 99%