A signal-on homogeneous electrochemical biosensor for sequence-specific microRNA based on duplex-specific nuclease-assisted target recycling amplification

Chen, Fu; Liu, Chang; Wang, Shaoyun; Luo, Fang; Lin, Zhenyu

doi:10.1039/c6ay02039a

Cited by 10 publications

(4 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The detection with increasing response signals based on “OFF-ON Signal” was also demonstrated in the case of the release of the labeled DNA probe. Thus, Fu et al [ 56 ] developed a biosensor based on a homogenous system of detection without immobilization of the ssDNA probe, permitting the reduction in the preparation time of the biosensor. Indeed, the biosensor is based on the negatively charged probes marked with MB at the 3’ end and a negatively charged indium tinoxide (ITO) electrode surface where DNA cannot diffuse easily to the surface due to electrostatic repulsion and the low electrochemical signal of MB is detected.…”

Section: Electrochemical Biosensor Based On Electroactive Labeled mentioning

confidence: 99%

Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons

et al. 2020

View full text Add to dashboard Cite

Cancer is the second most fatal disease in the world and an early diagnosis is important for a successful treatment. Thus, it is necessary to develop fast, sensitive, simple, and inexpensive analytical tools for cancer biomarker detection. MicroRNA (miRNA) is an RNA cancer biomarker where the expression level in body fluid is strongly correlated to cancer. Various biosensors involving the detection of miRNA for cancer diagnosis were developed. The present review offers a comprehensive overview of the recent developments in electrochemical biosensor for miRNA cancer marker detection from 2015 to 2020. The review focuses on the approaches to direct miRNA detection based on the electrochemical signal. It includes a RedOx-labeled probe with different designs, RedOx DNA-intercalating agents, various kinds of RedOx catalysts used to produce a signal response, and finally a free RedOx indicator. Furthermore, the advantages and drawbacks of these approaches are highlighted.

show abstract

Section: Electrochemical Biosensor Based On Electroactive Labeled mentioning

confidence: 99%

Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons

et al. 2020

View full text Add to dashboard Cite

show abstract

“…For example, Hou and colleagues designed a signal-off homogeneous electrochemical sensor for the detection of miRNA using a hybridization chain reaction [25]. Our group earlier reported a homogeneous electrochemical biosensor using a duplex-specific nuclease (DSN) cycle target to achieve signal amplification for miRNA detection [26]. But the sensitivity of these biosensors is not high enough for real sample detection since the signal amplification technique had been used.…”

Section: And His Coworkers Prepared the Ultrathinmentioning

confidence: 99%

Homogeneous electrochemical biosensor for microRNA based on enzyme-driven cascaded signal amplification strategy

Huang

Zheng

et al. 2020

Anal Bioanal Chem

Self Cite

View full text Add to dashboard Cite

Infectious diseases are a long-standing and severe global public health problem. The rapid diagnosis of infectious diseases is an urgent need to solve this problem. MicroRNA (miRNA) plays an important role in the intervention of some infectious diseases and is expected to become a potential biomarker for the diagnosis and prognosis of infectious diseases. It is of great significance to develop rapid and sensitive methods for detecting miRNA for effective control of infectious diseases. In this study, a simple and highly sensitive homogeneous electrochemical method for microRNAs using enzyme-driven cascaded signal amplification has been developed. In the presence of target miRNA, the reaction system produced plenty of MB-labeled single-nucleotide fragments (MB-MF) containing a few negative charges, which can diffuse to the negative surface of the ITO electrode easily, so an obvious electrochemical signal enhancement was obtained. Without the target, MB-HP contains a relatively large amount of negative charges due to the phosphates on the DNA chain, which cannot be digested by the enzyme and cannot diffuse freely to the negatively charged ITO electrode, so only a small signal was detected. The enhanced electrochemical response has a linear relationship with the logarithm of miRNA concentration in the range of 10 fM to 10 nM and the limit of detection as low as 3.0 fM. Furthermore, the proposed strategy showed the capability of discriminating single-base mismatch and performed eligibly in the analysis of miRNA in cell lysates, exhibiting great potential for disease diagnosis and biomedical research.

show abstract

“…The DNA methylation level was monitored via recording the voltammetric signal change of methylene blue at a AuNP-modified electrode, and simultaneously this sensor also could be used for the analysis of methyltransferase activity. 444 To improve the sensitivity of nowash electrochemical biosensing methods, some effective signal amplification strategies, such as enzyme-based signal amplifica- tion (e.g., exonuclease I or III, 434,445 nicking endonuclease, 446 DSN, 447 DNA polymerase, 448 and enzyme-free signal amplification (HCR) 449 ), were introduced, and these amplified nowash DPV-based electrochemical biosensors were successfully used for ultrasensitive detection of cancer-related DNA, miRNA, and protein. For example, an affinity-based electrochemical sensor created by GO-modified glassy carbon electrode was developed for no-wash detection of CEA by Ge et al, 450 who used the aptamer as a recognition element and methylene blue as an electrochemical probe for DPV coupled with T7 exonuclease-assisted target recycling amplification (Figure 16A).…”

Section: Electrochemical No-wash Biosensorsmentioning

confidence: 99%

Nanotechnology-Enhanced No-Wash Biosensors for in Vitro Diagnostics of Cancer

et al. 2017

View full text Add to dashboard Cite

In vitro biosensors have been an integral component for early diagnosis of cancer in the clinic. Among them, no-wash biosensors, which only depend on the simple mixing of the signal generating probes and the sample solution without additional washing and separation steps, have been found to be particularly attractive. The outstanding advantages of facile, convenient, and rapid response of no-wash biosensors are especially suitable for point-of-care testing (POCT). One fast-growing field of no-wash biosensor design involves the usage of nanomaterials as signal amplification carriers or direct signal generating elements. The analytical capacity of no-wash biosensors with respect to sensitivity or limit of detection, specificity, stability, and multiplexing detection capacity is largely improved because of their large surface area, excellent optical, electrical, catalytic, and magnetic properties. This review provides a comprehensive overview of various nanomaterial-enhanced no-wash biosensing technologies and focuses on the analysis of the underlying mechanism of these technologies applied for the early detection of cancer biomarkers ranging from small molecules to proteins, and even whole cancerous cells. Representative examples are selected to demonstrate the proof-of-concept with promising applications for in vitro diagnostics of cancer. Finally, a brief discussion of common unresolved issues and a perspective outlook on the field are provided.

show abstract

A signal-on homogeneous electrochemical biosensor for sequence-specific microRNA based on duplex-specific nuclease-assisted target recycling amplification

Abstract: A signal-on homogeneous electrochemical biosensor for sequence-specific microRNA (miRNA) based on duplex-specific nuclease (DSN) assisted target recycling amplification has been developed.

Cited by 10 publications

References 46 publications

Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons

Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons

Homogeneous electrochemical biosensor for microRNA based on enzyme-driven cascaded signal amplification strategy

Nanotechnology-Enhanced No-Wash Biosensors for in Vitro Diagnostics of Cancer

Contact Info

Product

Resources

About