A sialochemical study on patients with Sjögren's syndrome

Benedek-Spät, Erika; Berényi, B; Csiba, A

doi:10.1016/0003-9969(75)90132-6

Cited by 19 publications

(4 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In general, concentrations of cathode-migrating proteins increased in SS, but another feature was one or two additional bands in the anodal region. In unstimulated parotid saliva from two patients with SS, similar additional bands were found (64).…”

Section: Proteinssupporting

confidence: 53%

“…The abnormal proteins were not identified; despite this it was concluded that "such information would be invaluable and lead to a clear understanding of the nature of salivary gland abnormalities in connective tissue disease at the molecular level" (62). In one group of patients including both primary and secondary SS characteristic quantitative and qualitative changes in protein composition of stimulated parotid saliva were found by BENEDAK-SPAT et al (64). The findings were compared with those of healthy controls and of patients with sialosis.…”

Section: Proteinsmentioning

confidence: 95%

See 1 more Smart Citation

Sialochemistry in Sjögren's syndrome: a review

Thorn

Prause

Oxholm

1989

J Oral Pathology Medicine

View full text Add to dashboard Cite

Sjögren's syndrome (SS) is an autoimmune exocrinopathy. The salivary glands are the site of activated T- and B-lymphocytes, along with gradual parenchymal destruction, diminished flow and altered composition of the secretory product. At present, sialochemistry has achieved no significance for the evaluation of SS patient. However, the number of sialochemical publications is steadily growing. This study review current sialochemical findings in patients with SS and relate the observations to the present concept of diagnosis, pathogenesis and prognosis of SS. An ideal combination of the collection of low-stimulated pure secretion, measurements of absolute flow-rates, and biopsy from the same glands seem to be unobtainable in SS patients. But two procedures may be appropriate: stimulated parotid secretion combined with parotid biopsies, or absorbance of low-stimulated labila saliva combined with labial gland biopsy. Sufficient data on disease-specific alterations in salivary composition in SS are still lacking. However, detection of specific changes in protein synthesis or in glycosylation as well as the detection of inflammatory cell products should be possible with the use of sensitive biochemical assays.

show abstract

Section: Proteinssupporting

confidence: 53%

Section: Proteinsmentioning

confidence: 95%

Sialochemistry in Sjögren's syndrome: a review

Thorn

Prause

Oxholm

1989

J Oral Pathology Medicine

View full text Add to dashboard Cite

show abstract

“…Many changes in SS salivary constituents have been described previously, suggesting that saliva could be used to diagnose the syndrome. Increased concentrations of Na þ , Cl - [5,15,16], IgG [16], lysozyme [17], matrix metalloproteinase (MMP)-2 and MMP-9 [18] in parotid saliva; lactoferrin [19][20][21], IgA [16,22,23], 2-microglobulin [24][25][26][27], albumin [16,23] in both parotid and whole saliva; and kallikrein [28] and cystatins C and S [23] in whole saliva have been reported. The present study compared the salivary proteomes of SS and non-SS patient groups using modern proteomic methods (SELDI-TOF-MS and 2D-DIGE) to identify the most significantly different salivary biomarkers for future studies testing their diagnostic potential.…”

mentioning

confidence: 99%

Identification of parotid salivary biomarkers in Sjögren's syndrome by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry and two-dimensional difference gel electrophoresis

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Assessment of salivary gland function is, therefore, of potential diagnostic importance. [1][2][3][4][5][6][7][8][9][10][11][12] Methods for determining salivary gland (dys)function include salivary flow rate measurements (sialometry) and analysis of salivary composition (sialochemistry), for which whole saliva (oral fluid) is most frequently used. The accuracy of these techniques, however, can be improved considerably by using glandular saliva rather than whole saliva.…”

mentioning

confidence: 99%

Sialometry and sialochemistry: a non-invasive approach for diagnosing Sjogren's syndrome

Kalk

Vissink²,

Stegenga³

et al. 2002

Annals of the Rheumatic Diseases

View full text Add to dashboard Cite

Background: Analysis of salivary variables has frequently been proposed as a diagnostic tool for Sjö-gren's syndrome (SS). Because univocal salivary reference values are lacking, it is currently rather difficult to use sialometry and sialochemistry for diagnosing SS unless major changes have occurred in salivary secretion and composition. Objective: To define reference values of several salivary variables, which offer a possible new and non-invasive means of diagnosing SS. Methods: Cut off points were selected from receiver operating characteristic curves of gland-specific sialometrical and sialochemical variables, which have proved to be potentially relevant for diagnosing SS in a previous study-that is, sodium, chloride, and phosphate concentration in stimulated parotid and submandibular/sublingual (SM/SL) saliva, unstimulated and stimulated SM/SL flow rates, and lag phase of parotid secretion, respectively. By combining the most discriminating variables, two different diagnostic approaches for SS were applied in a group of 100 patients and subsequently evaluated in a second group of 20 patients. The first approach was to combine variables by applying their cut off points into sets of criteria for a positive diagnosis of SS. The second approach was to construct a logistic regression model that predicts the true state of a patient (SS or non-SS). From both approaches, the tests with highest likelihood ratio combined with the smallest number of rejected cases were selected for clinical use. Results: The most accurate test combined the stimulated SM/SL flow rate and parotid sodium and chloride concentration as salivary variables for diagnosing SS; it had a sensitivity of 0.85 and a specificity of 0.96. The selected tests proved equally accurate in the second group of patients. Conclusions: Because the proposed non-invasive diagnostic tools can be easily applied, do not need a laboratory other than for routine blood testing, and are very accurate, gland-specific sialometry and sialochemistry may eventually replace other, more invasive, diagnostic techniques for diagnosing SS.

show abstract

A sialochemical study on patients with Sjögren's syndrome

Cited by 19 publications

References 13 publications

Sialochemistry in Sjögren's syndrome: a review

Sialochemistry in Sjögren's syndrome: a review

Identification of parotid salivary biomarkers in Sjögren's syndrome by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry and two-dimensional difference gel electrophoresis

Sialometry and sialochemistry: a non-invasive approach for diagnosing Sjogren's syndrome

Contact Info

Product

Resources

About