Construction of rolling circle amplification-based DNA nanostructures for biomedical applications

Abstract

DNA-based materials exhibit great potential in biomedical applications due to the excellent sequence programmability and unique functional designability. Rolling circle amplification (RCA) is an efficient isothermal enzymatic amplification strategy to...

Bottlebrush DNA-Primed Rolling Circle Amplification for Sensitive Detection of Biomolecules

“…Clearly, ultrasensitive detection of biomolecules, especially those of low abundance, can be achieved with this BDP-RCA-based detection method, and the detection sensitivity can still be further increased by associating the BDP-RCA with other advanced means for signal generation, such as electrochemiluminescence and electrochemical methods, instead of fluorescence of the stained BDP-RCA product. , The RCA technique has been widely used for signal amplification in biosensing, − and the BDP-RCA method developed in this work produces the amplification products much more rapidly than the regular RCA, which significantly increases the gain of the signal in a limited time, and greatly shortens the time needed for detecting a certain target. Although a similar approach, branched RCA technique has also been developed recently and used in several detection applications, , in branched RCA, the side DNA chains in the product are attached to the main DNA chain via hybridization of the complementary sequences.…”

“…Once BDs have been produced, to expand their properties and functions, the single-stranded DNA (ssDNA) side chains may be further elongated enzymatically, for example, via rolling circle amplification (RCA). The RCA technique uses a circular ssDNA as the template to synthesize DNA strands containing repeated sequences that are reverse complementary to the template and has been broadly applied in signal amplification for biosensing and production of DNA nanostructures and biomaterials. − Combined use of BDs and the RCA technique may lead to the construction of novel nanomaterials and the development of novel biosensing tools.…”

Amplification of an Electrochemiluminescence-Emissive Aptamer into DNA Nanotags for Sensitive Fecal Calprotectin Determination

“…26,27 Here, by virtue of the editable multitasking imprints in DNA machineries alongside their highly evolved accuracy for additive manufacturing, 26 a completely DNA-scaffold ECL luminophore was replicated out of the living course of rolling circle amplification (RCA). 28 Its primer/amplicons carried the aptameric sequence of a zinc protoporphyrin IX (ZnPPIX) photoreactive center, 29 which tailgated at periodical intervals while in step with the persistent extension until a final closepacked coil of DNA nanoflowers (dubbed ZnPDFs; Scheme 1A). Rightly like their original utilities in drug delivery, 30 such a clockwork biocatalysis did not just rallied singlet guests in spontaneity to a monolithic yet safe-distancing colony (least chance of π-stacking so as to minimize the excitonic energy losses) but also spliced monoclonal epitopes in good coherence that deployed seamlessly throughout the peripherals.…”

“…The as-demonstrated ECL activity of ZnPDFs awaits multifactorial interrogations for a ready and robust response toward biosensing applications (Prosthetic Group Screening and Condition Optimization in the Supporting Information and Figure S1 and S2). The open programming frame in RCA endows ZnPDFs with writable recognition modules, 28 such as the insertion of other functional strands, which innately avails the access to a kaleidoscope of biointerplays. Given wellcharacterized ECL multiplications from ZnPDF I , its mutant ZnPDF III was tailored further, which overhangs the binder of calprotectin.…”

“…Here, by virtue of the editable multitasking imprints in DNA machineries alongside their highly evolved accuracy for additive manufacturing, a completely DNA-scaffold ECL luminophore was replicated out of the living course of rolling circle amplification (RCA) . Its primer/amplicons carried the aptameric sequence of a zinc protoporphyrin IX (ZnPPIX) photoreactive center, which tailgated at periodical intervals while in step with the persistent extension until a final close-packed coil of DNA nanoflowers (dubbed ZnPDFs; Scheme A).…”