Sialochemistry in Sjögren's syndrome: a review

Thorn, Jens Jørgen; Prause, Jan Ulrik; Oxholm, P

doi:10.1111/j.1600-0714.1989.tb01343.x

Cited by 33 publications

(22 citation statements)

References 115 publications

(88 reference statements)

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“…The presence of these two phenomena leads to a diagnosis of primary SS. In secondary SS, a well-defined connective tissue disease (most commonly rheumatoid arthritis or systemic lupus erythematosus) is present in addition to the xerostomia and/or the keratoconjunctivitis (Schiødt and Thorn, 1989;Thorn et al ., 1989). In addition to involvement of the salivary and lacrimal glands, SS may also affect the skin, lungs, liver, kidneys, thyroid, and nervous system (Talal, 1992).…”

Section: Autoimmunediseases -S Jögren ' S Syndromementioning

confidence: 99%

The Diagnostic Applications of Saliva— A Review

Kaufman

Lamster

2002

Critical Reviews in Oral Biology & Medicine

785

618

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This review examines the diagnostic application of saliva for systemic diseases. As a diagnostic fluid, saliva offers distinctive advantages over serum because it can be collected non-invasively by individuals with modest training. Furthermore, saliva may provide a cost-effective approach for the screening of large populations. Gland-specific saliva can be used for diagnosis of pathology specific to one of the major salivary glands. Whole saliva, however, is most frequently used for diagnosis of systemic diseases, since it is readily collected and contains serum constituents. These constituents are derived from the local vasculature of the salivary glands and also reach the oral cavity via the flow of gingival fluid. Analysis of saliva may be useful for the diagnosis of hereditary disorders, autoimmune diseases, malignant and infectious diseases, and endocrine disorders, as well as in the assessment of therapeutic levels of drugs and the monitoring of illicit drug use.

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Section: Autoimmunediseases -S Jögren ' S Syndromementioning

confidence: 99%

The Diagnostic Applications of Saliva— A Review

Kaufman

Lamster

2002

Critical Reviews in Oral Biology & Medicine

785

618

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“…Neither the concentration nor the output of total protein in stimulated parotid saliva were significantly different in the patients with secondary Sjögren©s syndrome compared to the normal controls. A review of eight studies of parotid salivary protein secretion by Thorn et al [23] revealed that compared to healthy controls, Sjögren©s syndrome protein concentration can be increased (two studies), decreased (one study) or remain unchanged (five studies). In our examination of protein composition in the different subject groups there was great variation in protein banding patterns between subjects, as revealed by FITC staining of nitrocellulose blots.…”

Section: Discussionmentioning

confidence: 97%

Lectin binding studies of parotid salivary glycoproteins in Sjögren's syndrome

Carpenter¹,

Pankhurst²,

Proctor³

1999

Electrophoresis

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Human parotid salivas were collected from patients with secondary Sjögren's syndrome and controls without disease or with drug‐induced xerostomia. Parotid glycoproteins were separated by gradient sodium dodecyl sulphate gel electrophoresis (SDS‐PAGE), electroblotted onto nitrocellulose membrane and probed with biotinylated lectins of characterised sugar specificities. The binding patterns of lectins from Maclura pomifera (MPA) and Arachis hypogaea (PNA) indicated that many parotid glycoproteins have sialylated O‐linked glycans and that sialylation is not affected by disease. Binding by lectins from Ricinus communis (RCA‐1), Limax flavus (LFA), Lotus tetragonolobus (LTA) and Ulex europaeus (UEA‐1) appeared unaltered in secondary Sjögren's syndrome, suggesting no obvious change in N‐glycosylation of parotid glycoproteins. Variations in binding patterns of most lectins was attributable to subject‐to‐subject variations in recognised polymorphic proteins. Dolichos biflorus agglutinin (DBA) consistently showed increased binding to a 75 kDa (Mr) protein in salivas from patients with secondary Sjögren's syndrome. The binding protein was identified as lactoferrin but found not to contain N‐acetylgalactosamine, the sugar to which DBA binds. Binding of DBA to lactoferrin was dependent upon its saturation with iron, modified SDS‐PAGE under nonreducing conditions resolved iron‐free and iron‐saturated lactoferrins and demonstrated increased levels of the iron‐saturated form in secondary Sjögren's syndrome. Lectin binding studies of purified lactoferrins from saliva, milk, and polymorphonuclear neutrophils suggested that raised levels of lactoferrin in saliva originate from salivary cells and not from inflammatory cells. These results suggest that DBA binding provides greater specificity as an indicator of salivary gland disease than measurement of lactoferrin levels alone.

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“…Determination of [32-m, LZM and Lf in saliva has frequently been proposed for diagnostic use in primary SS but so far insufficient data have prevented its clinical use (11)(12)(13)(14)(19)(20)(21). To our knowledge this is the first study to establish simultaneously the diagnostic value of salivary fluid levels of [32-m, LZM and Lf for primary SS.…”

Section: Discussionmentioning

confidence: 99%

“…of 132-microglobulin (132-m), lysozyme (LZM), and lactoferrin (Lf) has been proposed for this goal (11)(12)(13)(14).…”

Section: Introductionmentioning

confidence: 99%

The diagnostic value of salivary fluid levels of β2-microglubulin, lysozyme and lactoferrin for primary Sjögren's synrome

Markusse¹,

Otten

Vroom³

et al. 1992

Clin Rheumatol

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In search of a simple non-invasive diagnostic test for primary Sjögren's syndrome (SS) the concentration of beta 2-microglobulin (beta 2-m), lysozyme (LZM) and lactoferrin (Lf) was measured in stimulated parotid saliva of 39 patients with primary SS, 42 patients suspected of the syndrome in whom the diagnosis could be excluded (NON-SS) and in 41 normal control individuals. Salivary fluid levels of beta 2-m, LZM and Lf exceeding the mean + 2 x standard deviation of healthy control values were found in respectively 58%, 23%, and 26% of the primary SS patients and in 7%, 11% and 0% of the NON-SS patients. The results of this study indicate that due to the low sensitivity the tests are not suitable as a screening procedure for patients suspected of having primary SS. However, measurement of beta 2-m in stimulated parotid saliva may be used as an adjunctive diagnostic test for primary SS.

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Sialochemistry in Sjögren's syndrome: a review

Cited by 33 publications

References 115 publications

The Diagnostic Applications of Saliva— A Review

The Diagnostic Applications of Saliva— A Review

Lectin binding studies of parotid salivary glycoproteins in Sjögren's syndrome

The diagnostic value of salivary fluid levels of β2-microglubulin, lysozyme and lactoferrin for primary Sjögren's synrome

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